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Becton Dickinson cellfix (1%) solution
Cellfix (1%) Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixative+solution/cell+fix+solution/pmc09104366-239-6-7
Average 90 stars, based on 1 article reviews
cellfix (1%) solution - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: The course of allergen-induced leukocyte infiltration in human and experimental asthma.
Article Snippet: a ll e rg ic in fl a m m a ti o n Background: Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.. Objective: To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.. Methods: Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge.

Article Title: Variations in the Gene Expression Profile in Atherosclerotic Patients with Non-Fatal ACS: A Preliminary Study
Article Snippet: Subsequently, 500 μL of CellFix (1%) solution (Becton Dickinson, San Diego, CA, USA) was added to fix the samples, and each sample was centrifuged (5000× g , for 10 min.).

Article Title: Lentivirus-induced ‘Smart' dendritic cells: Pharmacodynamics and GMP-compliant production for immunotherapy against TRP2-positive melanoma
Article Snippet: Cells were washed and resuspended in cell-fix solution (Becton Dickinson).

Article Title: CD95/Fas expression on peripheral blood T lymphocytes in patients with multiple sclerosis: effect of high-dose methylprednisolone therapy.
Article Snippet: Recent data indicate that the apoptotic process, mediated by the CD95/Fas cell surface receptor, is impaired in activated lymphocytes of patients with relapsing-remitting multiple sclerosis.. Using flow cytometric-immunophenotyping, we analyzed the expression of CD95/Fas on peripheral blood CD4+ and CD8+ T lymphocytes (PBL) in 10 MS patients in relapse, and the effect of pulse corticosteroid therapy on the apoptosis of autoreactive lymphocytes.. The proportions of CD8+ and CD8+CD95+ T lymphocytes were significantly higher in MS patients in relapse before than after pulse corticosteroid therapy.

Article Title: Individual Contributions of Mutant Protease and Reverse Transcriptase to Viral Infectivity, Replication, and Protein Maturation of Antiretroviral Drug-Resistant Human Immunodeficiency Virus Type 1
Article Snippet: Cells were trypsinized 24 h postinfection, harvested in 700 ml of phosphate-buffered saline–5% FCS–2 mM EDTA, and resuspended in 250 ml of cell-fixing solution (Becton Dickinson, Erembodegem, Belgium).

Article Title: Autologous Human Dendritic Cells from XDR-TB Patients Polarize a Th1 Response Which Is Bactericidal to Mycobacterium tuberculosis .
Article Snippet: Thereafter, they were washed and fixed with 1% Cell Fix solution (Becton-Dickenson, USA).

Article Title: EXOGENOUS IL-12 AND ITS EFFECT ON TH1/TH2 CELL ACTIVITY AFTER CARDIAC SURGERY
Article Snippet: There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery.. We hypothesized that a postoperative decrease in interferon (IFN) +Yproducing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression.. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days.

FACS:

Article Title: The course of allergen-induced leukocyte infiltration in human and experimental asthma.
Article Snippet: a ll e rg ic in fl a m m a ti o n Background: Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.. Objective: To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.. Methods: Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge.

Article Title: Variations in the Gene Expression Profile in Atherosclerotic Patients with Non-Fatal ACS: A Preliminary Study
Article Snippet: Subsequently, 500 μL of CellFix (1%) solution (Becton Dickinson, San Diego, CA, USA) was added to fix the samples, and each sample was centrifuged (5000× g , for 10 min.).

Article Title: Lentivirus-induced ‘Smart' dendritic cells: Pharmacodynamics and GMP-compliant production for immunotherapy against TRP2-positive melanoma
Article Snippet: Cells were washed and resuspended in cell-fix solution (Becton Dickinson).

Article Title: CD95/Fas expression on peripheral blood T lymphocytes in patients with multiple sclerosis: effect of high-dose methylprednisolone therapy.
Article Snippet: Recent data indicate that the apoptotic process, mediated by the CD95/Fas cell surface receptor, is impaired in activated lymphocytes of patients with relapsing-remitting multiple sclerosis.. Using flow cytometric-immunophenotyping, we analyzed the expression of CD95/Fas on peripheral blood CD4+ and CD8+ T lymphocytes (PBL) in 10 MS patients in relapse, and the effect of pulse corticosteroid therapy on the apoptosis of autoreactive lymphocytes.. The proportions of CD8+ and CD8+CD95+ T lymphocytes were significantly higher in MS patients in relapse before than after pulse corticosteroid therapy.

Article Title: Individual Contributions of Mutant Protease and Reverse Transcriptase to Viral Infectivity, Replication, and Protein Maturation of Antiretroviral Drug-Resistant Human Immunodeficiency Virus Type 1
Article Snippet: Cells were trypsinized 24 h postinfection, harvested in 700 ml of phosphate-buffered saline–5% FCS–2 mM EDTA, and resuspended in 250 ml of cell-fixing solution (Becton Dickinson, Erembodegem, Belgium).

Article Title: Autologous Human Dendritic Cells from XDR-TB Patients Polarize a Th1 Response Which Is Bactericidal to Mycobacterium tuberculosis .
Article Snippet: Thereafter, they were washed and fixed with 1% Cell Fix solution (Becton-Dickenson, USA).

Article Title: EXOGENOUS IL-12 AND ITS EFFECT ON TH1/TH2 CELL ACTIVITY AFTER CARDIAC SURGERY
Article Snippet: There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery.. We hypothesized that a postoperative decrease in interferon (IFN) +Yproducing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression.. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days.

Flow Cytometry:

Article Title: The course of allergen-induced leukocyte infiltration in human and experimental asthma.
Article Snippet: a ll e rg ic in fl a m m a ti o n Background: Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.. Objective: To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.. Methods: Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge.

