Review




Structured Review

BioCore Inc microarray analysis
(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using <t>microarray</t> data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).
Microarray Analysis, supplied by BioCore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/microarray+data+analysis/pmc06743866-53-1-16
Average 90 stars, based on 1 article reviews
microarray analysis - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation"

Article Title: Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0222559

(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).
Figure Legend Snippet: (A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P -values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade ( Ccl2 , Ccl7 , Ccl8 , C3 , and Pdgfa ). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).

Techniques Used: Modification, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, SYBR Green Assay

Related Articles

Microarray:

Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer
Article Snippet: .. Colorectal cancer tissue microarray (CRC TMA) A formalin-fixed paraffin-embedded (FFPE) CRC TMA was developed by the University of Wisconsin Carbone Cancer Center Translational Science Biocore (TSB) under an IRB-approved protocol (2016–0934) as previously described [ 15 ]. ..

Article Title: Investigating miR-6880-5p in extracellular vesicle from plasma as a prognostic biomarker in endocrine therapy-treated castration-resistant prostate cancer.
Article Snippet: .. The isolated total EV-RNAs were sent for miRNA microarray to Biocore (Seoul, Republic of Korea). .. RNA quality was assessed using the agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, California, USA).

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer
Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Affymetrix expression microarray analysis of the Hs578T cell line was performed by BioCore. ..

Article Title: Circulating microRNA-423 attenuates the phosphorylation of calcium handling proteins in atrial fibrillation
Article Snippet: Image processing was performed using an Affymetrix Gene Array 3000 scanner, and data analysis was performed using GeneSpring GX 14.9.1 software. .. Quality control and microarray analysis of small RNA experiments were performed at Biocore (Seoul, Korea). ..

Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer.
Article Snippet: Department of Oncology, University of Wisconsin, Madison, WI, 53705, USA Carbone Cancer Center, University of Wisconsin, Madison, WI, 53705, USA Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, 53705, USA William S. Middleton VA Medical Center, Madison, WI, 53705, USA Department of Statistics, University of Wisconsin, Madison, WI, 53706, USA Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, 53792, USA Division of Hematology, Medical Oncology, and Palliative Care, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Department of Internal Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA Department of Radiology, University of Wisconsin, Madison, WI, 53705, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA

Article Title: Stromal Versican Accumulation and Proteolysis Regulate the Infiltration of CD8 + T Cells in Breast Cancer
Article Snippet: .. A total of 371 cases of invasive breast cancer were identified and used to generate a tissue microarray (TMA) that was obtained through the UW–Madison Translational Science Biocore [ ]. ..

Formalin-fixed Paraffin-Embedded:

Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer
Article Snippet: .. Colorectal cancer tissue microarray (CRC TMA) A formalin-fixed paraffin-embedded (FFPE) CRC TMA was developed by the University of Wisconsin Carbone Cancer Center Translational Science Biocore (TSB) under an IRB-approved protocol (2016–0934) as previously described [ 15 ]. ..

Article Title: Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer.
Article Snippet: Department of Oncology, University of Wisconsin, Madison, WI, 53705, USA Carbone Cancer Center, University of Wisconsin, Madison, WI, 53705, USA Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, 53705, USA William S. Middleton VA Medical Center, Madison, WI, 53705, USA Department of Statistics, University of Wisconsin, Madison, WI, 53706, USA Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, 53792, USA Division of Hematology, Medical Oncology, and Palliative Care, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA Department of Internal Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA Department of Radiology, University of Wisconsin, Madison, WI, 53705, USA Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin, Madison, WI, 53705, USA

other:

Article Title: Mesenchymal stem cell-derived exosomes suppress proliferation of T cells by inducing cell cycle arrest through p27kip1/Cdk2 signaling.
Article Snippet: Mouse mesenchymal stem cells (MSCs) have been shown to suppress T cells.. Especially, MSC-cultured media have shown suppressive functions against various immune cells including the T cells.. However, the underlying immunosuppressive mechanisms of the MSC-cultured medium are not yet fully understood.

Isolation:

Article Title: Investigating miR-6880-5p in extracellular vesicle from plasma as a prognostic biomarker in endocrine therapy-treated castration-resistant prostate cancer.
Article Snippet: .. The isolated total EV-RNAs were sent for miRNA microarray to Biocore (Seoul, Republic of Korea). .. RNA quality was assessed using the agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, California, USA).

Multiple Displacement Amplification:

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer
Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Affymetrix expression microarray analysis of the Hs578T cell line was performed by BioCore. ..

Expressing:

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer
Article Snippet: For CD107a and IFNγ staining, cells were treated with brefeldin A (BioLegend, 420601) for 5 h before incubation with the primary antibody. .. Microarray data of MDA-MB231 cells were downloaded from the GEO database (GSE83325).37 Affymetrix expression microarray analysis of the Hs578T cell line was performed by BioCore. ..

