mtl1a cdnawas (OriGene)
OriGene is a verified supplier 
OriGene manufactures this product 
90
Structured Review
OriGene
mtl1a cdnawas
Mtl1a Cdnawas, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/function/pm23319737-62-1-5?v=OriGene
Average 90 stars, based on 1 article reviews
Mtl1a Cdnawas, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/function/pm23319737-62-1-5?v=OriGene
Average 90 stars, based on 1 article reviews
mtl1a cdnawas - by Bioz Stars,
2026-08
90/100 stars
Images
1) Product Images from "Cloning, expression, and functional characterization of TL1A-Ig."
Article Title: Cloning, expression, and functional characterization of TL1A-Ig.
Journal: Journal of immunology (Baltimore, Md. : 1950)
doi: 10.4049/jimmunol.1201908
Figure Legend Snippet: FIGURE 2. Characterization of purified TL1A-Ig by SDS-PAGE, Western blot, and gel filtration. (A) Purified TL1A-Ig was quantified using an mIgG-targeted ELISA. The nonreduced (22-ME) and reduced (+2-ME) TL1A-Ig samples were separated on SDS gel and stained with Coomassie blue or analyzed by immunoblotting using Abs to mTL1A or mIgG (B) SDS-PAGE separation of reduced TL1A-Ig after enzymatic deglycosyla- tion. Protein samples were treated with glycanases and separated on SDS- PAGE followed by staining with Coomassie blue stain. Denatured TL1A- Ig was loaded in each lane. Lane 1, Untreated control; lane 2, treated with N-Glycanase only; lane 3, treated with N-Glycanase and Sialidase A; and lane 4, treated with N-Glycanase, Sialidase A, and O-Glycanase. (C and D) Gel filtration analysis and model of expected hexameric oligomerization of TL1A-Ig. Purified TL1A-Ig (100 mg) was applied on a Superdex 200 column and subjected to size exclusion chromatography. The apparent molecular mass was calculated based on the elution volumes of the standards thyroglobulin (670 kDa), aldolase (158 kDa), and OVA (44 kDa).
Techniques Used: SDS Page, Western Blot, Enzyme-linked Immunosorbent Assay, SDS-Gel, Staining, Control, Size-exclusion Chromatography
![FIGURE 3. In vitro functional activity of TL1A-Ig. (A) P815 cells were stably transfected with expression vectors encoding mTNFRSF25. TNFRSF25- P815 cells were incubated with isotype mIgG1, TL1A-Ig (in culture supernatant or purified), or 4C12 and stained with fluorochrome-conjugated anti-IgG. TNFRSF25+ cells were then visualized by flow cytometry. (B) TNFRSF25-P815 cells were exposed to titrating concentrations of IgG1, TL1A-Ig, or 4C12. Cells were directly incubated with caspase substrate solution and free rhodamine 110 was determined fluorometrically. These data are representative of more than five experiments. (C) CD4+ T cells were purified from FIR mice and activated using plate-bound anti-CD3 for 4 d. Cells were harvested and incubated with isotype IgG or TL1A-Ig followed by staining with FITC anti-mouse IgG. mTL1A-Ig–bound TNFRSF25 was analyzed using flow cytometry by gating on CD4+FIR2 Tconvs and CD4+FIR+ Tregs. (D) CD4+CD252 Tconvs or (E) CD4+FIR+ Tregs were cultured in proliferation assays with indicated stimuli: anti- CD3 (2 mg/ml; 2C11), mIL-2 (10 U/ml), 4C12 (10 mg/ml), or purified TL1A-Ig (0.1 mg/ml) in triplicates for each condition. Data are representative of three independent experiments. (F) CD4+FIR+ Tregs were cultured in titrating concentration of cyclosporin A. Cultures in (D)–(F) were pulsed with [3H]thymidine for the last 6 h of 72-h incubation and incorporated isotope was measured by liquid scintillation counting. (G) For iTreg induction, FIR Tconvs were cultured with plate-bound anti-CD3, TGF-b, retinoic acid, mIL-2, and 4C12 or TL1A-Ig, and OT-II Tconvs were cultured as above plus 1:2 APCs and OVA. Cultures were analyzed for Foxp3+ cells in the CD4 gate. One representative analysis of three independent experiments is shown. ***p , 0.001 versus appropriate control. Significance was determined by one-way ANOVA with Tukey posttest (D, E). Error bars indicate mean 6 SEM. NV, Not visible.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_9737/pm23319737/pm23319737__page6_image1.jpg "... TL1A-Ig followed by staining with FITC anti-mouse IgG. mTL1A-Ig–bound TNFRSF25 was analyzed using flow cytometry by gating ...")
