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gc tissue microarray chip  (Proteintech)


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    Proteintech gc tissue microarray chip
    Gc Tissue Microarray Chip, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+tissue+microarray/STUB1+Antibody/pmc12436174-80-5-32
    Average 96 stars, based on 240 article reviews
    gc tissue microarray chip - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation.
    Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti- Smad2 (no. 12570- 1- AP, Proteintech), anti- Smad3 (no. 66516- 1- Ig, Proteintech), anti- Smad4 (no. 10231- 1- AP, Proteintech), and anti- FOXN3 (no. ab129453, Abcam). ..

    Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation
    Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti-Smad2 (no. 12570-1-AP, Proteintech), anti-Smad3 (no. 66516-1-Ig, Proteintech), anti-Smad4 (no. 10231-1-AP, Proteintech), and anti-FOXN3 (no. ab129453, Abcam). ..

    Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation
    Article Snippet: A 10 % aliquot of each sheared chromatin sample was reserved as input control, reverse crosslinked, and purified using column-based cleanup (NucleoSpin, Takara). .. For immunoprecipitation samples, chromatin was incubated overnight at 4 °C with CUX1 antibody (11733-1-AP, Proteintech) in ChIP buffer (1 % Triton X-100, 0.1 % SDS, 150 mM NaCl, 2 mM EDTA, 20 mM Tris–HCl, pH 8.0) supplemented with protease inhibitors. .. Antibody-bound complexes were captured using Protein A/G magnetic beads (Thermo Fisher Scientific), washed sequentially with wash buffers at increasingly higher salt concentrations (0.1 % SDS, 0.1 % NaDOC, 1 % Triton-100, 1 mM EDTA, 20 mM Tris HCl, 150 mM NaCl or 500 mM NaCl, pH 8.0), 0.5 % NP-40 and 250 mM LiCl, followed by elution in ChIP elution buffer (1 % SDS, 0.1 M NaHCO 3 ).

    Article Title: Early growth response 1 promotes RNA polymerase I-directed transcription and cancer growth by activating RRN3 expression.
    Article Snippet: .. The anti-RPA43 antibody used for ChIP assays was purchased from the Proteintech Co. (Cat no. 22784-1-AP, Wuhan, China). .. Ten micrograms of antibodies against TFIIA/ (Customed from Frdbio), TFIIB (SC-225, Santa Cruz Biotech), the Pol II RPB1 subunit (ab76123, Abcam) and TBP (SC-421 Santa Cruz Biotech) were used for a ChIP assay for individual Pol II machinery factors.

    Real-time Polymerase Chain Reaction:

    Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation.
    Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti- Smad2 (no. 12570- 1- AP, Proteintech), anti- Smad3 (no. 66516- 1- Ig, Proteintech), anti- Smad4 (no. 10231- 1- AP, Proteintech), and anti- FOXN3 (no. ab129453, Abcam). ..

    Article Title: PARP1 stabilizes FOXN3 to suppress pulmonary fibrosis through p38-related feedback regulation
    Article Snippet: .. For ChIP quantitative PCR, the following antibodies were used: anti-Smad2 (no. 12570-1-AP, Proteintech), anti-Smad3 (no. 66516-1-Ig, Proteintech), anti-Smad4 (no. 10231-1-AP, Proteintech), and anti-FOXN3 (no. ab129453, Abcam). ..

    Immunoprecipitation:

    Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation
    Article Snippet: A 10 % aliquot of each sheared chromatin sample was reserved as input control, reverse crosslinked, and purified using column-based cleanup (NucleoSpin, Takara). .. For immunoprecipitation samples, chromatin was incubated overnight at 4 °C with CUX1 antibody (11733-1-AP, Proteintech) in ChIP buffer (1 % Triton X-100, 0.1 % SDS, 150 mM NaCl, 2 mM EDTA, 20 mM Tris–HCl, pH 8.0) supplemented with protease inhibitors. .. Antibody-bound complexes were captured using Protein A/G magnetic beads (Thermo Fisher Scientific), washed sequentially with wash buffers at increasingly higher salt concentrations (0.1 % SDS, 0.1 % NaDOC, 1 % Triton-100, 1 mM EDTA, 20 mM Tris HCl, 150 mM NaCl or 500 mM NaCl, pH 8.0), 0.5 % NP-40 and 250 mM LiCl, followed by elution in ChIP elution buffer (1 % SDS, 0.1 M NaHCO 3 ).

    Incubation:

    Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation
    Article Snippet: A 10 % aliquot of each sheared chromatin sample was reserved as input control, reverse crosslinked, and purified using column-based cleanup (NucleoSpin, Takara). .. For immunoprecipitation samples, chromatin was incubated overnight at 4 °C with CUX1 antibody (11733-1-AP, Proteintech) in ChIP buffer (1 % Triton X-100, 0.1 % SDS, 150 mM NaCl, 2 mM EDTA, 20 mM Tris–HCl, pH 8.0) supplemented with protease inhibitors. .. Antibody-bound complexes were captured using Protein A/G magnetic beads (Thermo Fisher Scientific), washed sequentially with wash buffers at increasingly higher salt concentrations (0.1 % SDS, 0.1 % NaDOC, 1 % Triton-100, 1 mM EDTA, 20 mM Tris HCl, 150 mM NaCl or 500 mM NaCl, pH 8.0), 0.5 % NP-40 and 250 mM LiCl, followed by elution in ChIP elution buffer (1 % SDS, 0.1 M NaHCO 3 ).

