puast-derived plasmids mini white gene) containing gfp-smo coding sequences (Genetic Services Inc)
90
Structured Review
Genetic Services Inc
puast-derived plasmids mini white gene) containing gfp-smo coding sequences
Puast Derived Plasmids Mini White Gene) Containing Gfp Smo Coding Sequences, supplied by Genetic Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+coding+sequence/puast+derived+plasmids+mini+white+gene++containing+gfp+smo+coding+sequences/pmc00535705-118-13-32
Average 90 stars, based on 1 article reviews
Puast Derived Plasmids Mini White Gene) Containing Gfp Smo Coding Sequences, supplied by Genetic Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+coding+sequence/puast+derived+plasmids+mini+white+gene++containing+gfp+smo+coding+sequences/pmc00535705-118-13-32
Average 90 stars, based on 1 article reviews
puast-derived plasmids mini white gene) containing gfp-smo coding sequences - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "Extensive phosphorylation of Smoothened in Hedgehog pathway activation"
Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation
Journal:
doi: 10.1073/pnas.0408093101
Figure Legend Snippet: Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or pUAST-based plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using the prd-GAL4 driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
Techniques Used: In Vitro, In Vivo, Activity Assay, Expressing, Transfection, Reporter Assay, Over Expression, Immunofluorescence, Labeling, Variant Assay
Related Articles
Injection:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). In Vitro:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). In Vivo:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Activity Assay:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Expressing:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Transfection:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Reporter Assay:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Over Expression:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Immunofluorescence:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Labeling:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). Variant Assay:Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation Article Snippet: Approximately 24 h after HhN addition or 48 h after transfection with pAct5C GAL4 / UAS-hh , cell lysates were harvested and analyzed by SDS/PAGE and Western blotting by using anti-Myc rabbit polyclonal Ab (A-14; Santa Cruz Biotechnology), anti-V5 mouse mAb (Invitrogen), anti-tubulin mouse mAb E7 (Developmental Studies Hybridoma Bank, Iowa City, IA; contributed by M. Klymkowsky, University of Colorado, Boulder), and anti-lamin rabbit polyclonal Ab R836 (gift from P. Fisher, Stony Brook University, Stony Brook, NY). |