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fcr blocking reagent, human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fcr blocking reagent, human
    Fcr Blocking Reagent, Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fcr+block/FcR+Blocking+Reagent%2C+human/custom%40130-059-901%4042409814
    Average 97 stars, based on 3333 article reviews
    fcr blocking reagent, human - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Endothelial senescence impairs efferocytosis and alters macrophage responses: insights into APP-CD74 signaling
    Article Snippet: .. Prior to staining, cells were incubated for 10 min at 4 °C with human FcR Blocking Reagent (#130-059-901, Miltenyi Biotec). .. Subsequently, the cells were aliquoted and stained for 30 min on ice and protected from light with the following antibody panels: (i), PE-Cy7-conjugated anti-CD169 (clone 7-239, #346014, BioLegend, 1:25), and APC-conjugated anti-HLA-DR (clone G46-6, #559866, BD Pharmingen, 1:10); (ii) AF488-conjugated anti-CD206 (clone 15-2, #321114, BioLegend, 1:20); (iii) FITC-conjugated anti-CD11b (clone ICRF44, #301330, BioLegend, 1:25), PE-conjugated anti-CD163 (clone GHI/61, #556018, BD Pharmingen, 1:10), PE-Cy7-conjugated anti-PD-L1 (clone 29E.2A3, #329718, BioLegend, 1:25), and APC-conjugated anti-CD86 (clone IT2.2, #305411, BioLegend, 1:50); (iv) PE-conjugated anti-CD204 (clone 7C9C20, #371904, BioLegend, 1:25) and APC-Cy7-conjugated anti-CD14 (clone MφP9, #557831, BD Pharmingen, 1:25).

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: Cord blood-derived natural killer cells engineered with a novel, highaffinity chimeric antigen receptor can eliminate acute myeloid leukemia cells with minimal C-type lectin-like molecule-1 expression.
    Article Snippet: .. PB, BM, and CB cells were stained with fluorochrome-conjugated mAbs after nonspecific binding was blocked by incubation with Human Serum AB (Gemini Bio) and FcR blocking reagent (Miltenyi Biotech). ..

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: PCR products were run on a 2% agarose gel (A42135, Invitrogen). .. For flow cytometry, cells were blocked with 1:300 human FcR Blocking Reagent (130-059-901, Miltenyi) for 15 minutes at 4°C, washed in FACS Buffer (PBS with 1% FCS and 2 mM EDTA), and stained with antibodies for 60 minutes in the presence of human FcR Blocking Reagent at 4°C (antibodies indicated in ). .. Cells were recorded on a BD LSR Fortessa, a Cytek Aurora, or a BD MacsQuant instrument and analysed with FlowJo (v10) or OMIQ (Dotmatics).

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).

    Incubation:

    Article Title: Endothelial senescence impairs efferocytosis and alters macrophage responses: insights into APP-CD74 signaling
    Article Snippet: .. Prior to staining, cells were incubated for 10 min at 4 °C with human FcR Blocking Reagent (#130-059-901, Miltenyi Biotec). .. Subsequently, the cells were aliquoted and stained for 30 min on ice and protected from light with the following antibody panels: (i), PE-Cy7-conjugated anti-CD169 (clone 7-239, #346014, BioLegend, 1:25), and APC-conjugated anti-HLA-DR (clone G46-6, #559866, BD Pharmingen, 1:10); (ii) AF488-conjugated anti-CD206 (clone 15-2, #321114, BioLegend, 1:20); (iii) FITC-conjugated anti-CD11b (clone ICRF44, #301330, BioLegend, 1:25), PE-conjugated anti-CD163 (clone GHI/61, #556018, BD Pharmingen, 1:10), PE-Cy7-conjugated anti-PD-L1 (clone 29E.2A3, #329718, BioLegend, 1:25), and APC-conjugated anti-CD86 (clone IT2.2, #305411, BioLegend, 1:50); (iv) PE-conjugated anti-CD204 (clone 7C9C20, #371904, BioLegend, 1:25) and APC-Cy7-conjugated anti-CD14 (clone MφP9, #557831, BD Pharmingen, 1:25).

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: Cord blood-derived natural killer cells engineered with a novel, highaffinity chimeric antigen receptor can eliminate acute myeloid leukemia cells with minimal C-type lectin-like molecule-1 expression.
    Article Snippet: .. PB, BM, and CB cells were stained with fluorochrome-conjugated mAbs after nonspecific binding was blocked by incubation with Human Serum AB (Gemini Bio) and FcR blocking reagent (Miltenyi Biotech). ..

