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Servicebio Inc paraformaldehyde fix solution g1101
Paraformaldehyde Fix Solution G1101, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixative+solution/g1101/pm42249111-272-2-9
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paraformaldehyde fix solution g1101 - by Bioz Stars, 2026-10
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Bicinchoninic Acid Protein Assay:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Enzyme-linked Immunosorbent Assay:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Multiple Displacement Amplification:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Lysis:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Blocking Assay:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

SDS Page:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

CCK-8 Assay:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

ROS Assay:

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNF-alpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Article Title: Ellagic Acid Prevents Obesity in High-Fat Diet-Fed Rats by Ameliorating Oxidative Stress via Modulation of the PPARG/STAT3/p-AKT1 Axis.
Article Snippet: .. Ellagic acid (purity ≥ 98%, R016669) (Roan Chemical Technology, Shanghai, China); the BCA protein assay kit (PC0020), the Tumor Necrosis Factor-alpha (TNF-α) ELISA kit (SEKR-0009), and the Interleukin-6 (IL-6) ELISA kit (SEKR-0005) (Solarbio, Beijing, China); https://doi.org/10.3390/foods15050890 total superoxide dismutase (SOD, A001-1-2) and malondialdehyde (MDA, A003-1-2) assay kits (Jiancheng, Nanjing, China); RIPA lysis buffer (ab288006) (Abcam, Cambridge, UK); 5% BSA blocking buffer and PBS buffer (Solarbio, Beijing, China); 1% osmium tetroxide (Ted Pella Inc., Redding, CA, USA), uranyl acetate (SPI, West Chester, PA, USA), and lead citrate (Sigma-Aldrich, St. Louis, MO, USA); tissue fixative (G1101) (Servicebio, Wuhan, China); antibodies against PPARG (1:2000, K113849P), STAT3 (1:1000, K000233M), AKT1 (1:1000, K000186M), p-AKT1 (1:1000, K012659RR), β-actin (1:2000, K200058M), and GAPDH (1:5000, K200057M), the SDS-PAGE gel preparation kit (Solarbio, Beijing, China); Anti-Human TNFalpha (Polyclonal Rabbit, PHC3013, PeproTech, Cranbury, NJ, USA); the Cell Counting Kit-8 (Solarbio, Beijing, China); the ROS assay kit (Yeasen Biotechnology, Shanghai, China); DMEM high-glucose medium, fetal bovine serum (FBS), and trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) were all acquired. .. A low-speed benchtop centrifuge (TDL-5000bR, Linjia, Shanghai, China) and an automated biochemical analyzer (Lx-20, Beckman Coulter, Brea, CA, USA) were utilized.

Staining:

Article Title: Reduced YTHDF2 inhibits PD-L1 expression by stabilizing m 6 A-containing SPOP mRNA in colorectal cancer.
Article Snippet: .. Formalin-fixed (G1101, Servicebio, Hubei, P. R. China) and paraffin-embedded samples were sectioned at 5 μm and stained with hematoxylin and eosin (G1005, Servicebio, Hubei, P. R. China). .. Immunohistochemistry (IHC) was performed on samples using YTHDF2 antibody (ab246514, Abcam, Cambridge, MA, UK), PD-L1 antibody (13684, CST, BOS, USA), or CD8 (ab235951, Abcam, Cambridge, MA, UK).

Virus:

