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anti glua2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti glua2
    Anti Glua2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glua2/AMPA+Receptor+1+(GluA1)+Rabbit+mAb/pm41700129-55-40-41
    Average 96 stars, based on 331 article reviews
    anti glua2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: SNX6-mediated subunit-specific secretory trafficking of AMPA receptors regulates synaptic function and plasticity.
    Article Snippet: Adeno-associated virus (AAV) viral particles carrying pAOV-CaMKIIα-mCherry-2A-Cre, pAOV-CaMKIIα-EGFP-2A-Cre, pAAV-hSyn-EGFP-2A-SNX6-3Flag-tWPA and pAAV-hSyn-EGFP-2ASNX6-mut-3Flag-tWPA were purchased from OBiO Technology. .. The antibodies used in this study are as follows: mouse anti-GluA1 (Millipore, MAB2263; 1:100 for immunofluorescence (IF) and 1:1000 for Western blotting (WB)); mouse anti-GluA2 (Invitrogen, 32-0300; 1:100 for IF and 1:1000 for WB), rabbit anti-GluA1 (Cell Signaling Technology, 13185; 1:100 for IF), mouse anti-His (Cell Signaling Technology, 2366; 1:2000 for WB), rabbit anti-NMDAR2A (Rockland, 612- 401-D89; 1:1000 for WB), rabbit anti-SNX6 (Novus Biologicals, NBP2-13361; 1:100 for IF and 1:1000 for WB), rabbit anti-LAMP1 (Abcam, ab24170; 1:300 for IF), rabbit anti-Rab8 (Sigma-Aldrich, R5530; 1:100 for IF), rabbit anti-Flag (Sigma-Aldrich, F7425; 1:3000 for WB), rabbit anti-Rab11 (Thermo Fisher, 700184; 1:100 for IF), rabbit anti-Calreticulin (Thermo Fisher, MA532131; 1:1000 for WB), mouse antiGFP (MBL, M048-3; 1:500 for IF and 1:2000 for WB), rabbit anti-GFP (MBL, 598; 1:500 for IF and 1:2000 for WB), rabbit anti-RFP (MBL, PM005; 1:500 for IF and 1:2000 for WB), mouse anti-RFP (MBL, M1653; 1:500 for IF and 1:2000 for WB), rat anti-GFP (Santa Cruz Biotechnology, sc-101536; 1:3000 for WB), rabbit anti-Rab5B (Santa Cruz Biotechnology, sc-598; 1:100 for IF). ..

    Western Blot:

    Article Title: SNX6-mediated subunit-specific secretory trafficking of AMPA receptors regulates synaptic function and plasticity.
    Article Snippet: Adeno-associated virus (AAV) viral particles carrying pAOV-CaMKIIα-mCherry-2A-Cre, pAOV-CaMKIIα-EGFP-2A-Cre, pAAV-hSyn-EGFP-2A-SNX6-3Flag-tWPA and pAAV-hSyn-EGFP-2ASNX6-mut-3Flag-tWPA were purchased from OBiO Technology. .. The antibodies used in this study are as follows: mouse anti-GluA1 (Millipore, MAB2263; 1:100 for immunofluorescence (IF) and 1:1000 for Western blotting (WB)); mouse anti-GluA2 (Invitrogen, 32-0300; 1:100 for IF and 1:1000 for WB), rabbit anti-GluA1 (Cell Signaling Technology, 13185; 1:100 for IF), mouse anti-His (Cell Signaling Technology, 2366; 1:2000 for WB), rabbit anti-NMDAR2A (Rockland, 612- 401-D89; 1:1000 for WB), rabbit anti-SNX6 (Novus Biologicals, NBP2-13361; 1:100 for IF and 1:1000 for WB), rabbit anti-LAMP1 (Abcam, ab24170; 1:300 for IF), rabbit anti-Rab8 (Sigma-Aldrich, R5530; 1:100 for IF), rabbit anti-Flag (Sigma-Aldrich, F7425; 1:3000 for WB), rabbit anti-Rab11 (Thermo Fisher, 700184; 1:100 for IF), rabbit anti-Calreticulin (Thermo Fisher, MA532131; 1:1000 for WB), mouse antiGFP (MBL, M048-3; 1:500 for IF and 1:2000 for WB), rabbit anti-GFP (MBL, 598; 1:500 for IF and 1:2000 for WB), rabbit anti-RFP (MBL, PM005; 1:500 for IF and 1:2000 for WB), mouse anti-RFP (MBL, M1653; 1:500 for IF and 1:2000 for WB), rat anti-GFP (Santa Cruz Biotechnology, sc-101536; 1:3000 for WB), rabbit anti-Rab5B (Santa Cruz Biotechnology, sc-598; 1:100 for IF). ..

