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tonbo fix permeabilization solution  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences tonbo fix permeabilization solution
    Tonbo Fix Permeabilization Solution, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixation+buffer+solution/Fixation+Buffer/pmc12355481-57-22-22
    Average 95 stars, based on 1 article reviews
    tonbo fix permeabilization solution - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Mechanisms of superior respiratory IgA responses against SARS-CoV-2 after mucosal vaccination
    Article Snippet: .. To stain Blimp-1, after surface and viability staining, cells were fixed with fixation buffer (Cytek Biosciences) and permeabilized with Perm buffer (Cytek Biosciences), followed by staining at RT for 1 h. After staining, cells were washed twice with Perm/Wash buffer (BioLegend) to reduce the background. .. Cell population data were acquired on Attune NxT (Thermo Fisher Scientific) and analyzed using FlowJo Software (10.8.1, Tree Star Inc.).

    Article Title: Subunit Vaccination Using Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) Platform Elicits Enhanced Humoral and Cellular Immune Responses
    Article Snippet: Cells were washed with Flow Staining Buffer (Cytek) and stained with the viability dye (Ghost Dye Red 780 Fixable Viability Dye, Cell Signaling Technology), CD19 (6D5, BioLegend), CD44 (IM-7, Tonbo), CD127 (A7R34, Tonbo), KLRG1 (2F1/KLRG1, BioLegend) for 20 min at RT. .. Samples were washed, fixed for 30 min in Fixation Buffer (Tonbo), washed with Flow Staining Buffer, and permeabilized for five min with 1X Flow Cytometry Perm Buffer (Tonbo). .. After permeabilization, the samples were stained intracellularly with Granzyme B (QA16A02, BioLegend) for one h RT and resuspended in Flow Stain Buffer (Tonbo).

    Article Title: Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) platform provides unique antigen delivery system through controlled release to improve immune response to vaccination
    Article Snippet: Cells were then washed with complete media and stained with the viability dye (Ghost Dye Red 780 Fixable Viability Dye, Cell Signaling Technology), CD19 (6D5, BioLegend), CD44 (IM-7, Tonbo) in Flow Staining Buffer (Cytek) for 20 minutes RT. .. After washing with Flow Staining Buffer, the samples were fixed for 30 minutes in Fixation Buffer (Cytek), washed again, and resuspended in Flow Staining Buffer. .. Samples were acquired on Cytek Northern Lights 3-laser (VBR) spectral flow cytometer and analyzed using FlowJo 10.10.0 software (BD Biosciences), Excel (Microsoft), and GraphPad Prism (version 10.4.2).

    Flow Cytometry:

    Article Title: Subunit Vaccination Using Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) Platform Elicits Enhanced Humoral and Cellular Immune Responses
    Article Snippet: Cells were washed with Flow Staining Buffer (Cytek) and stained with the viability dye (Ghost Dye Red 780 Fixable Viability Dye, Cell Signaling Technology), CD19 (6D5, BioLegend), CD44 (IM-7, Tonbo), CD127 (A7R34, Tonbo), KLRG1 (2F1/KLRG1, BioLegend) for 20 min at RT. .. Samples were washed, fixed for 30 min in Fixation Buffer (Tonbo), washed with Flow Staining Buffer, and permeabilized for five min with 1X Flow Cytometry Perm Buffer (Tonbo). .. After permeabilization, the samples were stained intracellularly with Granzyme B (QA16A02, BioLegend) for one h RT and resuspended in Flow Stain Buffer (Tonbo).

    FACS:

    Article Title: Distinct commensal bacteria in human nasopharyngeal lymphoid tissue associated with localized immunological memory
    Article Snippet: .. The surface-stained cells were fixed for 30 min at room temperature in fixation buffer, washed with permeabilization buffer (Tonbo (Cytek Biosciences), Fremont, CA, USA. catalog no. TNB-0607-KIT), and washed again with FACS buffer. .. Flow cytometry data were collected using a five-laser Cytek Aurora flow cytometer (Cytek Biosciences) and analyzed using FlowJo V 10.7 software ( ).



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