Figure Legend Snippet: FIGURE 3. In vitro functional activity of TL1A-Ig. (A) P815 cells were stably transfected with expression vectors encoding mTNFRSF25. TNFRSF25- P815 cells were incubated with isotype mIgG1, TL1A-Ig (in culture supernatant or purified), or 4C12 and stained with fluorochrome-conjugated anti-IgG. TNFRSF25+ cells were then visualized by flow cytometry. (B) TNFRSF25-P815 cells were exposed to titrating concentrations of IgG1, TL1A-Ig, or 4C12. Cells were directly incubated with caspase substrate solution and free rhodamine 110 was determined fluorometrically. These data are representative of more than five experiments. (C) CD4+ T cells were purified from FIR mice and activated using plate-bound anti-CD3 for 4 d. Cells were harvested and incubated with isotype IgG or TL1A-Ig followed by staining with FITC anti-mouse IgG. mTL1A-Ig–bound TNFRSF25 was analyzed using flow cytometry by gating on CD4+FIR2 Tconvs and CD4+FIR+ Tregs. (D) CD4+CD252 Tconvs or (E) CD4+FIR+ Tregs were cultured in proliferation assays with indicated stimuli: anti- CD3 (2 mg/ml; 2C11), mIL-2 (10 U/ml), 4C12 (10 mg/ml), or purified TL1A-Ig (0.1 mg/ml) in triplicates for each condition. Data are representative of three independent experiments. (F) CD4+FIR+ Tregs were cultured in titrating concentration of cyclosporin A. Cultures in (D)–(F) were pulsed with [3H]thymidine for the last 6 h of 72-h incubation and incorporated isotope was measured by liquid scintillation counting. (G) For iTreg induction, FIR Tconvs were cultured with plate-bound anti-CD3, TGF-b, retinoic acid, mIL-2, and 4C12 or TL1A-Ig, and OT-II Tconvs were cultured as above plus 1:2 APCs and OVA. Cultures were analyzed for Foxp3+ cells in the CD4 gate. One representative analysis of three independent experiments is shown. ***p , 0.001 versus appropriate control. Significance was determined by one-way ANOVA with Tukey posttest (D, E). Error bars indicate mean 6 SEM. NV, Not visible.
Techniques Used: In Vitro, Functional Assay, Activity Assay, Stable Transfection, Transfection, Expressing, Incubation, Staining, Cytometry, Cell Culture, Concentration Assay, Control
Figure Legend Snippet: FIGURE 4. mTL1A-Ig stimulates rapid proliferation of CD4+Foxp3+ Tregs in vivo. (A) The time-related serum concentrations of 4C12 and TL1A-Ig in C57BL/6 mice (n = 3–5) was determined after a single i.p injection of 100 mg 4C12 or TL1A-Ig. Protein concentration was measured in serum samples collected at indicated time points by a sandwich ELISA specific for Armenian hamster IgG or TL1A. Concentration at each time point was calculated as a percentage of the average initial serum concentration (stable agonist concentration after equilibration between blood and tissues). Unique symbol represents each 4C12 treated (solid) mouse or TL1A-Ig (hollow) mouse. (B) The kinetics and dose-dependent expansion of Tregs in peripheral blood were determined after i.p injection of 100 mg IgG (n = 3) or TL1A-Ig (n = 4) in FIR mice for 3 consecutive days as indicated by the arrows. Mice were bled daily and the percentage of peripheral Tregs relative to total CD4+ cells was determined by flow cytometry. (C) Treg expansion was monitored in the peripheral blood while administering daily i.p injections of 100 mg IgG (n = 2) or TL1A-Ig (n = 4) starting on day 0 and ending on day 20. (D) Ten million CD4+ cells were highly purified by FACS sorting from FIR mice and adoptively transferred into CD742/2 or CD42/2 mice. After 3 d (day 0), recipient mice were treated with 100 mg TL1A-Ig or IgG followed by two consecutive doses on days 1 and 2. The percentage of Foxp3+ cells was analyzed in the spleen and pooled lymph nodes on day 6. Data are representative of two independent experiments, with two or more mice per group. Statistical analysis was performed by an unpaired two-tailed Student t test (B, C). All data are means 6 SEM (B–D). *p , 0.05, **p , 0.01, ***p , 0.001 versus control.
Techniques Used: In Vivo, Injection, Protein Concentration, Sandwich ELISA, Concentration Assay, Cytometry, Two Tailed Test, Control