    Western Blot:

    Article Title: Reversible ubiquitination regulates HSF1 phase separation in response to proteotoxic stress.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    other:

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins
    Article Snippet: Anti-ACTB/β-actin antibody (66009-1-Ig), anti-GST antibody (HRP-66001), anti-STUB1 antibody (68407-1-Ig), anti-TOLLIP antibody (68170-1-Ig), horseradish peroxidase-labeled anti-rabbit and mouse antibodies, and IgG antibodies (SA00001-1, SA00001-2) were supplied by Proteintech.

    Article Title: CD81 drives immune evasion in radioresistant glioblastoma by blocking selective autophagic degradation of CD274/PD-L1.
    Article Snippet: Antibody Supplier Catalog # RRID Reactivity Application CD8A Servicebio GB114123-100 RRID: AB_3714641 H IHC (1:500) CD8A Servicebio GB15068-100 RRID: AB_3246431 M IF (1:200) CD81 Cell Signaling Technology (CST) 56,039 RRID: AB_2924772 H WB (1:1000) IHC (1:200) CD81 Abclonal A5270 RRID: AB_2766091 H/M PFA (1: 500) IF (1:200) CD81 Santa Cruz Biotechnology (SCB) sc-23962 RRID: AB_627192 H/M IP (1:100) CD81 CST 10,037 RRID: AB_2714207 M WB (1:1000) IHC (1:200) CD274/PD-L1 CST 13,684 RRID: AB_2864409 H WB (1:1000) IHC (1:200) CD274/PD-L1 SCB sc-293425 RRID: AB_3675453 H PFA (1:100) CD274/PD-L1 Proteintech 28,076–1-AP RRID: AB_2881052 H/M WB (1:1000) IHC (1:500) IF (1:100) CD274/PD-L1 CST E1L3N RRID: AB_3752922 H/M WB (1:1000) IHC (1:500) IF (1:200) Ub Proteintech 10,201–2-AP RRID: AB_671515 H/M IF (1:1000) USP14 Proteintech 14,517–1-AP RRID: AB_2257124 H/M WB (1:1000) IF (1:200) USP7 Proteintech 66,514–1-Ig RRID: AB_2881877 H/M WB (1:1000) STUB1 Proteintech 55,430–1-AP RRID: AB_10949225 H/M WB (1:1000) HUWE1 Proteintech 19,430–1-AP RRID: AB_2878579 H/M WB (1:1000) UBE2S Proteintech 14,115–1-AP RRID: AB_2256809 H/M WB (1:1000) SQSTM1/p62 Proteintech 18,420–1-AP RRID: AB_10694431 H/M WB (1:1000) IF (1:200) LC3 CST 3868 RRID: AB_2137707 H WB (1:1000) IF (1:500) AIF1/IBA-1 Servicebio GB12105 RRID: AB_2922434 M IF (1:500) MRC1/CD206 Servicebio GB113497 RRID: AB_3665021 M IF (1:500) ACTB/β-actin Proteintech 66,009–1-Ig RRID: AB_2687938 H/M WB (1:1000) Flag ABclonal AE004 RRID: AB_2771921 / WB (1:1000) IP (1:200) HA ABclonal AE105 RRID: AB_2943030 / WB (1:1000) MYC ABclonal AE070 RRID: AB_2770408 / WB (1:1000) IP (1:200) His ABclonal AE086 RRID: AB_3065205 / WB (1:1000) IP (1:200) HRP-anti-mouse CST 7076 RRID: AB_330924 / WB (1:5000) HRP-anti-rabbit CST 7074 RRID: AB_2099233 / WB (1:5000) Rabbit mAb IgG Isotype control CST 3900 RRID: AB_1550038 / PFA (1:500) Rabbit mAb IgG Isotype control CST 3423 RRID: AB_1550040 / IP (1:100) Mouse mAb IgG Isotype control CST 53,484 RRID: AB_2799435 / IP (1:100) PFA (1:500)

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins.
    Article Snippet: Anti-ACTB/β-actin antibody (66009-1-Ig), anti-GST antibody (HRP-66001), anti-STUB1 antibody (68407-1-Ig), anti-TOLLIP antibody (68170-1-Ig), horseradish peroxidase-labeled anti-rabbit and mouse antibodies, and IgG antibodies (SA00001-1, SA00001-2) were supplied by Proteintech.



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    Image Search Results


    PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue microarray. Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.

    Journal: Journal of Cancer

    Article Title: SP1 Mediated PIK3CB Upregulation Promotes Gastric Carcinogenesis

    doi: 10.7150/jca.83812

    Figure Lengend Snippet: PIK3CB and SP1 were overexpressed in GC samples. (A) The top 10 genes co-expressed with PIK3CB in TCGA-STAD datasets were shown. PCC: Pearson correlation coefficient. (B) The expression relevance between PIK3CB and SP1 was predicted by GEPIA online tools. (C) PIK3CB and SP1 were upregulated in GC tissue samples form TCGA database using GEPIA. (D) Kaplan-Meier curves for patients with different expression levels of PIK3CB or SP1 via Kaplan-Meier plotter database. (E) qRT-PCR analysis of PIK3CB and SP1 expression in 40 paired GC tissue and adjacent normal tissues. C/N score ≥1.5 was viewed as upregulated. (F) The assessment of correlation between PIK3CB and SP1 expression in 40 paired GC tissues using linear regression. (G) PIK3CB and SP1 expression level were evaluated by immunohistochemistry staining on a GC tissue microarray. Representative staining images and expression correlation of PI3KCB and SP1 in tumor tissue were shown. Scale bar: 50 μm. *P < 0.01.

    Article Snippet: The human GC tissue microarray containing 48 gastric cancer tissues and adjacent normal tissues were purchased from Zhuoli Biotech (Shanghai, China).

    Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining, Microarray