Review



fusion software  (Cytiva Europe)


Bioz Verified Symbol Cytiva Europe is a verified supplier
Bioz Manufacturer Symbol Cytiva Europe manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cytiva Europe fusion software
    Fusion Software, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 15195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+data+analysis+software+program/Fusion/pmc08886350-46-16-18
    Average 96 stars, based on 15195 article reviews
    fusion software - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Purification:

    Article Title: ETHYLENE RESPONSE FACTORS 4.1/4.2 with an EAR motif repress anthocyanin biosynthesis in red-skinned pears
    Article Snippet: .. Next, PyERF4.1-MBP, PyERF4.2-MBP, and PyERF3-HIS fusion proteins were expressed in DE3 Escherichia coli cells, and PyERF3 - HIS was purified using Ni Sepharose 6 Fast Flow (17-5318-06, GE Healthcare, Sweden). .. The collected elution extract was blotted with MBP or HIS antibodies (Beyotime, Shanghai, China).

    Article Title: A vaccine targeting resistant tumours by dual T cell plus NK cell attack
    Article Snippet: Eluted proteins were buffer exchanged into PBS and concentrated to 2 mg ml −1 using Amicon Ultra-15 centrifugal filters (EMD Millipore) with a 30-kDa molecular weight cut-off (MWCO). .. Concentrated proteins were purified further by HPLC size-exclusion chromatography using the following columns: Superose 6 Increase 10/300 GL (for human MICA α3–ferritin and MICB α3–ferritin), Superdex 200 (for ferritin) or tandem Superose 6 Increase 10/300 GL (for macaque MICA/B α3–ferritin) (GE Health-care) with PBS (fusion proteins) or HBS (ferritin) as running buffer at a flow rate of 0.8 ml min −1 . ..

    Article Title: Evolution of affinity between p53 transactivation domain and MDM2 across the animal kingdom demonstrates high plasticity of motif‐mediated interactions
    Article Snippet: Thereafter, cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs. .. The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size‐exclusion chromatography on a Hi load 16/60 Sephacryl S‐100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4. .. The fusion protein was then cleaved with PreScission (produced in house) protease overnight at 4°C followed by a second run on the size‐exclusion chromatography column to remove the NusA protein. in the case of pETM33 constructs, after removing cell debris the supernatant was loaded onto PierceTM Glutathione Agarose beads (Thermo Scientific), washed with wash buffer (50 mM Tris, 300 mM NaCl, pH 7.8) and eluted in GST elution buffer (50 mM Tris, 300 mM NaCl, 10 mM reduced glutathione, pH 7.8).

    High Performance Liquid Chromatography:

    Article Title: A vaccine targeting resistant tumours by dual T cell plus NK cell attack
    Article Snippet: Eluted proteins were buffer exchanged into PBS and concentrated to 2 mg ml −1 using Amicon Ultra-15 centrifugal filters (EMD Millipore) with a 30-kDa molecular weight cut-off (MWCO). .. Concentrated proteins were purified further by HPLC size-exclusion chromatography using the following columns: Superose 6 Increase 10/300 GL (for human MICA α3–ferritin and MICB α3–ferritin), Superdex 200 (for ferritin) or tandem Superose 6 Increase 10/300 GL (for macaque MICA/B α3–ferritin) (GE Health-care) with PBS (fusion proteins) or HBS (ferritin) as running buffer at a flow rate of 0.8 ml min −1 . ..

    Size-exclusion Chromatography:

    Article Title: A vaccine targeting resistant tumours by dual T cell plus NK cell attack
    Article Snippet: Eluted proteins were buffer exchanged into PBS and concentrated to 2 mg ml −1 using Amicon Ultra-15 centrifugal filters (EMD Millipore) with a 30-kDa molecular weight cut-off (MWCO). .. Concentrated proteins were purified further by HPLC size-exclusion chromatography using the following columns: Superose 6 Increase 10/300 GL (for human MICA α3–ferritin and MICB α3–ferritin), Superdex 200 (for ferritin) or tandem Superose 6 Increase 10/300 GL (for macaque MICA/B α3–ferritin) (GE Health-care) with PBS (fusion proteins) or HBS (ferritin) as running buffer at a flow rate of 0.8 ml min −1 . ..

    Article Title: Evolution of affinity between p53 transactivation domain and MDM2 across the animal kingdom demonstrates high plasticity of motif‐mediated interactions
    Article Snippet: Thereafter, cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs. .. The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size‐exclusion chromatography on a Hi load 16/60 Sephacryl S‐100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4. .. The fusion protein was then cleaved with PreScission (produced in house) protease overnight at 4°C followed by a second run on the size‐exclusion chromatography column to remove the NusA protein. in the case of pETM33 constructs, after removing cell debris the supernatant was loaded onto PierceTM Glutathione Agarose beads (Thermo Scientific), washed with wash buffer (50 mM Tris, 300 mM NaCl, pH 7.8) and eluted in GST elution buffer (50 mM Tris, 300 mM NaCl, 10 mM reduced glutathione, pH 7.8).

    Binding Assay:

    Article Title: Evolution of affinity between p53 transactivation domain and MDM2 across the animal kingdom demonstrates high plasticity of motif‐mediated interactions
    Article Snippet: Thereafter, cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs. .. The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size‐exclusion chromatography on a Hi load 16/60 Sephacryl S‐100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4. .. The fusion protein was then cleaved with PreScission (produced in house) protease overnight at 4°C followed by a second run on the size‐exclusion chromatography column to remove the NusA protein. in the case of pETM33 constructs, after removing cell debris the supernatant was loaded onto PierceTM Glutathione Agarose beads (Thermo Scientific), washed with wash buffer (50 mM Tris, 300 mM NaCl, pH 7.8) and eluted in GST elution buffer (50 mM Tris, 300 mM NaCl, 10 mM reduced glutathione, pH 7.8).

    Incubation:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells
    Article Snippet: cDNAs encoding CCP20, CCP22, or their chimeras were amplified by PCR and subcloned into pGEX4T-1 (GE Healthcare) at the EcoRI/SalI sites. .. GST fusion proteins were induced in Escherichia coli BL21 cells by incubation with 0.1 mM IPTG at 25°C for 2 h. The cells were lysed by sonication and the supernatants were passed over a glutathione-Sepharose 4B (GE Healthcare) column. ..

    Sonication:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells
    Article Snippet: cDNAs encoding CCP20, CCP22, or their chimeras were amplified by PCR and subcloned into pGEX4T-1 (GE Healthcare) at the EcoRI/SalI sites. .. GST fusion proteins were induced in Escherichia coli BL21 cells by incubation with 0.1 mM IPTG at 25°C for 2 h. The cells were lysed by sonication and the supernatants were passed over a glutathione-Sepharose 4B (GE Healthcare) column. ..



    Similar Products

    fusion data analysis software program  (Packard Instruments)
    90
    Packard Instruments fusion data analysis software program
    Fusion Data Analysis Software Program, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+data+analysis+software+program/fusion+data+analysis+software+program/pm24692238-83-5-10
    Average 90 stars, based on 1 article reviews
    fusion data analysis software program - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results