Control:

Article Title: Circulating microRNA-423 attenuates the phosphorylation of calcium handling proteins in atrial fibrillation
Article Snippet: Image processing was performed using an Affymetrix Gene Array 3000 scanner, and data analysis was performed using GeneSpring GX 14.9.1 software. .. Quality control and microarray analysis of small RNA experiments were performed at Biocore (Seoul, Korea). ..

Tissue:

Article Title: Stromal Versican Accumulation and Proteolysis Regulate the Infiltration of CD8 + T Cells in Breast Cancer.
Article Snippet: .. A total of 371 cases of invasive breast cancer were identified and used to generate a tissue microarray (TMA) that was obtained through the UW–Madison Translational Science Biocore [22]. ..



Similar Products

90
VizX Labs genesifter tm microarray data analysis system
Genesifter Tm Microarray Data Analysis System, supplied by VizX Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/genesifter+microarray+data+analysis+system/pmc04307599-67-1-7
Average 90 stars, based on 1 article reviews
genesifter tm microarray data analysis system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
VizX Labs genesifter ™ microarray data analysis system
Genesifter ™ Microarray Data Analysis System, supplied by VizX Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/genesifter+microarray+data+analysis+system/pmc04307599-108-1-7
Average 90 stars, based on 1 article reviews
genesifter ™ microarray data analysis system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Geospiza Inc genesifter™ microarray data analysis system
BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
Genesifter™ Microarray Data Analysis System, supplied by Geospiza Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/genesifter++analysis+edition/pmc03121741-212-1-6
Average 90 stars, based on 1 article reviews
genesifter™ microarray data analysis system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
VizX Labs genesifter microarray data analysis system
BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
Genesifter Microarray Data Analysis System, supplied by VizX Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/genesifter+microarray+data+analysis+system/pmc02856624-69-12-20
Average 90 stars, based on 1 article reviews
genesifter microarray data analysis system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
VizX Labs the genesifter microarray data analysis system
BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding <t>microarray</t> results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.
The Genesifter Microarray Data Analysis System, supplied by VizX Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genesifter%E2%84%A2+microarray+data+analysis+system/genesifter+microarray+data+analysis+system/pmc02654745-164-10-15
Average 90 stars, based on 1 article reviews
the genesifter microarray data analysis system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding microarray results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.

Journal: PLoS Genetics

Article Title: Interferon Regulatory Factor 8 Regulates Pathways for Antigen Presentation in Myeloid Cells and during Tuberculosis

doi: 10.1371/journal.pgen.1002097

Figure Lengend Snippet: BMDMs RNA was obtained from individual wt and IRF8 mutant F2 mice either prior to (unstimulated control) or 3 hrs following stimulation with IFNγ/CpG (6 samples per experimental group; 24 samples in total), and hybridized to microarrays. (A) By using a 2×2 interaction Anova analysis, 368 genes were recognized to be significantly differentially modulated by IRF8 in response to IFNγ/CpG exposure between the wt and IRF8 mutant cells. The expression profiles are ordered by hierarchical clustering; the genes, illustrated by their specific signal intensities (Log 2 scale) are displayed as rows and individual mouse samples/conditions as columns. Red coloring signifies high level of expression; green coloring denotes low level of expression. The dendrogram illustrates the clustering of the samples according to expression pattern similarities. RNA samples (6 per experimental group) used for this transcriptional profiling analysis were pooled and used for qPCR validation. Ephx1 , Cyp27a1 , Ciita , and Il10ra were selected as genes positively affected by the presence of functional IRF8 following IFNγ/CpG exposure (B), while Ms4a7 , C1qb , Angptl4 , and Slc40a1 were selected as genes negatively affected by the presence of a functional IRF8 following IFNγ/CpG exposure (C). The ratios of expression (IFNγ/CpG-stimulated versus unstimulated control), represented by fold induction, were calculated for the wt and IRF8 mutant mice separately (white bars), and compared to the corresponding microarray results (black bars). The black dots indicate the qPCR values obtained for each replicate. The microarray results were statistically significant according to Anova analysis ( t test p value of 0.05 and a fold-change cutoff of 1.5X). Hprt was used to standardize the mRNA levels of target genes for qPCR.

Article Snippet: The GeneSifter™ microarray data analysis system (Geospiza Inc., Seattle, WA, USA) was used to examine data generated from comparisons between control (unstimulated) and IFNγ/CpG-stimulated (3 hrs) groups.

Techniques: Mutagenesis, Expressing, Functional Assay, Microarray