    Blocking Assay:

    Article Title: Disruption of hippocampal mitochondrial function underlies opioid-induced postoperative cognitive dysfunction in aged rats
    Article Snippet: Odyssey TBS blocking solution (Li-Cor) was used to prevent nonspecific protein binding (1 hr at RT). .. Primary antibodies, in Odyssey blocking solution containing 0.2% Tween20 (overnight at 4°C), were: SYP (1:1,000; sc-17750; Santa Cruz), PSD95 (1:1,000; 2507S; Cell Signaling), GluA1 (1:1000; 13185S; Cell Signaling) GluA2/3 (1:1000, 07-598; Millipore Sigma), GluN1 (1:500; MAB1586; Millipore Sigma), GluN2A (1:500; 07-632; Millipore Sigma) and GluN2B (1:1000; ab254356; Abcam), ..

    Article Title: Granulocyte colony-stimulating factor acts through calcium-permeable AMPA receptors to potentiate cocaine reward
    Article Snippet: Ten ug of protein were added to each well of a 4-12% polyacrylamide gel (Invitrogen) alongside a protein ladder (Bio-Rad, Precision Plus Dual Color) and electrophoresis was run at 100V until dye ran off the end of the gel. .. Proteins were transferred to a PVDF membrane and blocked with Intercept blocking buffer (LiCor) before incubation with GluR1 (1:1000, Cell Signaling Technology #13185), PSD95 (1:1000, Cell Signaling Technology #36233), or GluR2 (1:1000, Cell Signaling Technology #13607), and actin (1:5000, MP Biomedicals #691001) antibodies overnight at 4°C. ..

    Membrane:

    Article Title: Granulocyte colony-stimulating factor acts through calcium-permeable AMPA receptors to potentiate cocaine reward
    Article Snippet: Ten ug of protein were added to each well of a 4-12% polyacrylamide gel (Invitrogen) alongside a protein ladder (Bio-Rad, Precision Plus Dual Color) and electrophoresis was run at 100V until dye ran off the end of the gel. .. Proteins were transferred to a PVDF membrane and blocked with Intercept blocking buffer (LiCor) before incubation with GluR1 (1:1000, Cell Signaling Technology #13185), PSD95 (1:1000, Cell Signaling Technology #36233), or GluR2 (1:1000, Cell Signaling Technology #13607), and actin (1:5000, MP Biomedicals #691001) antibodies overnight at 4°C. ..

    Incubation:

    Article Title: Granulocyte colony-stimulating factor acts through calcium-permeable AMPA receptors to potentiate cocaine reward
    Article Snippet: Ten ug of protein were added to each well of a 4-12% polyacrylamide gel (Invitrogen) alongside a protein ladder (Bio-Rad, Precision Plus Dual Color) and electrophoresis was run at 100V until dye ran off the end of the gel. .. Proteins were transferred to a PVDF membrane and blocked with Intercept blocking buffer (LiCor) before incubation with GluR1 (1:1000, Cell Signaling Technology #13185), PSD95 (1:1000, Cell Signaling Technology #36233), or GluR2 (1:1000, Cell Signaling Technology #13607), and actin (1:5000, MP Biomedicals #691001) antibodies overnight at 4°C. ..



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