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Promega go script kit
Go Script Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+script+reverse+kit/go+script+kit/pmc10940650-65-0-3
Average 90 stars, based on 1 article reviews
go script kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: NUPR1 inhibitor ZZW-115 induces ferroptosis in a mitochondria-dependent manner
Article Snippet: AUC values were calculated by nonlinear regression curves with a robust fit using GraphPad software. .. Total RNA was extracted from cells using Trizol kit (Invitrogen, Carlsbad, CA, USA) and cDNA was obtained by reverse-transcribed using Go Script kit (Promega), according to the manufacturer’s instructions. .. Real-time quantitative PCR (qRT-PCR) was performed using Stratagene MXPro-MX3005P system.

Article Title: Feeding conditions and breed affect the level of DNA methylation of the mitochondrial uncoupling protein 3 gene in chicken breast muscle.
Article Snippet: To investigate the effects of feed condition and breed on the level of DNA methylation for the uncoupling protein 3 (UCP3) gene, which is an important candidate gene for regulating intramuscular fat (IMF) content in chicken breast muscle, breast muscle of Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feed and captivity-feed conditions was analyzed.. Using RNA sequencing, 47 and 113 candidate genes were determined to be related to feed conditions and breed, respectively, and 7 differentially expressed genes were confirmed by real-time quantitative PCR, including UCP3.. The mRNA levels of UCP3 were significantly different between the 2 feed conditions.

Article Title: Estrogen Receptors/E2F1/CDKN3 Axis Protects from UV-induced Skin Cancers in Females
Article Snippet: RNA quality was verified by microfluidic (Agilent 2100 Bioanalyzer) and concentration determined with a NanoDrop spectrophotometer (Wilmington). .. Total RNA (500ng – 1ug) was reverse transcribed using an RT Go script Kit (Promega) according to the manufacturer’s instructions. cDNA quantifications were performed with GoTaq qPCR Master Mix (Promega). ..

Article Title: Targeting NUPR1-dependent stress granules formation to induce synthetic lethality in Kras G12D -driven tumors
Article Snippet: Cells were treated with doxycycline or MARTX1133 30 nM for 24 h. The Oxygen Consumption Rate (OCR) (pmol O 2 /min) was measured using the Seahorse Bioscience XF24 Extracellular Flux Analyzer (Agilent) in response to 1 μM oligomycin, 0.25 μM carbonylcyanide p-(trifluoro-methoxy) phenylhydrazone (FCCP), 0.5 μM rotenone/Antimycine A (Millipore Sigma). .. Total RNA was extracted from cells using Trizol kit (Invitrogen) and cDNA was obtained by reverse-transcribed using Go Script kit (Promega), according to the manufacturer’s instructions. .. Real-time quantitative PCR (qRT-PCR) was performed using AriaMx system (Agilent).

Real-time Polymerase Chain Reaction:

Article Title: Estrogen Receptors/E2F1/CDKN3 Axis Protects from UV-induced Skin Cancers in Females
Article Snippet: RNA quality was verified by microfluidic (Agilent 2100 Bioanalyzer) and concentration determined with a NanoDrop spectrophotometer (Wilmington). .. Total RNA (500ng – 1ug) was reverse transcribed using an RT Go script Kit (Promega) according to the manufacturer’s instructions. cDNA quantifications were performed with GoTaq qPCR Master Mix (Promega). ..

other:

Article Title: Detection of emerging and re-emerging pathogens in surface waters close to an urban area.
Article Snippet: The q-PCR program as follows: initial step 45 °C for 900 s to perform the retro-transcription and 95 °C for 120 s for the inactivation of retro-transcriptase (Go Script, Promega, Madison , VL USA) and the activation of DNA polymerase (GoTaq®polymerase, Promega) followed by 45 cycles of: 95 °C for 15 s (denaturation) and 60 °C for 60 s (annealing and extension).

Article Title: The stress-induced protein NUPR1 orchestrates protein translation during ER-stress by interacting with eIF2α
Article Snippet: The RNAs were reverse-transcribed using GO Script kit (Promega) following manufacturer’s instruction.

Article Title: NUPR1 interacts with eIF2α and is required for resolution of the ER stress response in pancreatic tissue.
Article Snippet: The RNAs were reverse-transcribed using GO Script Kit (Promega, Madison, WI, USA) following manufacturer’s instruction.



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