Article Title: Blood-brain barrier-penetrative lipid nanoparticles enable systemic delivery of TRIM11 mRNA to disaggregate Tau in Alzheimer's disease models.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Tau (P-Ser396) HUABIO Cat#ET1611-68;RRID:AB_3070047 Tau HUABIO Cat#ET1612-44;RRID:AB_3070118 DYKDDDDK Tag Flag Cell Signaling Technology Cat#8146;RRID:AB_10950495 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermofisher Cat#MN1020;RRID:AB_223647 NF-L Monoclonal Antibody HUABIO Cat#HA721538;RRID:AB_3072654 GFAP Polyclonal antibody Proteintech Cat#16825-1-AP;RRID:AB_2109646 Iba-1 Polyclonal antibody Proteintech Cat#10904-1-AP;RRID:AB_2224377 β3-Tubulin Monoclonal Antibody HUABIO Cat#ET1602-4;RRID:AB_2938592 GAPDH Monoclonal Antibody HUABIO Cat#HA721136;RRID:AB_3072260 TRIM11 Proteintech Cat#10851-1-AP;RRID:AB_2209210 Bacterial and virus strains pCDNA3.0 Addgene Addgene plasmid#136622 Chemicals, peptides and recombinant proteins Okadaic acid Yuanye Cat#S30686;CAS:78111-17-8 Tau-441/2N4R Pre-formed Fibr-ils Protein (PFFs) Acrobiosystems Cat#TAU-H5115 Distearoyl Phosphatidylcholine (DSPC) Macklin Cat#D742457;CAS:816-94-4 N,N′-dicyclohexylcarbodiimide (DCC) Macklin Cat#N806920;CAS:538-75-0 Cholesterol Macklin Cat#C769358;CAS:57-88-5 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000) Macklin Cat#D890330;CAS:160743-62-4 Heptadecan-9-yl 8-((2-hydroxyethyl)(6-oxo6-(undecyloxy)hexyl)amino)octanoate (SM-102) Macklin Cat#H960267;CAS:2089251-47-6 2,2′-Azobis(2-methylpropionitrile) (AIBN) Macklin Cat#A800353;CAS:78-67-1 Phosphoric Acid 2-(Methacryloyloxy)ethyl 2-(Trimethylammonio)ethyl Ester (MPC) Aladdin Cat#M158414;CAS:67881-98-5 4-Cyano-4-(phenylcarbonothioylthio)pentanoic Acid (CPADN) Macklin Cat#C833222;CAS:201611-92-9 N-hydroxysuccinimide (NHS) Macklin Cat#130672;CAS:6066-82-6 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) Macklin CatD130471;CAS:1069-79-0 Paraformaldehyde (PFA) Servicebio Cat#G1101 RIPA buffer Solarbio Cat#R0020 Lipofectamine 2000 Invitrogene Cat#11668-019 Trypsin-EDTA (0.25%) Gibco Cat#A11105-01 4′,6-diamidino-2-phenylindole(DAPI, Thermo-FisherScientific) Solarbio Cat#C0065 Cy5-conjugated goat anti-rabbit IgG Servicebio Cat#GB27303 Cy5-conjugated goat anti-mouse IgG Servicebio Cat#GB27301 FITC-conjugated goat anti-mouse IgG Servicebio Cat#GB22301 FITC-conjugated goat anti-rabbit IgG Servicebio Cat#GB22303 PMSF (100 mM) Servicebio Cat#G2008 Triton X-100 BioBasic Cat#BT0198 Skim milk Servicebio Cat#GC310001 FBS Gibco Cat#A5256701 High Glucose DMEM Pricella Cat#PM150210 RIPA buffer Solarbio Cat#R0020 (Continued on next page) Cell Reports Medicine 7, 102685, March 17, 2026 e1 ..

Plasmid Preparation:

