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CEM Corporation human exon microarray analysis
Human Exon Microarray Analysis, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+microarray+experiments/gene+expression+microarray+analysis/pmc08877913-312-21-20
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Microarray:

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. The microarray data obtained in the present study are available in the following database. (1) Human exon microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193574) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193574 ; (2) Human gene expression microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193576) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193576 . ..

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEMControl), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Data Availability Statement: The microarray data obtained in the present study are available in the following database. (1) Human exon microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193574) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193574; (2) Human gene expression microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193576) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193576. ..

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEM-Control), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State
Article Snippet: .. The 400 most-IFN- -inducible ISGs were chosen using a compilation of microarray data from 293T, HT1080, CEM, Jurkat, MT2, MT4, C8166, Hut R5, H9, Sup T1, U937, THP-1, K562, HL60, and KG1a cells (Table 1). ..

Gene Expression:

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. The microarray data obtained in the present study are available in the following database. (1) Human exon microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193574) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193574 ; (2) Human gene expression microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193576) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193576 . ..

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEMControl), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Data Availability Statement: The microarray data obtained in the present study are available in the following database. (1) Human exon microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193574) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193574; (2) Human gene expression microarray analysis in CEM cells overexpressing HTLV-1 Rex GEO (GSE193576) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193576. ..

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEM-Control), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight
Article Snippet: .. Gene expression data from the NASA Twins study was provided by Cem Meydan in the Mason Lab in csv format and organized by Dr. Afshin Beheshti as normalized mean counts for four immune cell types. ..

other:

Article Title: Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA.
Article Snippet: List of gene expression from genome wide analysis in CCRF-CEM cells. (XLSX 44303 kb) Additional file 4: Figure S2.

Expressing:

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEMControl), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEM-Control), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Functional Assay:

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEMControl), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.

Article Title: Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
Article Snippet: .. Briefly, the whole dataset of gene expression microarray analysis, i.e., data of all probes with expression levels (CEM-Rex against CEM-Control), were input to “Protein with Value/Ranks” application of STRING (v11.5) to perform the Functional Enrichment Analysis (FEA, hereafter). .. The results were returned as lists of terms in various databases such as Gene Ontology, KEGG, Pathways, Reactome, COMPARTMENTS, UniProt, Pfam, and InterPro, which are significantly enriched with our gene expression data.



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ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The <t>microRNA</t> array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.
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ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.
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ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Journal: International Journal of Medical Sciences

Article Title: Long noncoding RNA ZEB1-AS1 acts as a Sponge of miR-141-3p to Inhibit Cell Proliferation in Colorectal Cancer

doi: 10.7150/ijms.46698

Figure Lengend Snippet: ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Article Snippet: Shanghai Biotechnology Co., Ltd conducted the microRNA microarray gene expression experiments and data analysis.

Techniques: Fractionation, Expressing, Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Assay, Activity Assay

ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Journal: Journal of Cancer

Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2

doi: 10.7150/jca.34886

Figure Lengend Snippet: ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Article Snippet: TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service.

Techniques: Microarray, Expressing, Control, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Functional Assay, Activity Assay, Fluorescence, Flow Cytometry