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genesifter microarray analysis system  (VizX Labs)

 
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    VizX Labs genesifter microarray analysis system
    Genesifter Microarray Analysis System, supplied by VizX Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genesifter+microarray+data+analysis+system/genesifter+microarray+data+analysis+system/pmc03621729-177-6-7
    Average 90 stars, based on 1 article reviews
    genesifter microarray analysis system - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors
    Article Snippet: Data was analyzed with Affymetrix Micro array Suite 5.0, and GeneSifter web-based microarray analysis system (VizX Labs, Seattle, WA).

    Microarray:

    Article Title: Modulation of the anti-cancer efficacy of microtubule-targeting agents by cellular growth conditions
    Article Snippet: .. Data was processed with the GeneSifter (VizX Labs) microarray analysis system. ..

    Article Title: Modulation of the anti-cancer efficacy of microtubule-targeting agents by cellular growth conditions
    Article Snippet: .. Microarray data was processed with the GeneSifter (VizX Labs) microarray analysis system. .. We screened the data by eliminating genes that changed by less than 2-fold, and by only including genes that were flagged as “P” or “present” by the Affymetrix MAS5 algorithm.

    Article Title: Disseminated and Rapidly Fatal Tuberculosis in Mice Bearing a Defective Allele AT IRF-8
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (Day 30 and 70) groups for B6 mice. ..

    Article Title: Human pluripotent embryonic stem cells produced by nuclear transfer using a somatic cell nucleus treated with HVJ-E extract and an oocyte from a donor cycle that produced 15 or fewer oocytes
    Article Snippet: .. GeneSifter® (VizX Labs, Seattle, Wash.) microarray expression analysis software was used to identify differentially expressed transcripts. ..

    Article Title: GENETIC AND FUNCTIONAL CHARACTERIZATION OF THE MOUSE TRL3 LOCUS IN DEFENSE AGAINST TUBERCULOSIS
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (day 30 and 70) groups for B6, D2, D2.B6-Chr7 and D2.B6-Chr19 mice individually. ..

    Generated:

    Article Title: Disseminated and Rapidly Fatal Tuberculosis in Mice Bearing a Defective Allele AT IRF-8
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (Day 30 and 70) groups for B6 mice. ..

    Article Title: GENETIC AND FUNCTIONAL CHARACTERIZATION OF THE MOUSE TRL3 LOCUS IN DEFENSE AGAINST TUBERCULOSIS
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (day 30 and 70) groups for B6, D2, D2.B6-Chr7 and D2.B6-Chr19 mice individually. ..

    Control:

    Article Title: Disseminated and Rapidly Fatal Tuberculosis in Mice Bearing a Defective Allele AT IRF-8
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (Day 30 and 70) groups for B6 mice. ..

    Article Title: GENETIC AND FUNCTIONAL CHARACTERIZATION OF THE MOUSE TRL3 LOCUS IN DEFENSE AGAINST TUBERCULOSIS
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (day 30 and 70) groups for B6, D2, D2.B6-Chr7 and D2.B6-Chr19 mice individually. ..

    Infection:

    Article Title: Disseminated and Rapidly Fatal Tuberculosis in Mice Bearing a Defective Allele AT IRF-8
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (Day 30 and 70) groups for B6 mice. ..

    Article Title: GENETIC AND FUNCTIONAL CHARACTERIZATION OF THE MOUSE TRL3 LOCUS IN DEFENSE AGAINST TUBERCULOSIS
    Article Snippet: .. The GeneSifter TM microarray data analysis system (VizX Labs, Seattle, WA, USA; www.genesifter.net ) was used to analyze data generated from comparisons between control (uninfected) and M. tuberculosis -infected (day 30 and 70) groups for B6, D2, D2.B6-Chr7 and D2.B6-Chr19 mice individually. ..

    Expressing:

    Article Title: Human pluripotent embryonic stem cells produced by nuclear transfer using a somatic cell nucleus treated with HVJ-E extract and an oocyte from a donor cycle that produced 15 or fewer oocytes
    Article Snippet: .. GeneSifter® (VizX Labs, Seattle, Wash.) microarray expression analysis software was used to identify differentially expressed transcripts. ..

    Software:

    Article Title: Human pluripotent embryonic stem cells produced by nuclear transfer using a somatic cell nucleus treated with HVJ-E extract and an oocyte from a donor cycle that produced 15 or fewer oocytes
    Article Snippet: .. GeneSifter® (VizX Labs, Seattle, Wash.) microarray expression analysis software was used to identify differentially expressed transcripts. ..



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    BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
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    BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
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    BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
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    BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding microarray results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.

    Journal: PLoS Genetics

    Article Title: Interferon Regulatory Factor 8 Regulates Pathways for Antigen Presentation in Myeloid Cells and during Tuberculosis

    doi: 10.1371/journal.pgen.1002097

    Figure Lengend Snippet: BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding microarray results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.

    Article Snippet: The GeneSifter™ microarray data analysis system (Geospiza Inc., Seattle, WA, USA) was used to examine data generated from comparisons between control (unstimulated) and IFNγ/CpG-stimulated (3 hrs) groups.

    Techniques: Mutagenesis, Expressing, Functional Assay, Microarray