Article Title: Blood-brain barrier-penetrative lipid nanoparticles enable systemic delivery of TRIM11 mRNA to disaggregate Tau in Alzheimer's disease models.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Tau (P-Ser396) HUABIO Cat#ET1611-68;RRID:AB_3070047 Tau HUABIO Cat#ET1612-44;RRID:AB_3070118 DYKDDDDK Tag Flag Cell Signaling Technology Cat#8146;RRID:AB_10950495 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermofisher Cat#MN1020;RRID:AB_223647 NF-L Monoclonal Antibody HUABIO Cat#HA721538;RRID:AB_3072654 GFAP Polyclonal antibody Proteintech Cat#16825-1-AP;RRID:AB_2109646 Iba-1 Polyclonal antibody Proteintech Cat#10904-1-AP;RRID:AB_2224377 β3-Tubulin Monoclonal Antibody HUABIO Cat#ET1602-4;RRID:AB_2938592 GAPDH Monoclonal Antibody HUABIO Cat#HA721136;RRID:AB_3072260 TRIM11 Proteintech Cat#10851-1-AP;RRID:AB_2209210 Bacterial and virus strains pCDNA3.0 Addgene Addgene plasmid#136622 Chemicals, peptides and recombinant proteins Okadaic acid Yuanye Cat#S30686;CAS:78111-17-8 Tau-441/2N4R Pre-formed Fibr-ils Protein (PFFs) Acrobiosystems Cat#TAU-H5115 Distearoyl Phosphatidylcholine (DSPC) Macklin Cat#D742457;CAS:816-94-4 N,N′-dicyclohexylcarbodiimide (DCC) Macklin Cat#N806920;CAS:538-75-0 Cholesterol Macklin Cat#C769358;CAS:57-88-5 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000) Macklin Cat#D890330;CAS:160743-62-4 Heptadecan-9-yl 8-((2-hydroxyethyl)(6-oxo6-(undecyloxy)hexyl)amino)octanoate (SM-102) Macklin Cat#H960267;CAS:2089251-47-6 2,2′-Azobis(2-methylpropionitrile) (AIBN) Macklin Cat#A800353;CAS:78-67-1 Phosphoric Acid 2-(Methacryloyloxy)ethyl 2-(Trimethylammonio)ethyl Ester (MPC) Aladdin Cat#M158414;CAS:67881-98-5 4-Cyano-4-(phenylcarbonothioylthio)pentanoic Acid (CPADN) Macklin Cat#C833222;CAS:201611-92-9 N-hydroxysuccinimide (NHS) Macklin Cat#130672;CAS:6066-82-6 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) Macklin CatD130471;CAS:1069-79-0 Paraformaldehyde (PFA) Servicebio Cat#G1101 RIPA buffer Solarbio Cat#R0020 Lipofectamine 2000 Invitrogene Cat#11668-019 Trypsin-EDTA (0.25%) Gibco Cat#A11105-01 4′,6-diamidino-2-phenylindole(DAPI, Thermo-FisherScientific) Solarbio Cat#C0065 Cy5-conjugated goat anti-rabbit IgG Servicebio Cat#GB27303 Cy5-conjugated goat anti-mouse IgG Servicebio Cat#GB27301 FITC-conjugated goat anti-mouse IgG Servicebio Cat#GB22301 FITC-conjugated goat anti-rabbit IgG Servicebio Cat#GB22303 PMSF (100 mM) Servicebio Cat#G2008 Triton X-100 BioBasic Cat#BT0198 Skim milk Servicebio Cat#GC310001 FBS Gibco Cat#A5256701 High Glucose DMEM Pricella Cat#PM150210 RIPA buffer Solarbio Cat#R0020 (Continued on next page) Cell Reports Medicine 7, 102685, March 17, 2026 e1 ..

Recombinant:

