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Structured Review

Virtek Vision International chipreader microarray scanner
(A) Cluster images showing genes differentially expressed in this series of isolates. Red represents genes that are up-regulated, and green represents genes that are down-regulated. Genes shown in both cluster images appear in the same order. Genes are grouped as being coordinately differentially expressed with CaMDR1 (I), CDR genes (II), neither CaMDR1 nor CDR genes (III), or both CaMDR1 and CDR genes (IV). (B) Confirmation of differential expression by RT-PCR of select genes found to be differentially expressed by cDNA <t>microarray</t> analysis. RT-PCR of C. albicans 18S rRNA was performed as a control.
Chipreader Microarray Scanner, supplied by Virtek Vision International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Genome-Wide Expression Profile Analysis Reveals Coordinately Regulated Genes Associated with Stepwise Acquisition of Azole Resistance in Candida albicans Clinical Isolates"

Article Title: Genome-Wide Expression Profile Analysis Reveals Coordinately Regulated Genes Associated with Stepwise Acquisition of Azole Resistance in Candida albicans Clinical Isolates

Journal:

doi: 10.1128/AAC.47.4.1220-1227.2003

(A) Cluster images showing genes differentially expressed in this series of isolates. Red represents genes that are up-regulated, and green represents genes that are down-regulated. Genes shown in both cluster images appear in the same order. Genes are grouped as being coordinately differentially expressed with CaMDR1 (I), CDR genes (II), neither CaMDR1 nor CDR genes (III), or both CaMDR1 and CDR genes (IV). (B) Confirmation of differential expression by RT-PCR of select genes found to be differentially expressed by cDNA microarray analysis. RT-PCR of C. albicans 18S rRNA was performed as a control.
Figure Legend Snippet: (A) Cluster images showing genes differentially expressed in this series of isolates. Red represents genes that are up-regulated, and green represents genes that are down-regulated. Genes shown in both cluster images appear in the same order. Genes are grouped as being coordinately differentially expressed with CaMDR1 (I), CDR genes (II), neither CaMDR1 nor CDR genes (III), or both CaMDR1 and CDR genes (IV). (B) Confirmation of differential expression by RT-PCR of select genes found to be differentially expressed by cDNA microarray analysis. RT-PCR of C. albicans 18S rRNA was performed as a control.

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray

Related Articles

Control:

Article Title: Gradient resolved information platform
Article Snippet: .. The slide is scanned along with various control treatments with a microarray scanner (ChipReaderTM, Virtek Vision, Waterloo, ON, Canada) at the Cy5 wavelength. ..

Microarray:

Article Title: Gradient resolved information platform
Article Snippet: .. The slide is scanned along with various control treatments with a microarray scanner (ChipReaderTM, Virtek Vision, Waterloo, ON, Canada) at the Cy5 wavelength. ..

Article Title: Gene expression profiling of the response of Streptococcus pneumoniae to penicillin.
Article Snippet: .. Slides were scanned using a ChipReader microarray scanner (Virtek Vision Intl, Waterloo, Ontario, Canada). .. GenePix 1.0 software (Molecular Devices, Sunnyvale, CA, USA) was used for image analysis.

Article Title: Genome-wide expression profiling of the response to ciclopirox olamine in Candida albicans.
Article Snippet: .. Slides were scanned using a ChipReader microarray scanner (Virtek Vision Intl). .. GenePix 1.0 software (Axon Instruments, Inc.) was used for image analysis and data visualization.

Article Title: Gallic Acid Inhibited Matrix Invasion and AP-1/ETS-1-Mediated MMP-1 Transcription in Human Nasopharyngeal Carcinoma Cells
Article Snippet: Fluorescent labeling of target mRNA was prepared by indirect 3DNA Submicro Expression Array Detection kit (Genisphere, Hatfield, PA, USA). .. RNA was reverse transcribed to target cDNA using oligo-d(T) primer tagged with either Cys-3 or Cys-5-specific 3 DNA-capture sequences and hybridized to microarray for 16 h. Next step, the in situ labeling with Cys-3DNA or Cys-5-3DNA based on the sequence-complementation to the capture sequences was carried out for 2 h. After washing, slides were scanned with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, ON, Canada). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Sunnyvale, CA, USA) and carried out within-slide normalization with programs written by MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, USA).

Labeling:

Article Title: Interleukin-1 receptor antagonist (IL-1RA) prevents apoptosis in ex vivo expansion of human limbal epithelial cells cultivated on human amniotic membrane.
Article Snippet: .. We used 10 g of RNA for labeling and hybridization using 3DNA Submicro EX Expression Array kit (Genisphere, Hatfield, PA, http:// www.genisphere.com), and scanned slides with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, Ontario, Canada, http://www.virtekvision.com). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Union City, CA, http://www.moleculardevices.com) and carried out withinslide normalization with programs wrote with MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, http://www. mathworks.com).

Article Title: Gallic Acid Inhibited Matrix Invasion and AP-1/ETS-1-Mediated MMP-1 Transcription in Human Nasopharyngeal Carcinoma Cells
Article Snippet: Fluorescent labeling of target mRNA was prepared by indirect 3DNA Submicro Expression Array Detection kit (Genisphere, Hatfield, PA, USA). .. RNA was reverse transcribed to target cDNA using oligo-d(T) primer tagged with either Cys-3 or Cys-5-specific 3 DNA-capture sequences and hybridized to microarray for 16 h. Next step, the in situ labeling with Cys-3DNA or Cys-5-3DNA based on the sequence-complementation to the capture sequences was carried out for 2 h. After washing, slides were scanned with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, ON, Canada). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Sunnyvale, CA, USA) and carried out within-slide normalization with programs written by MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, USA).

