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Genisphere llc array 900 kit
Array 900 Kit, supplied by Genisphere llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genisphere+array900+kit/array+900+kit/pmc01186732-254-10-14
Average 90 stars, based on 1 article reviews
array 900 kit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida -challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons
Article Snippet: To protect dyes from fading, slides were dipped for 5 s in Dye SaverTM 2 anti-fade coating (Genisphere 900TM Hatfield, PA) followed by centrifugation.

Article Title: Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus
Article Snippet: Films were densitometrically analyzed using NIH ImageJ (version 1.62f).

Article Title: Methods and kits for linking polymorphic sequences to expanded repeat mutations
Article Snippet: Examples include the SuperscriptTM Double Strand cDNA Synthesis Kit (Invitrogen, Carlsbad, Calif.), the Array 50TM, Array 350TM and Array 900TM Detection Kits (Genisphere, Hatfield, Pa.), and the CyScribeTM Post-Labelling Kit (Amersham, Piscataway, N.J.).

Isolation:

Article Title: Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus
Article Snippet: .. Total RNA from early-passage rheumatoid synovial fibroblasts (3 early-passage lines per experimental group) was isolated using RNeasy miniprep columns (Qiagen), and labeling and hybridization were performed with the Genisphere Array 900 Expression Array Detection Kit according to the manufacturer’s protocol. .. The OMM25K oligonucleotide gene array set (18,000 genes) was used ( http://support.illumina.com/array/array_kits/humanht-12_v4_expression_beadchip_kit.html ) and the cDNA probe and the fluorescent 3DNA reagent were hybridized to the microarray in succession using an Advalytix Slide Booster hybridization station.

Labeling:

Article Title: Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus
Article Snippet: .. Total RNA from early-passage rheumatoid synovial fibroblasts (3 early-passage lines per experimental group) was isolated using RNeasy miniprep columns (Qiagen), and labeling and hybridization were performed with the Genisphere Array 900 Expression Array Detection Kit according to the manufacturer’s protocol. .. The OMM25K oligonucleotide gene array set (18,000 genes) was used ( http://support.illumina.com/array/array_kits/humanht-12_v4_expression_beadchip_kit.html ) and the cDNA probe and the fluorescent 3DNA reagent were hybridized to the microarray in succession using an Advalytix Slide Booster hybridization station.

Article Title: Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida -challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons
Article Snippet: .. Total RNA (25 μg) was reverse transcribed with 200 units of Superscript III (Invitrogen) with primer tails that complemented Cy3 or Cy5 labeled flurophores (Genisphere 900TM). .. In each reaction, 1 μL of Alien oligos® (Stratagene, La Jolla, CA) was used as a positive control and to facilitated optimization of scanning parameters.

Article Title: Biological systems input-output response system and plant sentinels
Article Snippet: Total RNA was isolated from each sample (8 plants/treatment) using AurumTM Total RNA Mini kit (Bio-Rad Laboratories, Hercules, Calif.). .. Paired 2.5 μg aliquots from each treated and control RNA sample were reciprocally labeled with either Cy3 or Cy5 dendrimers using the Array 900TM system (Genisphere, Hatfield, Pa.), such that RNA from each treatment was labeled and hybridized (in parallel with a contrastingly labeled control sample) four separate times, for a total of twelve microarrays balanced with respect to treatment, dye, and biological replication. ..

Hybridization:

Article Title: Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus
Article Snippet: .. Total RNA from early-passage rheumatoid synovial fibroblasts (3 early-passage lines per experimental group) was isolated using RNeasy miniprep columns (Qiagen), and labeling and hybridization were performed with the Genisphere Array 900 Expression Array Detection Kit according to the manufacturer’s protocol. .. The OMM25K oligonucleotide gene array set (18,000 genes) was used ( http://support.illumina.com/array/array_kits/humanht-12_v4_expression_beadchip_kit.html ) and the cDNA probe and the fluorescent 3DNA reagent were hybridized to the microarray in succession using an Advalytix Slide Booster hybridization station.

Article Title: Microarray analysis of the in vivo sequence preferences of a minor groove binding drug
Article Snippet: Total RNA was isolated from the two yeast cultures using the Ambion Ribopure Yeast kit and tested for quality with both UV absorbance and agarose gel electrophoresis. .. Using the Genisphere Array 900 kit, we prepared cDNA from mRNA for the two RNA populations and conducted a two step hybridization involving Cy3 and Cy5 fluorophores to a DNA microarray spotted with 70 mer oligos that represent the known reading frames in the yeast genome. ..

Expressing:

Article Title: Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus
Article Snippet: .. Total RNA from early-passage rheumatoid synovial fibroblasts (3 early-passage lines per experimental group) was isolated using RNeasy miniprep columns (Qiagen), and labeling and hybridization were performed with the Genisphere Array 900 Expression Array Detection Kit according to the manufacturer’s protocol. .. The OMM25K oligonucleotide gene array set (18,000 genes) was used ( http://support.illumina.com/array/array_kits/humanht-12_v4_expression_beadchip_kit.html ) and the cDNA probe and the fluorescent 3DNA reagent were hybridized to the microarray in succession using an Advalytix Slide Booster hybridization station.

Reverse Transcription:

Article Title: Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida -challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons
Article Snippet: .. Total RNA (25 μg) was reverse transcribed with 200 units of Superscript III (Invitrogen) with primer tails that complemented Cy3 or Cy5 labeled flurophores (Genisphere 900TM). .. In each reaction, 1 μL of Alien oligos® (Stratagene, La Jolla, CA) was used as a positive control and to facilitated optimization of scanning parameters.

Control:

Article Title: Biological systems input-output response system and plant sentinels
Article Snippet: Total RNA was isolated from each sample (8 plants/treatment) using AurumTM Total RNA Mini kit (Bio-Rad Laboratories, Hercules, Calif.). .. Paired 2.5 μg aliquots from each treated and control RNA sample were reciprocally labeled with either Cy3 or Cy5 dendrimers using the Array 900TM system (Genisphere, Hatfield, Pa.), such that RNA from each treatment was labeled and hybridized (in parallel with a contrastingly labeled control sample) four separate times, for a total of twelve microarrays balanced with respect to treatment, dye, and biological replication. ..

Microarray:

Article Title: Microarray analysis of the in vivo sequence preferences of a minor groove binding drug
Article Snippet: Total RNA was isolated from the two yeast cultures using the Ambion Ribopure Yeast kit and tested for quality with both UV absorbance and agarose gel electrophoresis. .. Using the Genisphere Array 900 kit, we prepared cDNA from mRNA for the two RNA populations and conducted a two step hybridization involving Cy3 and Cy5 fluorophores to a DNA microarray spotted with 70 mer oligos that represent the known reading frames in the yeast genome. ..



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Genisphere llc genisphere array900 kit
Genisphere Array900 Kit, supplied by Genisphere llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genisphere+array900+kit/array900+detection+kit/pmc03066294-135-7-9
Average 90 stars, based on 1 article reviews
genisphere array900 kit - by Bioz Stars, 2026-10
90/100 stars
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