Article Title: Blood-brain barrier-penetrative lipid nanoparticles enable systemic delivery of TRIM11 mRNA to disaggregate Tau in Alzheimer's disease models.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Tau (P-Ser396) HUABIO Cat#ET1611-68;RRID:AB_3070047 Tau HUABIO Cat#ET1612-44;RRID:AB_3070118 DYKDDDDK Tag Flag Cell Signaling Technology Cat#8146;RRID:AB_10950495 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermofisher Cat#MN1020;RRID:AB_223647 NF-L Monoclonal Antibody HUABIO Cat#HA721538;RRID:AB_3072654 GFAP Polyclonal antibody Proteintech Cat#16825-1-AP;RRID:AB_2109646 Iba-1 Polyclonal antibody Proteintech Cat#10904-1-AP;RRID:AB_2224377 β3-Tubulin Monoclonal Antibody HUABIO Cat#ET1602-4;RRID:AB_2938592 GAPDH Monoclonal Antibody HUABIO Cat#HA721136;RRID:AB_3072260 TRIM11 Proteintech Cat#10851-1-AP;RRID:AB_2209210 Bacterial and virus strains pCDNA3.0 Addgene Addgene plasmid#136622 Chemicals, peptides and recombinant proteins Okadaic acid Yuanye Cat#S30686;CAS:78111-17-8 Tau-441/2N4R Pre-formed Fibr-ils Protein (PFFs) Acrobiosystems Cat#TAU-H5115 Distearoyl Phosphatidylcholine (DSPC) Macklin Cat#D742457;CAS:816-94-4 N,N′-dicyclohexylcarbodiimide (DCC) Macklin Cat#N806920;CAS:538-75-0 Cholesterol Macklin Cat#C769358;CAS:57-88-5 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000) Macklin Cat#D890330;CAS:160743-62-4 Heptadecan-9-yl 8-((2-hydroxyethyl)(6-oxo6-(undecyloxy)hexyl)amino)octanoate (SM-102) Macklin Cat#H960267;CAS:2089251-47-6 2,2′-Azobis(2-methylpropionitrile) (AIBN) Macklin Cat#A800353;CAS:78-67-1 Phosphoric Acid 2-(Methacryloyloxy)ethyl 2-(Trimethylammonio)ethyl Ester (MPC) Aladdin Cat#M158414;CAS:67881-98-5 4-Cyano-4-(phenylcarbonothioylthio)pentanoic Acid (CPADN) Macklin Cat#C833222;CAS:201611-92-9 N-hydroxysuccinimide (NHS) Macklin Cat#130672;CAS:6066-82-6 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) Macklin CatD130471;CAS:1069-79-0 Paraformaldehyde (PFA) Servicebio Cat#G1101 RIPA buffer Solarbio Cat#R0020 Lipofectamine 2000 Invitrogene Cat#11668-019 Trypsin-EDTA (0.25%) Gibco Cat#A11105-01 4′,6-diamidino-2-phenylindole(DAPI, Thermo-FisherScientific) Solarbio Cat#C0065 Cy5-conjugated goat anti-rabbit IgG Servicebio Cat#GB27303 Cy5-conjugated goat anti-mouse IgG Servicebio Cat#GB27301 FITC-conjugated goat anti-mouse IgG Servicebio Cat#GB22301 FITC-conjugated goat anti-rabbit IgG Servicebio Cat#GB22303 PMSF (100 mM) Servicebio Cat#G2008 Triton X-100 BioBasic Cat#BT0198 Skim milk Servicebio Cat#GC310001 FBS Gibco Cat#A5256701 High Glucose DMEM Pricella Cat#PM150210 RIPA buffer Solarbio Cat#R0020 (Continued on next page) Cell Reports Medicine 7, 102685, March 17, 2026 e1 ..

Droplet Countercurrent Chromatography:

