frap data analysis (Nikon)
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frap data analysis
Frap Data Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frap+data+analysis/NIS-Elements/pm39824183-1012-0-5
Average 99 stars, based on 39666 article reviews
Frap Data Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frap+data+analysis/NIS-Elements/pm39824183-1012-0-5
Average 99 stars, based on 39666 article reviews
frap data analysis - by Bioz Stars,
2026-09
99/100 stars
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Software:Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. Obtained images were processed and analyzed using Article Title: Pathogenicity, immunogenicity, and cross-protective efficacy of representative Eimeria brunetti strains in Chinese yellow-feathered broilers: an in vivo evaluation Article Snippet: Sporulated oocysts of each geographical strain, obtained via single-oocyst inoculation, were examined under an inverted fluorescence microscope (Nikon Eclipse Ti2, Japan) at 400 × magnification. .. For each strain, images of 100 oocysts were captured, and their length and width were measured using the Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: Subsequently, they were sequentially incubated with AF647-conjugated anti-Flag antibody (1:500, Sigma‒Aldrich, Germany), FITC-labeled phalloidin (1:1000, Yeasen Biotechnology, China), and DAPI nuclear stain (1:1000, Sigma‒Aldrich, Germany). .. After washing with PBS, multichannel fluorescence imaging was performed using a Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: .. Multichannel fluorescence images were captured using a Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. Three-dimensional structures were reconstructed using Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. Images were processed using Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis Article Snippet: .. Total-internal-reflectance fluorescence (TIRF) microscopy was used to perform imaging in the highly inclined and laminated optical sheet (HILO) mode using ND acquisition in Nikon Fluorescence:Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: Subsequently, they were sequentially incubated with AF647-conjugated anti-Flag antibody (1:500, Sigma‒Aldrich, Germany), FITC-labeled phalloidin (1:1000, Yeasen Biotechnology, China), and DAPI nuclear stain (1:1000, Sigma‒Aldrich, Germany). .. After washing with PBS, multichannel fluorescence imaging was performed using a Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: .. Multichannel fluorescence images were captured using a Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis Article Snippet: .. Total-internal-reflectance fluorescence (TIRF) microscopy was used to perform imaging in the highly inclined and laminated optical sheet (HILO) mode using ND acquisition in Nikon Imaging:Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: Subsequently, they were sequentially incubated with AF647-conjugated anti-Flag antibody (1:500, Sigma‒Aldrich, Germany), FITC-labeled phalloidin (1:1000, Yeasen Biotechnology, China), and DAPI nuclear stain (1:1000, Sigma‒Aldrich, Germany). .. After washing with PBS, multichannel fluorescence imaging was performed using a Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. Three-dimensional structures were reconstructed using Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis Article Snippet: .. Total-internal-reflectance fluorescence (TIRF) microscopy was used to perform imaging in the highly inclined and laminated optical sheet (HILO) mode using ND acquisition in Nikon Microscopy:Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: Subsequently, they were sequentially incubated with AF647-conjugated anti-Flag antibody (1:500, Sigma‒Aldrich, Germany), FITC-labeled phalloidin (1:1000, Yeasen Biotechnology, China), and DAPI nuclear stain (1:1000, Sigma‒Aldrich, Germany). .. After washing with PBS, multichannel fluorescence imaging was performed using a Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: .. Multichannel fluorescence images were captured using a Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. Three-dimensional structures were reconstructed using Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis Article Snippet: .. Total-internal-reflectance fluorescence (TIRF) microscopy was used to perform imaging in the highly inclined and laminated optical sheet (HILO) mode using ND acquisition in Nikon |
Table S3 ). (G) Scheme showing 97 Hz and 2 Hz SPT workflow. Three-state model fits to 97 Hz SPT data using Spot-On was used to derive fractions and diffusion coefficients of fast diffusing ( F fast , D fast ), slow diffusing ( F slow , D slow ), and bound PARP ( F bound , D bound ) molecules. Further, 2 Hz SPT data were fit using a two-phase exponential model to derive fractions and duration of transient (Fraction transient, τ transient ) and stable (Fraction stable, τ stable ) PARP binding events. (H and I) Pie chart illustrations summarizing the derivation of overall fractions of Halo-PARP1 (in H) and Halo-PARP2 (in I) engaging in transient and stable binding, slow diffusion, and fast diffusion from 97 to 2 Hz SPT experiments. The bound, slow, and fast-diffusing fractions (in i) were determined using Spot-On’s three-state model fitting to 97 Hz SPT data. The bound fraction (in ii) in Halo-PARP1 and Halo-PARP2 cells was analyzed by 2 Hz SPT and fit to a two-phase exponential model. Data acquired for H2B-Halo were used for photobleaching correction, and a correction factor (see ) was applied to obtain the true fraction of transiently and stably binding Halo-PARP molecules (in iii). These data were compiled together to obtain the overall fractions of endogenous Halo-PARP1 and Halo-PARP2 molecules (in iv). (J) Normalized and photobleaching corrected recovery curves from 
