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Biouniquer Technology Co Ltd bu-script rt kit
Bu Script Rt Kit, supplied by Biouniquer Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+script+rt+kit/bu+script+rt+kit/pmc03989268-111-18-21
Average 90 stars, based on 1 article reviews
bu-script rt kit - by Bioz Stars, 2026-11
90/100 stars

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Expressing:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

Isolation:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

Reverse Transcription:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

Real-time Polymerase Chain Reaction:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

SYBR Green Assay:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

Microarray:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).

Synthesized:

Article Title: Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Article Snippet: The reverse transcription was performed with a BU-Script KIT(50) (Biouniquer Technology Company, Nanjing, China) as follows (in 20 μL): 4 μL 5× BU-Script buffer, 1 μL dNTP mix (10 mmol/L), 1 μL Oligo (dT) 18 (10 pmol/μL), 1 μL 20 U/μL RNase Inhibitor, 1 μL 20 U/μL BU-Script Reverse Transcriptase and 2 μL total RNA(1 μg/μL) and DEPC water were added to adjust the final volume to 20 μL.

Article Title: Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin
Article Snippet: To detect miR1276 expression, the total RNA from the cells was isolated with Trizol (Invitrogen) and reverse transcribed using a BU-Script RT Kit (Biouniquer, Nanjing) in a 20-μL system.

Article Title: Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.
Article Snippet: While adriamycin (adr) offers improvement in survival for breast cancer (BCa) patients, unfortunately, drug resistance is almost inevitable.. Mounting evidence suggests that exosomes act as a vehicle for genetic cargo and constantly shuttle biologically active molecules including microRNAs (miRNAs) between heterogeneous populations of tumor cells, engendering a resistance-promoting niche for cancer progression.. Our recent study showed that exosomes from docetaxelresistance BCa cells could modulate chemosensitivity by delivering miRNAs.

Article Title: d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
Article Snippet: Briefly, cDNA for miRNA was synthesized using the BU-Script RT Kit (Biouniquer Technology, Nanjing, China) on an iCycler iQ system (Bio-Rad, U.S.A.), and specific stem-loop primers (Springen Biotechnology, Nanjing, China) were designed for the selected miRNAs.

Article Title: MicroRNA-3646 Contributes to Docetaxel Resistance in Human Breast Cancer Cells by GSK-3β/β-Catenin Signaling Pathway
Article Snippet: RNA was reversely transcribed using the BU-Script RT Kit (Biouniquer Technology Co, Ltd, Nanjing, China) according to manufacturer's recommendations.

Article Title: Exosomes from docetaxel-resistant breast cancer cells alter chemosensitivity by delivering microRNAs.
Article Snippet: Breast cancer (BCa) remains chemo-unresponsive by inevitable progression of resistance to first-line treatment with docetaxel (doc).. Emerging studies indicate that exosomes act as mediators of intercellular communication between heterogeneous populations of tumor cells, engendering a transmitted drug resistance for cancer development.. Such modulatory effects have been related to the constant shuttle of biologically active molecules including microRNAs (miRNAs).

Article Title: Sulforaphane activates the cerebral vascular Nrf2-ARE pathway and suppresses inflammation to attenuate cerebral vasospasm in rat with subarachnoid hemorrhage.
Article Snippet: Nrf2–ARE pathway reportedly plays a protective role in several central nervous system diseases.. No study has explored the role of the Nrf2–ARE pathway in cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).. The purpose of the present study was to investigate the activation of the cerebral vascular Nrf2–ARE pathway and to determine the potential role of this pathway in the development of CVS following SAH.

Article Title: D Rhamnose β-Hederin against human breast cancer by reducing tumor-derived exosomes
Article Snippet: Then, bound proteins were visualized using the ECL Plus kit (EMD Millipore, Billerica, MA, USA) with Image Lab Software version 5.2.1 (Bio-Rad, USA).



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