mouse igg santa cruz sc (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mouse igg santa cruz sc
Mouse Igg Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 4294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomics+suite+(software+analysis+microarray+data/normal+mouse+IgG/us11208656-812-178-180
Average 96 stars, based on 4294 article reviews

mouse igg santa cruz sc - by Bioz Stars, 2026-11

96/100 stars

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Negative Control:

Article Title: Pseudomonas aeruginosa Pyocyanin Increases Airway Mucin Biosynthesis by SIRT1-Mediated FOXA2 Inactivation.
Article Snippet: FOXA2 proteins were pulled-down by incubating with anti-FOXA2 antibody (SC-101060, Santa Cruz Biotech). .. Normal mouse IgG (sc-2025, Santa Cruz Biotech) was used as a negative control, which did not pull down FOXA2. .. To pull down HA-tagged FOXA2 wildtype and mutant recombinants, anti-HA antibody (SC-7392, Santa Cruz Biotech) was utilized.

Article Title: Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis.
Article Snippet: Article Nitrate-responsive Sialin2 couples stress granule assembly and clearance to maintain neuronal homeostasis

Pull Down Assay:

Chromatin Immunoprecipitation:

Article Title: CTCF-associated chromatin changes between HOXD1 and HOXD8 contribute to TNBC progression.
Article Snippet: .. Chromatin immunoprecipitation (ChIP)-grade anti-CTCF antibody (2899S, Cell Signaling Technology) and non-immune mouse IgG (sc2025, Santa Cruz) were used for immunoprecipitation. ..

Cell-Signaling:

Immunoprecipitation:

Control:

Article Title: OPTN-TRIP6-mediated F-actin mechanical support regulates phagophore closure to govern craniofacial skeletal development.
Article Snippet: The development of craniofacial bones is highly dependent on the intramembranous ossification process driven by orofacial mesenchymal stem cells (OMSCs).. Optineurin (OPTN), a traditional mitophagy receptor, promotes OMSC matrix mineralization by mediating the extracellular release of amorphous calcium phosphate (ACP) through the initiation of mitophagy via liquid-liquid phase separation.. However, the dynamic mechanisms by which OPTN regulates mitochondrial engulfment remain elusive.

Article Title: nHOTAIRM1 scaffolds ANXA11-dependent assemblies to drive axonal mRNA localization in human motor neurons.
Article Snippet: Cells (75 × 106) were UV crosslinked at 254 nm (4000 × 100 μJ/cm2) using a Stratalinker, lysed in NP-40 buffer (50mM Hepes-KOH pH 7.5, 150mM KCl, 2 mM EDTA, 1 mM NaF, 0.5% NP40, 0.5 mM DTT, 1× Protease Inhibitor Cocktail, RiboLock RNase Inhibitor (Thermo Fisher, cat#EO038SKB011)), and clarified by centrifugation (18,000 × g, 10 min, 4 °C). .. Lysates were incubated overnight at 4 °C with Dynabeads Protein G (Thermo Fisher, cat#10004D) pre-bound to antiANXA11 antibody (10 μg) or IgG control (Santa Cruz Biotechnologies, catsc-2025). .. Beads were washed with high-salt buffer (50mM Hepes-KOH, 500mM KCl, 0.5 mM DTT, 0.05% NP-40), treated with Proteinase K (Thermo Fisher, cat#AM2546, 20 mg/ml, 30min 50 °C), and RNA was extracted using miRNeasy Micro Kit (Qiagen) with DNase I.

Incubation:

Phalloidin Staining:

Article Title: Potential Role of the Urokinase-Type Plasminogen Activator Receptor-Integrin β3 Axis in Podocyte Adhesion Instability and Loss.
Article Snippet: .. Phalloidin Staining Differentiated human podocytes seeded onto the confocal dish were treated with integrinβ3 or IgG antibody (sc-2025, Santa Cruz; 1 μg/mL) for 30 minutes before applying FSS. .. Cells were then fixed with 4% paraformaldehyde in phosphate-buffered saline for 15 minutes at 37 ◦ C, followed by Alexa Fluor 594 phalloidin staining (1:40 dilution, A12381, Invitrogen, CA) for 20 minutes at room temperature in the dark.

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