Hybridization:

Article Title: Interleukin-1 receptor antagonist (IL-1RA) prevents apoptosis in ex vivo expansion of human limbal epithelial cells cultivated on human amniotic membrane.
Article Snippet: .. We used 10 g of RNA for labeling and hybridization using 3DNA Submicro EX Expression Array kit (Genisphere, Hatfield, PA, http:// www.genisphere.com), and scanned slides with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, Ontario, Canada, http://www.virtekvision.com). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Union City, CA, http://www.moleculardevices.com) and carried out withinslide normalization with programs wrote with MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, http://www. mathworks.com).

Article Title: S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer
Article Snippet: The GMRCL Human 7K set, version 1 chips (InCyte Genomics, Palo Alto, CA, USA) containing 7,334 sequence-verified human genes were used in the present study. .. Following hybridization and washing, the slides were scanned using a confocal scanner ChipReader (Virtek Vision International, Waterloo, ON, Canada). .. An indirect labeling of cDNA targets using the 3DNA Sunmicro EX Expression Array Detection kit (Genisphere, Hatfield, PA, USA) was used.

Expressing:

Article Title: Interleukin-1 receptor antagonist (IL-1RA) prevents apoptosis in ex vivo expansion of human limbal epithelial cells cultivated on human amniotic membrane.
Article Snippet: .. We used 10 g of RNA for labeling and hybridization using 3DNA Submicro EX Expression Array kit (Genisphere, Hatfield, PA, http:// www.genisphere.com), and scanned slides with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, Ontario, Canada, http://www.virtekvision.com). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Union City, CA, http://www.moleculardevices.com) and carried out withinslide normalization with programs wrote with MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, http://www. mathworks.com).

Generated:

Article Title: Novel light-regulated genes in Trichoderma atroviride: a dissection by cDNA microarrays.
Article Snippet: .. The slides were scanned using a confocal laser scanner (Chipreader, Virtek Vision) at a resolution of 10 mm and the data files generated were collected and analysed with the ArrayPro Analyser software (Media Cybernetics). ..

Software:

Article Title: Novel light-regulated genes in Trichoderma atroviride: a dissection by cDNA microarrays.
Article Snippet: .. The slides were scanned using a confocal laser scanner (Chipreader, Virtek Vision) at a resolution of 10 mm and the data files generated were collected and analysed with the ArrayPro Analyser software (Media Cybernetics). ..

Reverse Transcription:

Article Title: Gallic Acid Inhibited Matrix Invasion and AP-1/ETS-1-Mediated MMP-1 Transcription in Human Nasopharyngeal Carcinoma Cells
Article Snippet: Fluorescent labeling of target mRNA was prepared by indirect 3DNA Submicro Expression Array Detection kit (Genisphere, Hatfield, PA, USA). .. RNA was reverse transcribed to target cDNA using oligo-d(T) primer tagged with either Cys-3 or Cys-5-specific 3 DNA-capture sequences and hybridized to microarray for 16 h. Next step, the in situ labeling with Cys-3DNA or Cys-5-3DNA based on the sequence-complementation to the capture sequences was carried out for 2 h. After washing, slides were scanned with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, ON, Canada). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Sunnyvale, CA, USA) and carried out within-slide normalization with programs written by MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, USA).

In Situ:

Article Title: Gallic Acid Inhibited Matrix Invasion and AP-1/ETS-1-Mediated MMP-1 Transcription in Human Nasopharyngeal Carcinoma Cells
Article Snippet: Fluorescent labeling of target mRNA was prepared by indirect 3DNA Submicro Expression Array Detection kit (Genisphere, Hatfield, PA, USA). .. RNA was reverse transcribed to target cDNA using oligo-d(T) primer tagged with either Cys-3 or Cys-5-specific 3 DNA-capture sequences and hybridized to microarray for 16 h. Next step, the in situ labeling with Cys-3DNA or Cys-5-3DNA based on the sequence-complementation to the capture sequences was carried out for 2 h. After washing, slides were scanned with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, ON, Canada). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Sunnyvale, CA, USA) and carried out within-slide normalization with programs written by MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, USA).

Sequencing:

Article Title: Gallic Acid Inhibited Matrix Invasion and AP-1/ETS-1-Mediated MMP-1 Transcription in Human Nasopharyngeal Carcinoma Cells
Article Snippet: Fluorescent labeling of target mRNA was prepared by indirect 3DNA Submicro Expression Array Detection kit (Genisphere, Hatfield, PA, USA). .. RNA was reverse transcribed to target cDNA using oligo-d(T) primer tagged with either Cys-3 or Cys-5-specific 3 DNA-capture sequences and hybridized to microarray for 16 h. Next step, the in situ labeling with Cys-3DNA or Cys-5-3DNA based on the sequence-complementation to the capture sequences was carried out for 2 h. After washing, slides were scanned with a confocal scanner ChipReader (Virtek Vision International Inc., Waterloo, ON, Canada). .. We acquired the spot and background intensities with GenePix Pro 4.1 software (Axon Instruments/Molecular Devices Corp., Sunnyvale, CA, USA) and carried out within-slide normalization with programs written by MATLAB 6.0 software (The MathWorks, Inc., Natick, MA, USA).



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