formaldehyde (fa) solution (Fisher Scientific)
90
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Fisher Scientific
formaldehyde (fa) solution
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Formaldehyde (Fa) Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde+fa+solution/formaldehyde++fa++solution/pmc08674535-57-0-4
Average 90 stars, based on 1 article reviews
Figure S1 . " width="250" height="auto" />Formaldehyde (Fa) Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde+fa+solution/formaldehyde++fa++solution/pmc08674535-57-0-4
Average 90 stars, based on 1 article reviews
formaldehyde (fa) solution - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability"
Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability
Journal: Cell Reports
doi: 10.1016/j.celrep.2021.110080
Figure S1 . " title="DPCs are modified by SUMO and ubiquitin (A) Formaldehyde (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: DPCs are modified by SUMO and ubiquitin (A) Formaldehyde (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also
Techniques Used: Modification, Ubiquitin Proteomics, Isolation, Staining, Western Blot, Control
Figure Legend Snippet:
Techniques Used: Virus, Subcloning, Recombinant, Staining, Picogreen Assay, Proliferation Assay, Flow Cytometry, DNA Extraction, Sequencing, Luciferase, Software, Transfection, Modification, Magnetic Beads, Membrane
Related Articles
Modification:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Ubiquitin Proteomics:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Isolation:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Staining:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Western Blot:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Control:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Virus:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Subcloning:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Recombinant:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Picogreen Assay:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Proliferation Assay:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Flow Cytometry:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: DNA Extraction:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Sequencing:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Luciferase:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Software:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Transfection:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Magnetic Beads:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: Membrane:Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Article Snippet: |
