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memglow488  (Cytoskeleton Inc)


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    Cytoskeleton Inc memglow488
    Memglow488, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+probe/MemGlow+488+Fluorogenic+Membrane+Probe/pm42049809-214-8-9
    Average 96 stars, based on 61 article reviews
    memglow488 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry
    Article Snippet: .. For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT). ..

    Article Title: Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.
    Article Snippet: .. The membrane staining was done using 40-50 nM MemGlow488 (Cytoskeleton, Inc., Cat# MG01), which was kept in the medium during imaging, for the U2OS-Nup107, U2OS β4Gal-T1, RPE-1 and U2OS wildtype cell lines. ..

    Cell Culture:

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry
    Article Snippet: .. For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT). ..

    Incubation:

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry
    Article Snippet: .. For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT). ..

    Clinical Proteomics:

    Article Title: A clickable CoQ imaging probe reveals that cellular uptake and lysosomal trafficking depend on CD36 and NPC1
    Article Snippet: .. To visualize the plasma membrane, the fluorescent probe MemGlow 488 (Cytoskeleton) was used at a final concentration of 1 μM after the SPAAC reaction. l ) First row, from left to right, representative confocal images of cells costained with CoQ Azide, Hoechst, and LysoTracker Green, along with m ) the corresponding colocalization analysis of CoQ Azide signal with lysosomes. n ) Costaining of CoQ Azide, Hoechst, and MitoTracker Green in the second row, and o ) respective colocalization of CoQ Azide signal with MitoTracker Green. p ) Third row, representative confocal images of cell costained with CoQ Azide, Hoechst and MEMGlow and q ) respective colocalization analysis of CoQ Azide signal with plasma membrane. ..

    Membrane:

    Article Title: A clickable CoQ imaging probe reveals that cellular uptake and lysosomal trafficking depend on CD36 and NPC1
    Article Snippet: .. To visualize the plasma membrane, the fluorescent probe MemGlow 488 (Cytoskeleton) was used at a final concentration of 1 μM after the SPAAC reaction. l ) First row, from left to right, representative confocal images of cells costained with CoQ Azide, Hoechst, and LysoTracker Green, along with m ) the corresponding colocalization analysis of CoQ Azide signal with lysosomes. n ) Costaining of CoQ Azide, Hoechst, and MitoTracker Green in the second row, and o ) respective colocalization of CoQ Azide signal with MitoTracker Green. p ) Third row, representative confocal images of cell costained with CoQ Azide, Hoechst and MEMGlow and q ) respective colocalization analysis of CoQ Azide signal with plasma membrane. ..

    Article Title: Curvature-Encoded Photochemistry for Programmable Isolation and Fractionation of Extracellular and Plasma Membrane Vesicles
    Article Snippet: Monomeric avidin agarose beads (High Capacity) were purchased from Thermo Scientific. .. The fluorogenic membrane dye MemGlow-488 was obtained from Cytoskeleton, Inc. Recombinant VAMP2 (v-SNARE) and Syntaxin-1A/SNAP-25 (t-SNARE) proteins were expressed and purified from E. coli as described previously. .. [ , ] Non-photoreactive chemical probes were purchased from BroadPharm and Nanocs.

    Article Title: Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.
    Article Snippet: .. The membrane staining was done using 40-50 nM MemGlow488 (Cytoskeleton, Inc., Cat# MG01), which was kept in the medium during imaging, for the U2OS-Nup107, U2OS β4Gal-T1, RPE-1 and U2OS wildtype cell lines. ..

    Concentration Assay:

    Article Title: A clickable CoQ imaging probe reveals that cellular uptake and lysosomal trafficking depend on CD36 and NPC1
    Article Snippet: .. To visualize the plasma membrane, the fluorescent probe MemGlow 488 (Cytoskeleton) was used at a final concentration of 1 μM after the SPAAC reaction. l ) First row, from left to right, representative confocal images of cells costained with CoQ Azide, Hoechst, and LysoTracker Green, along with m ) the corresponding colocalization analysis of CoQ Azide signal with lysosomes. n ) Costaining of CoQ Azide, Hoechst, and MitoTracker Green in the second row, and o ) respective colocalization of CoQ Azide signal with MitoTracker Green. p ) Third row, representative confocal images of cell costained with CoQ Azide, Hoechst and MEMGlow and q ) respective colocalization analysis of CoQ Azide signal with plasma membrane. ..

    Recombinant:

    Article Title: Curvature-Encoded Photochemistry for Programmable Isolation and Fractionation of Extracellular and Plasma Membrane Vesicles
    Article Snippet: Monomeric avidin agarose beads (High Capacity) were purchased from Thermo Scientific. .. The fluorogenic membrane dye MemGlow-488 was obtained from Cytoskeleton, Inc. Recombinant VAMP2 (v-SNARE) and Syntaxin-1A/SNAP-25 (t-SNARE) proteins were expressed and purified from E. coli as described previously. .. [ , ] Non-photoreactive chemical probes were purchased from BroadPharm and Nanocs.

    Purification:

    Article Title: Curvature-Encoded Photochemistry for Programmable Isolation and Fractionation of Extracellular and Plasma Membrane Vesicles
    Article Snippet: Monomeric avidin agarose beads (High Capacity) were purchased from Thermo Scientific. .. The fluorogenic membrane dye MemGlow-488 was obtained from Cytoskeleton, Inc. Recombinant VAMP2 (v-SNARE) and Syntaxin-1A/SNAP-25 (t-SNARE) proteins were expressed and purified from E. coli as described previously. .. [ , ] Non-photoreactive chemical probes were purchased from BroadPharm and Nanocs.

    Imaging:

    Article Title: Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.
    Article Snippet: .. The membrane staining was done using 40-50 nM MemGlow488 (Cytoskeleton, Inc., Cat# MG01), which was kept in the medium during imaging, for the U2OS-Nup107, U2OS β4Gal-T1, RPE-1 and U2OS wildtype cell lines. ..



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    (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye <t>MemGlow-488.</t> (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.
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    Image Search Results


    (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Journal: medRxiv

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry

    doi: 10.64898/2026.04.28.26351941

    Figure Lengend Snippet: (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Article Snippet: For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT).

    Techniques: Staining, Virus, Infection