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Incyte corporation expression microarray
Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same <t>microarray.</t> (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
Expression Microarray, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "How Can Microarrays Unlock Asthma?"

Article Title: How Can Microarrays Unlock Asthma?

Journal: Journal of Allergy

doi: 10.1155/2012/241314

Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
Figure Legend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Techniques Used: Expressing, Derivative Assay, Labeling, Reverse Transcription, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

The GEO accession number for  microarray  studies conducted on asthma.
Figure Legend Snippet: The GEO accession number for microarray studies conducted on asthma.

Techniques Used: Microarray, Gene Expression, Clone Assay, Expressing, Virus, Infection, Functional Assay, Sequencing, Synthesized, Comparison

Related Articles

Expressing:

Article Title: Regulation of c-Met-dependent gene expression by PTEN.
Article Snippet: .. SF/HGF alters the expression of many more genes in glioblastoma cells than in normal astrocytes We used Incyte gene microarrays to compare the effects of SF/HGF on gene expression profiles in NHA and U-373MG human glioblastoma cells. ..

Article Title: How Can Microarrays Unlock Asthma?
Article Snippet: .. In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an expression microarray containing 8159 human gene cDNA clones from Research Genetics (IMAGE consortium, Huntsville, AL), Incyte Genomics (Santa Clara, CA) [ ]. ..

Gene Expression:

Article Title: Regulation of c-Met-dependent gene expression by PTEN.
Article Snippet: .. SF/HGF alters the expression of many more genes in glioblastoma cells than in normal astrocytes We used Incyte gene microarrays to compare the effects of SF/HGF on gene expression profiles in NHA and U-373MG human glioblastoma cells. ..

Article Title: Identification and function of hypoxia-response genes in Drosophila melanogaster.
Article Snippet: .. A gene expression microarray with 21,000 spots ( 16,000 spots from PCRs of predicted open reading frames from Incyte Genomics and 5,000 from DGC cDNA clones from BDGP) were printed on poly-L-lysine-coated slides and after postprocessing were used for hybridization as in Ref. 1. ..

Article Title: Targeting Tomoregulin for Radioimmunotherapy of Prostate Cancer
Article Snippet: Radiotherapy is an effective approach for the treatment of local prostate cancer.. However, once prostate cancer metastasizes, radiotherapy cannot be used due to the distribution of multiple metastases to lymph nodes and bones.. In contrast, radioimmunotherapy should still be efficacious in metastatic prostate cancer as radioisotopes are brought to tumor cells by targeting antibodies.

Article Title: SC6 for diagnosis of cancers
Article Snippet: .. Gene Expression Microarray (GEM) Analysis A detailed analysis of differential gene expression in HDMEC cells grown under normoxic (control) or hypoxic (treated) conditions for 4, 8 and 16 h was performed using the GEMTM microarray services offered by Incyte Genomics, Ltd, Paloaoto, USA). ..

Microarray:

Article Title: Identification and function of hypoxia-response genes in Drosophila melanogaster.
Article Snippet: .. A gene expression microarray with 21,000 spots ( 16,000 spots from PCRs of predicted open reading frames from Incyte Genomics and 5,000 from DGC cDNA clones from BDGP) were printed on poly-L-lysine-coated slides and after postprocessing were used for hybridization as in Ref. 1. ..

Article Title: How Can Microarrays Unlock Asthma?
Article Snippet: .. In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an expression microarray containing 8159 human gene cDNA clones from Research Genetics (IMAGE consortium, Huntsville, AL), Incyte Genomics (Santa Clara, CA) [ ]. ..

Article Title: SC6 for diagnosis of cancers
Article Snippet: .. Gene Expression Microarray (GEM) Analysis A detailed analysis of differential gene expression in HDMEC cells grown under normoxic (control) or hypoxic (treated) conditions for 4, 8 and 16 h was performed using the GEMTM microarray services offered by Incyte Genomics, Ltd, Paloaoto, USA). ..

Article Title: Progressions on the Coexistence of Neuronal and Glial Precursor Cells in the Cerebral Ventricular Zone
Article Snippet: .. When I moved to the University of Pittsburgh, I had already been considering the new gene microarray technologies that were early in production by Affimetrix and Incyte for application to our developmental studies of molecular heterogeneity. ..

Clone Assay:

Article Title: Identification and function of hypoxia-response genes in Drosophila melanogaster.
Article Snippet: .. A gene expression microarray with 21,000 spots ( 16,000 spots from PCRs of predicted open reading frames from Incyte Genomics and 5,000 from DGC cDNA clones from BDGP) were printed on poly-L-lysine-coated slides and after postprocessing were used for hybridization as in Ref. 1. ..

Article Title: How Can Microarrays Unlock Asthma?
Article Snippet: .. In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an expression microarray containing 8159 human gene cDNA clones from Research Genetics (IMAGE consortium, Huntsville, AL), Incyte Genomics (Santa Clara, CA) [ ]. ..

Hybridization:

Article Title: Identification and function of hypoxia-response genes in Drosophila melanogaster.
Article Snippet: .. A gene expression microarray with 21,000 spots ( 16,000 spots from PCRs of predicted open reading frames from Incyte Genomics and 5,000 from DGC cDNA clones from BDGP) were printed on poly-L-lysine-coated slides and after postprocessing were used for hybridization as in Ref. 1. ..

other:

Article Title: Fluorescent oligonucleotides and uses thereof
Article Snippet: Microarray-based Gene Expression Assays Custom Gene Expression Microarrays (GEMs) with approximately 10000 elements (cDNA clones from either corn or soybean) were fabricated and provided by Incyte Microarray Division (Fremont.

Article Title: Transcriptional analysis and functional genomics in wheat
Article Snippet: In the post-genomic era the development of tools such as transcription microarrays allows the investigation of the molecular basis and regulation of plant growth, development and physiology in ever increasing detail.. Systems such as the ‘model’ plant species Arabidopsis offer the opportunity to develop generalised models for complex genetic regulatory networks.. However, researchers will need to apply tools to both monitor and alter gene expression in crop plants such as wheat to produce sophisticated and predictive models of development and environmental interactions.

Control:

Article Title: SC6 for diagnosis of cancers
Article Snippet: .. Gene Expression Microarray (GEM) Analysis A detailed analysis of differential gene expression in HDMEC cells grown under normoxic (control) or hypoxic (treated) conditions for 4, 8 and 16 h was performed using the GEMTM microarray services offered by Incyte Genomics, Ltd, Paloaoto, USA). ..



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Image Search Results


ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Journal: International Journal of Medical Sciences

Article Title: Long noncoding RNA ZEB1-AS1 acts as a Sponge of miR-141-3p to Inhibit Cell Proliferation in Colorectal Cancer

doi: 10.7150/ijms.46698

Figure Lengend Snippet: ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Article Snippet: Shanghai Biotechnology Co., Ltd conducted the microRNA microarray gene expression experiments and data analysis.

Techniques: Fractionation, Expressing, Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Assay, Activity Assay

ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Journal: Journal of Cancer

Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2

doi: 10.7150/jca.34886

Figure Lengend Snippet: ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Article Snippet: TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service.

Techniques: Microarray, Expressing, Control, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Functional Assay, Activity Assay, Fluorescence, Flow Cytometry