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Genotypic Technology Pvt Ltd whole-genome gene-expression microarray
Uptake and transport of silver in C. <t>reinhardtii.</t> Intracellular concentrations of silver (A) on exposure to 100 and 200 nM, and (Inset) to 500-nM exposure. No intracellular silver was quantifiable in C. reinhardtii exposed to 10 nM silver and is therefore not shown in the graph. Heat map of Cu transporters (B) in C. reinhardtii exposed to silver for varying durations with each box representing a protein at the transcriptome and proteome level, green being down-regulated and red up-regulated. The SD is shown in the figures. Atx1, antioxidant 1, copper chaperone; Cox2A, subunit 2A of cytochrome oxidase; Ctr1 and -3, copper transporter 1 and 3; Fox1, Ferroxidase; Pcy1, Plastocyanin. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).
Whole Genome Gene Expression Microarray, supplied by Genotypic Technology Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1319388111

Uptake and transport of silver in C. reinhardtii. Intracellular concentrations of silver (A) on exposure to 100 and 200 nM, and (Inset) to 500-nM exposure. No intracellular silver was quantifiable in C. reinhardtii exposed to 10 nM silver and is therefore not shown in the graph. Heat map of Cu transporters (B) in C. reinhardtii exposed to silver for varying durations with each box representing a protein at the transcriptome and proteome level, green being down-regulated and red up-regulated. The SD is shown in the figures. Atx1, antioxidant 1, copper chaperone; Cox2A, subunit 2A of cytochrome oxidase; Ctr1 and -3, copper transporter 1 and 3; Fox1, Ferroxidase; Pcy1, Plastocyanin. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).
Figure Legend Snippet: Uptake and transport of silver in C. reinhardtii. Intracellular concentrations of silver (A) on exposure to 100 and 200 nM, and (Inset) to 500-nM exposure. No intracellular silver was quantifiable in C. reinhardtii exposed to 10 nM silver and is therefore not shown in the graph. Heat map of Cu transporters (B) in C. reinhardtii exposed to silver for varying durations with each box representing a protein at the transcriptome and proteome level, green being down-regulated and red up-regulated. The SD is shown in the figures. Atx1, antioxidant 1, copper chaperone; Cox2A, subunit 2A of cytochrome oxidase; Ctr1 and -3, copper transporter 1 and 3; Fox1, Ferroxidase; Pcy1, Plastocyanin. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Techniques Used: Algae

Regulation of functional pathways in C. reinhardtii at physiological (A, C, E, G) and molecular (B, D, F, H) levels. Photosynthesis (A, B); lipid peroxidation compared with the control (C) and oxidative stress response (D); growth (E, F); ATP content (G) and synthesis (H). In the heat maps each square represents a protein, with green being down-regulated and red up-regulated. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).
Figure Legend Snippet: Regulation of functional pathways in C. reinhardtii at physiological (A, C, E, G) and molecular (B, D, F, H) levels. Photosynthesis (A, B); lipid peroxidation compared with the control (C) and oxidative stress response (D); growth (E, F); ATP content (G) and synthesis (H). In the heat maps each square represents a protein, with green being down-regulated and red up-regulated. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Techniques Used: Functional Assay, Control, Algae

Molecular and physiological changes of lipid synthesis in C. reinhardtii exposed to silver for 1 h. Regulation of proteins (A) and lipid bodies containing precursors of lipids stained green with Nile red (B). The chloroplasts autoflourescence is seen as red. (Scale bars in B, 10 µm.) For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).
Figure Legend Snippet: Molecular and physiological changes of lipid synthesis in C. reinhardtii exposed to silver for 1 h. Regulation of proteins (A) and lipid bodies containing precursors of lipids stained green with Nile red (B). The chloroplasts autoflourescence is seen as red. (Scale bars in B, 10 µm.) For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Techniques Used: Staining, Algae

The toxicity and adaptive response pathways, as derived from linking transcriptome and proteome responses to physiological effects. (A) Schematic representation of biological pathways in C. reinhardtii affected by Ag+. (B) Schematic representation of the toxicity pathway. (C) Schematic representation of the adaptive-response pathway.
Figure Legend Snippet: The toxicity and adaptive response pathways, as derived from linking transcriptome and proteome responses to physiological effects. (A) Schematic representation of biological pathways in C. reinhardtii affected by Ag+. (B) Schematic representation of the toxicity pathway. (C) Schematic representation of the adaptive-response pathway.

Techniques Used: Derivative Assay

Related Articles

Microarray:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Algae:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Functional Assay:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Control:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Staining:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Derivative Assay:

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.



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