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Enzynomics co Ltd top scripttm rt reverse transcription kit
Top Scripttm Rt Reverse Transcription Kit, supplied by Enzynomics co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+scripttm+reverse+transcriptase+kit/topscript+reverse+transcription+kit/pm39979315-101-10-16
Average 90 stars, based on 1 article reviews
top scripttm rt reverse transcription kit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Bone morphogenic protein 9 is a novel thermogenic hepatokine secreted in response to cold exposure.
Article Snippet: Primary mouse hepatocytes were obtained from 8- to 10-week-old male C57BL/6J mice and prepared via a two-step perfusion process using liver perfusion media and liver digestion buffer (Life Technologies) as described previously [23].

Article Title: Activin E is a new guardian protecting against hepatic steatosis via inhibiting lipolysis in white adipose tissue
Article Snippet: The RNA was subsequently reverse-transcribed to cDNA by using a TOPscriptTM reverse transcription (RT) kit (Enzynomics, Korea).

Article Title: Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate.
Article Snippet: Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules.. These characteristics of GO can promote the interactions between the substrates and cell surfaces.. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs).

Incubation:

Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV
Article Snippet: In vitro transcription was performed using the EZTM T7 high-yield in vitro transcription kit (Enzynomics, Daejeon, South Korea). .. For transcription using the Enzynomics Transcription kit, a 3 μg linearized DNA template was incubated with T7 transcription buffer, MgCl 2 , 10 mM DTT, enhancer solution, 5 mM rNTP, nuclease-free water, and 200 U T7 enzyme mix for 1 h at 37 °C. .. The transcripts were incubated with RNase-free DNase I (Enzynomics) for 30 min at 37 °C, followed by termination of the reaction by incubation at 65 °C for 10 min. RNA purification was performed using the LiCl method.

Article Title: Heterologous vaccination utilizing viral vector and protein platforms confers complete protection against SFTSV.
Article Snippet: In vitro transcription was performed using the EZTM T7 high-yield in vitro transcription kit (Enzynomics, Daejeon, South Korea). .. For transcription using the Enzynomics Transcription kit, a 3 μg linearized DNA template was incubated with T7 transcription buffer, MgCl2, 10 mM DTT, enhancer solution, 5 mM rNTP, nuclease-free water, and 200 U T7 enzyme mix for 1 h at 37 °C. .. The transcripts were incubated with RNase-free DNase I (Enzynomics) for 30 min at 37 °C, followed by termination of the reaction by incubation at 65 °C for 10 min. RNA purification was performed using the LiCl method.

Reverse Transcription:

Article Title: Predictive roles of Asprosin and Gremlin-1 expression in Egyptian pediatric patients with type 1 diabetes.
Article Snippet: One ng of RNA was used for each reverse transcription procedure, and the quantity and quality of the RNA were both measured using the Nanodrop 2000 spectrophotometer (Thermo Fisher, Waltham, MA, USA). .. Reverse transcription The cDNA was produced from it using the Top ScriptTM RT reverse transcription kit (Enzynomics Inc., Korea) (catalogue number: RT 220), as directed by the manufacturer. .. Real-time PCR The Asprosin and Gremlin-1 gene expression were determined using quantitative realtime PCR (RT-qPCR) using a StepOneTM System real-time PCR apparatus (Applied Biosystems, Foster City, CA, USA) and the TOPrealTM qPCR 2X Pre-MIX (SYBR Green with low ROX) kit (Enzynomics Inc., Korea) (catalogue number: RT500s).

Article Title: N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis
Article Snippet: Total RNA was isolated from cumulus cells or ZP-free oocytes and embryos using the Trizol reagent (Invitrogen), according to the manufacturer’s protocol. .. The cDNA was synthesized using the TOPscript Reverse Transcription kit (Enzynomics Co. Ltd., Daejeon, Korea). ..

Article Title: Predictive roles of Asprosin and Gremlin-1 expression in Egyptian pediatric patients with type 1 diabetes
Article Snippet: One ng of RNA was used for each reverse transcription procedure, and the quantity and quality of the RNA were both measured using the Nanodrop 2000 spectrophotometer ( Thermo Fisher , Waltham , MA , USA ). .. Reverse transcription The cDNA was produced from it using the Top Script TM RT reverse transcription kit ( Enzynomics Inc. , Korea ) (catalogue number: RT 220), as directed by the manufacturer. .. Real-time PCR The Asprosin and Gremlin-1 gene expression were determined using quantitative real-time PCR (RT-qPCR) using a StepOne TM System real-time PCR apparatus ( Applied Biosystems , Foster City , CA , USA ) and the TOPreal TM qPCR 2X Pre-MIX (SYBR Green with low ROX) kit ( Enzynomics Inc. , Korea ) (catalogue number: RT500s).

Produced:

Article Title: Predictive roles of Asprosin and Gremlin-1 expression in Egyptian pediatric patients with type 1 diabetes.
Article Snippet: One ng of RNA was used for each reverse transcription procedure, and the quantity and quality of the RNA were both measured using the Nanodrop 2000 spectrophotometer (Thermo Fisher, Waltham, MA, USA). .. Reverse transcription The cDNA was produced from it using the Top ScriptTM RT reverse transcription kit (Enzynomics Inc., Korea) (catalogue number: RT 220), as directed by the manufacturer. .. Real-time PCR The Asprosin and Gremlin-1 gene expression were determined using quantitative realtime PCR (RT-qPCR) using a StepOneTM System real-time PCR apparatus (Applied Biosystems, Foster City, CA, USA) and the TOPrealTM qPCR 2X Pre-MIX (SYBR Green with low ROX) kit (Enzynomics Inc., Korea) (catalogue number: RT500s).

Article Title: Predictive roles of Asprosin and Gremlin-1 expression in Egyptian pediatric patients with type 1 diabetes
Article Snippet: One ng of RNA was used for each reverse transcription procedure, and the quantity and quality of the RNA were both measured using the Nanodrop 2000 spectrophotometer ( Thermo Fisher , Waltham , MA , USA ). .. Reverse transcription The cDNA was produced from it using the Top Script TM RT reverse transcription kit ( Enzynomics Inc. , Korea ) (catalogue number: RT 220), as directed by the manufacturer. .. Real-time PCR The Asprosin and Gremlin-1 gene expression were determined using quantitative real-time PCR (RT-qPCR) using a StepOne TM System real-time PCR apparatus ( Applied Biosystems , Foster City , CA , USA ) and the TOPreal TM qPCR 2X Pre-MIX (SYBR Green with low ROX) kit ( Enzynomics Inc. , Korea ) (catalogue number: RT500s).

Synthesized:

Article Title: N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis
Article Snippet: Total RNA was isolated from cumulus cells or ZP-free oocytes and embryos using the Trizol reagent (Invitrogen), according to the manufacturer’s protocol. .. The cDNA was synthesized using the TOPscript Reverse Transcription kit (Enzynomics Co. Ltd., Daejeon, Korea). ..



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