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formulation buffer  (Cytiva Europe)


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    Structured Review

    Cytiva Europe formulation buffer
    Formulation Buffer, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/formulation+buffer/Formulation+buffer/pmc06290686-73-8-15
    Average 93 stars, based on 20 article reviews
    formulation buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Formulation:

    Article Title: Preclinical evaluation reveals comparable toxicology and pharmacology of the erythropoietin biosimilar GBpoietin® and Eprex®
    Article Snippet: Erythropoietin (EPO) is an essential growth factor for erythropoiesis.. We report the results of the preclinical safety evaluation of GBpoietin®, a recombinant human EPO (rhEPO), through a comparative acute toxicity study with the reference product, Eprex®.. The products were administered subcutaneously into Wistar rats for both the single-dose and repeated-dose toxicity studies.

    Article Title: SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape
    Article Snippet: Mammalian cell lines Cell lines were received from ATCC (Vero E6, Vero, BHK-21, CHO-K1, HEK293T/17), Takara (Lenti-X 293T) and Thermo Fisher Scientific (ExpiCHO-S, Expi293F and Freestyle 293-F). .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Article Title: Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry
    Article Snippet: MAbs affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Article Title: Broad sarbecovirus neutralization by a human monoclonal antibody
    Article Snippet: Affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). .. One day post-transfection, cells were infected with VSV-luc (VSV-G) (Kerafast, EH1020-PM) for 1 h, rinsed three times with PBS, then incubated for an additional 24 h in complete media at 37°C.

    Article Title: A human antibody that broadly neutralizes betacoronaviruses protects against SARS-CoV-2 by blocking the fusion machinery
    Article Snippet: MAbs affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Article Title: Reliability of task-specific neuronal activation assessed with functional PET, ASL and BOLD imaging
    Article Snippet: .. The radioactive glucose analogue [ F]FDG was freshly synthesized every morning using FASTlab FDG cassettes with phosphate buffer formulation and a FASTlab platform (GE Healthcare). .. The substance was administered via a cubital vein as bolus for 1 minute followed by constant infusion for 51 minutes with an infusion pump (Syramed μSP6000, Arcomed, Switzerland, dosage: 5.1 MBq/kg, bolus speed: 816 ml/h, infusion speed: 42.8 ml/h, bolus-infusion ratio of activity: 20:80%), which was placed in an MR-shield.

    Injection:

    Article Title: Preclinical evaluation reveals comparable toxicology and pharmacology of the erythropoietin biosimilar GBpoietin® and Eprex®
    Article Snippet: Erythropoietin (EPO) is an essential growth factor for erythropoiesis.. We report the results of the preclinical safety evaluation of GBpoietin®, a recombinant human EPO (rhEPO), through a comparative acute toxicity study with the reference product, Eprex®.. The products were administered subcutaneously into Wistar rats for both the single-dose and repeated-dose toxicity studies.

    Buffer Exchange:

    Article Title: SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape
    Article Snippet: Mammalian cell lines Cell lines were received from ATCC (Vero E6, Vero, BHK-21, CHO-K1, HEK293T/17), Takara (Lenti-X 293T) and Thermo Fisher Scientific (ExpiCHO-S, Expi293F and Freestyle 293-F). .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Article Title: Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry
    Article Snippet: MAbs affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Article Title: Broad sarbecovirus neutralization by a human monoclonal antibody
    Article Snippet: Affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). .. One day post-transfection, cells were infected with VSV-luc (VSV-G) (Kerafast, EH1020-PM) for 1 h, rinsed three times with PBS, then incubated for an additional 24 h in complete media at 37°C.

    Article Title: Mutant fragments of OspA and methods and uses relating thereto
    Article Snippet: The resulting flow-through was loaded on a hydroxyapatite column (Bio-Rad) and the lipidated proteins were eluted from the column by a linear salt gradient. .. The eluate was subjected to further purification over a DEAE-Sepharose column (GE Healthcare) in non-binding mode followed by gel filtration column (Superdex 200, GE Healthcare) for buffer exchange. ..

    Article Title: A human antibody that broadly neutralizes betacoronaviruses protects against SARS-CoV-2 by blocking the fusion machinery
    Article Snippet: MAbs affinity purification was performed on ÄKTA Xpress FPLC (Cytiva) operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments, using PBS as mobile phase. .. Buffer exchange to the appropriate formulation buffer was performed with a HiTrap Fast desalting column (Cytiva). ..

    Purification:

    Article Title: Mutant fragments of OspA and methods and uses relating thereto
    Article Snippet: The resulting flow-through was loaded on a hydroxyapatite column (Bio-Rad) and the lipidated proteins were eluted from the column by a linear salt gradient. .. The eluate was subjected to further purification over a DEAE-Sepharose column (GE Healthcare) in non-binding mode followed by gel filtration column (Superdex 200, GE Healthcare) for buffer exchange. ..

    Filtration:

    Article Title: Mutant fragments of OspA and methods and uses relating thereto
    Article Snippet: The resulting flow-through was loaded on a hydroxyapatite column (Bio-Rad) and the lipidated proteins were eluted from the column by a linear salt gradient. .. The eluate was subjected to further purification over a DEAE-Sepharose column (GE Healthcare) in non-binding mode followed by gel filtration column (Superdex 200, GE Healthcare) for buffer exchange. ..

    Synthesized:

    Article Title: Reliability of task-specific neuronal activation assessed with functional PET, ASL and BOLD imaging
    Article Snippet: .. The radioactive glucose analogue [ F]FDG was freshly synthesized every morning using FASTlab FDG cassettes with phosphate buffer formulation and a FASTlab platform (GE Healthcare). .. The substance was administered via a cubital vein as bolus for 1 minute followed by constant infusion for 51 minutes with an infusion pump (Syramed μSP6000, Arcomed, Switzerland, dosage: 5.1 MBq/kg, bolus speed: 816 ml/h, infusion speed: 42.8 ml/h, bolus-infusion ratio of activity: 20:80%), which was placed in an MR-shield.



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