Article Title: Blood-brain barrier-penetrative lipid nanoparticles enable systemic delivery of TRIM11 mRNA to disaggregate Tau in Alzheimer's disease models.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Tau (P-Ser396) HUABIO Cat#ET1611-68;RRID:AB_3070047 Tau HUABIO Cat#ET1612-44;RRID:AB_3070118 DYKDDDDK Tag Flag Cell Signaling Technology Cat#8146;RRID:AB_10950495 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermofisher Cat#MN1020;RRID:AB_223647 NF-L Monoclonal Antibody HUABIO Cat#HA721538;RRID:AB_3072654 GFAP Polyclonal antibody Proteintech Cat#16825-1-AP;RRID:AB_2109646 Iba-1 Polyclonal antibody Proteintech Cat#10904-1-AP;RRID:AB_2224377 β3-Tubulin Monoclonal Antibody HUABIO Cat#ET1602-4;RRID:AB_2938592 GAPDH Monoclonal Antibody HUABIO Cat#HA721136;RRID:AB_3072260 TRIM11 Proteintech Cat#10851-1-AP;RRID:AB_2209210 Bacterial and virus strains pCDNA3.0 Addgene Addgene plasmid#136622 Chemicals, peptides and recombinant proteins Okadaic acid Yuanye Cat#S30686;CAS:78111-17-8 Tau-441/2N4R Pre-formed Fibr-ils Protein (PFFs) Acrobiosystems Cat#TAU-H5115 Distearoyl Phosphatidylcholine (DSPC) Macklin Cat#D742457;CAS:816-94-4 N,N′-dicyclohexylcarbodiimide (DCC) Macklin Cat#N806920;CAS:538-75-0 Cholesterol Macklin Cat#C769358;CAS:57-88-5 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000) Macklin Cat#D890330;CAS:160743-62-4 Heptadecan-9-yl 8-((2-hydroxyethyl)(6-oxo6-(undecyloxy)hexyl)amino)octanoate (SM-102) Macklin Cat#H960267;CAS:2089251-47-6 2,2′-Azobis(2-methylpropionitrile) (AIBN) Macklin Cat#A800353;CAS:78-67-1 Phosphoric Acid 2-(Methacryloyloxy)ethyl 2-(Trimethylammonio)ethyl Ester (MPC) Aladdin Cat#M158414;CAS:67881-98-5 4-Cyano-4-(phenylcarbonothioylthio)pentanoic Acid (CPADN) Macklin Cat#C833222;CAS:201611-92-9 N-hydroxysuccinimide (NHS) Macklin Cat#130672;CAS:6066-82-6 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) Macklin CatD130471;CAS:1069-79-0 Paraformaldehyde (PFA) Servicebio Cat#G1101 RIPA buffer Solarbio Cat#R0020 Lipofectamine 2000 Invitrogene Cat#11668-019 Trypsin-EDTA (0.25%) Gibco Cat#A11105-01 4′,6-diamidino-2-phenylindole(DAPI, Thermo-FisherScientific) Solarbio Cat#C0065 Cy5-conjugated goat anti-rabbit IgG Servicebio Cat#GB27303 Cy5-conjugated goat anti-mouse IgG Servicebio Cat#GB27301 FITC-conjugated goat anti-mouse IgG Servicebio Cat#GB22301 FITC-conjugated goat anti-rabbit IgG Servicebio Cat#GB22303 PMSF (100 mM) Servicebio Cat#G2008 Triton X-100 BioBasic Cat#BT0198 Skim milk Servicebio Cat#GC310001 FBS Gibco Cat#A5256701 High Glucose DMEM Pricella Cat#PM150210 RIPA buffer Solarbio Cat#R0020 (Continued on next page) Cell Reports Medicine 7, 102685, March 17, 2026 e1 ..

Saline:

Article Title: Restoring cardiolipin homeostasis mitigates cerebral ischemia-reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis.
Article Snippet: Following the manufacturer’s instructions and based on preliminary experiments, Neuro-2a cells were transfected with the LVs at a titer of 1 × 108 TU/ml, supplemented with the viral infection enhancement solution HiTransG P (Genechem, Shanghai, China) for 16 h. After transfection, the cells were changed to complete culture medium and cultured for an additional 72 h. Stably transduced cells were selected using 5 lM puromycin and validated by western blotting or RT-qPCR. .. Euthanized mice were intracardially perfused with normal saline and 4% paraformaldehyde (PFA, Servicebio, G1101, Wuhan, China). ..

Article Title: Parental gut microbiota dysbiosis is associated with cross-generationally shared DNA methylation patterns and autism-like behaviors in offspring.
Article Snippet: .. For mice designated for histological analysis, animals were deeply anesthetized with 3% isoflurane AR TI CL E IN P RE SS and then transcranial perfused with phosphate-buffered saline (PBS) followed by 4% (w/v) paraformaldehyde (PFA; Servicebio, G1101, China) for brain and colon collection. ..



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