Article Title: Variations in the Gene Expression Profile in Atherosclerotic Patients with Non-Fatal ACS: A Preliminary Study
Article Snippet: Subsequently, 500 μL of CellFix (1%) solution (Becton Dickinson, San Diego, CA, USA) was added to fix the samples, and each sample was centrifuged (5000× g , for 10 min.).

Article Title: Lentivirus-induced ‘Smart' dendritic cells: Pharmacodynamics and GMP-compliant production for immunotherapy against TRP2-positive melanoma
Article Snippet: Cells were washed and resuspended in cell-fix solution (Becton Dickinson).

Article Title: CD95/Fas expression on peripheral blood T lymphocytes in patients with multiple sclerosis: effect of high-dose methylprednisolone therapy.
Article Snippet: Recent data indicate that the apoptotic process, mediated by the CD95/Fas cell surface receptor, is impaired in activated lymphocytes of patients with relapsing-remitting multiple sclerosis.. Using flow cytometric-immunophenotyping, we analyzed the expression of CD95/Fas on peripheral blood CD4+ and CD8+ T lymphocytes (PBL) in 10 MS patients in relapse, and the effect of pulse corticosteroid therapy on the apoptosis of autoreactive lymphocytes.. The proportions of CD8+ and CD8+CD95+ T lymphocytes were significantly higher in MS patients in relapse before than after pulse corticosteroid therapy.

Article Title: Individual Contributions of Mutant Protease and Reverse Transcriptase to Viral Infectivity, Replication, and Protein Maturation of Antiretroviral Drug-Resistant Human Immunodeficiency Virus Type 1
Article Snippet: Cells were trypsinized 24 h postinfection, harvested in 700 ml of phosphate-buffered saline–5% FCS–2 mM EDTA, and resuspended in 250 ml of cell-fixing solution (Becton Dickinson, Erembodegem, Belgium).

Article Title: Autologous Human Dendritic Cells from XDR-TB Patients Polarize a Th1 Response Which Is Bactericidal to Mycobacterium tuberculosis .
Article Snippet: Thereafter, they were washed and fixed with 1% Cell Fix solution (Becton-Dickenson, USA).

Article Title: EXOGENOUS IL-12 AND ITS EFFECT ON TH1/TH2 CELL ACTIVITY AFTER CARDIAC SURGERY
Article Snippet: There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery.. We hypothesized that a postoperative decrease in interferon (IFN) +Yproducing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression.. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days.

Saline:

Article Title: The course of allergen-induced leukocyte infiltration in human and experimental asthma.
Article Snippet: a ll e rg ic in fl a m m a ti o n Background: Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.. Objective: To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.. Methods: Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge.

Article Title: Variations in the Gene Expression Profile in Atherosclerotic Patients with Non-Fatal ACS: A Preliminary Study
Article Snippet: Subsequently, 500 μL of CellFix (1%) solution (Becton Dickinson, San Diego, CA, USA) was added to fix the samples, and each sample was centrifuged (5000× g , for 10 min.).

Article Title: Lentivirus-induced ‘Smart' dendritic cells: Pharmacodynamics and GMP-compliant production for immunotherapy against TRP2-positive melanoma
Article Snippet: Cells were washed and resuspended in cell-fix solution (Becton Dickinson).

Article Title: CD95/Fas expression on peripheral blood T lymphocytes in patients with multiple sclerosis: effect of high-dose methylprednisolone therapy.
Article Snippet: Recent data indicate that the apoptotic process, mediated by the CD95/Fas cell surface receptor, is impaired in activated lymphocytes of patients with relapsing-remitting multiple sclerosis.. Using flow cytometric-immunophenotyping, we analyzed the expression of CD95/Fas on peripheral blood CD4+ and CD8+ T lymphocytes (PBL) in 10 MS patients in relapse, and the effect of pulse corticosteroid therapy on the apoptosis of autoreactive lymphocytes.. The proportions of CD8+ and CD8+CD95+ T lymphocytes were significantly higher in MS patients in relapse before than after pulse corticosteroid therapy.

Article Title: Individual Contributions of Mutant Protease and Reverse Transcriptase to Viral Infectivity, Replication, and Protein Maturation of Antiretroviral Drug-Resistant Human Immunodeficiency Virus Type 1
Article Snippet: Cells were trypsinized 24 h postinfection, harvested in 700 ml of phosphate-buffered saline–5% FCS–2 mM EDTA, and resuspended in 250 ml of cell-fixing solution (Becton Dickinson, Erembodegem, Belgium).

Article Title: Autologous Human Dendritic Cells from XDR-TB Patients Polarize a Th1 Response Which Is Bactericidal to Mycobacterium tuberculosis .
Article Snippet: Thereafter, they were washed and fixed with 1% Cell Fix solution (Becton-Dickenson, USA).

Article Title: EXOGENOUS IL-12 AND ITS EFFECT ON TH1/TH2 CELL ACTIVITY AFTER CARDIAC SURGERY
Article Snippet: There is controversy about the origin of the alterations in T helper 1 (TH1)/TH2 cell activity after major surgical procedures such as on-pump cardiac surgery.. We hypothesized that a postoperative decrease in interferon (IFN) +Yproducing TH1 lymphocyte activity may be the sole cause of this TH1/TH2 shift and that the addition of recombinant IL-12 can reverse TH1 suppression.. Peripheral blood mononuclear cell fractions from 20 low-risk elective cardiac surgery patients were analyzed preoperatively (d0) and on the first (d1), third (d3), and sixth (d6) postoperative days.



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