    Article Title: Comparative profiling of MAIT cells across gastrointestinal cancers identifies conserved NK receptor dysregulation and HCC-specific defects in cytotoxicity and XBP1s signaling
    Article Snippet: .. Cells were incubated with 5 μL FcR Blocking Reagent (Miltenyi Biotec, REF: 130-059-901) and 2 μL Vα7.2-APC antibody (1:50; BioLegend, REF: 351708) for 15 minutes on ice. .. After washing with 4 mL PBS/BSA and centrifugation (300 × g, 6 minutes), cells were resuspended in 120 μL PBS/BSA and incubated with 30 μL APC-conjugated Nanobeads (Miltenyi Biotec, REF: 130-090-855) for 20 minutes at 4°C.

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Blocking Assay:

    Article Title: Endothelial senescence impairs efferocytosis and alters macrophage responses: insights into APP-CD74 signaling
    Article Snippet: .. Prior to staining, cells were incubated for 10 min at 4 °C with human FcR Blocking Reagent (#130-059-901, Miltenyi Biotec). .. Subsequently, the cells were aliquoted and stained for 30 min on ice and protected from light with the following antibody panels: (i), PE-Cy7-conjugated anti-CD169 (clone 7-239, #346014, BioLegend, 1:25), and APC-conjugated anti-HLA-DR (clone G46-6, #559866, BD Pharmingen, 1:10); (ii) AF488-conjugated anti-CD206 (clone 15-2, #321114, BioLegend, 1:20); (iii) FITC-conjugated anti-CD11b (clone ICRF44, #301330, BioLegend, 1:25), PE-conjugated anti-CD163 (clone GHI/61, #556018, BD Pharmingen, 1:10), PE-Cy7-conjugated anti-PD-L1 (clone 29E.2A3, #329718, BioLegend, 1:25), and APC-conjugated anti-CD86 (clone IT2.2, #305411, BioLegend, 1:50); (iv) PE-conjugated anti-CD204 (clone 7C9C20, #371904, BioLegend, 1:25) and APC-Cy7-conjugated anti-CD14 (clone MφP9, #557831, BD Pharmingen, 1:25).

    Article Title: A single domain intrabody biases the follicle-stimulating hormone receptor subcellular trafficking
    Article Snippet: Cells were detached following 1 h-incubation in Hanks Balanced Salt Solution (HBSS) w/o Ca2+ and Mg2+ (Eurobio, Les Ulis, France), and re-suspended in working buffer (PBS w/o Ca2+ and Mg2+ , 0.5% BSA) 48 h after transfection. .. Cells were incubated with FcR Blocking Reagent human (1:20, Miltenyi Biotech, Bergisch Gladbach, Germany) for 10 min at 4 °C, and with Phycoerythrin-conjugated anti-FLAG antibody (DYKDDDDK Antibody PE, 1:1000, Miltenyi Biotech, Bergisch Gladbach, Germany) and LIVE/DEAD Fixable Violet Dead Cell Stain (1:1000, Invitrogen, Waltham, MA, USA) for 30 min at 4 °C. .. Cells were washed twice in working buffer before permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization kit (BD Biosciences, Franklin Lakes, NJ, USA) following manufacturer’s protocol.

    Article Title: Cord blood-derived natural killer cells engineered with a novel, highaffinity chimeric antigen receptor can eliminate acute myeloid leukemia cells with minimal C-type lectin-like molecule-1 expression.
    Article Snippet: .. PB, BM, and CB cells were stained with fluorochrome-conjugated mAbs after nonspecific binding was blocked by incubation with Human Serum AB (Gemini Bio) and FcR blocking reagent (Miltenyi Biotech). ..

    Article Title: Comparative profiling of MAIT cells across gastrointestinal cancers identifies conserved NK receptor dysregulation and HCC-specific defects in cytotoxicity and XBP1s signaling
    Article Snippet: .. Cells were incubated with 5 μL FcR Blocking Reagent (Miltenyi Biotec, REF: 130-059-901) and 2 μL Vα7.2-APC antibody (1:50; BioLegend, REF: 351708) for 15 minutes on ice. .. After washing with 4 mL PBS/BSA and centrifugation (300 × g, 6 minutes), cells were resuspended in 120 μL PBS/BSA and incubated with 30 μL APC-conjugated Nanobeads (Miltenyi Biotec, REF: 130-090-855) for 20 minutes at 4°C.

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: PCR products were run on a 2% agarose gel (A42135, Invitrogen). .. For flow cytometry, cells were blocked with 1:300 human FcR Blocking Reagent (130-059-901, Miltenyi) for 15 minutes at 4°C, washed in FACS Buffer (PBS with 1% FCS and 2 mM EDTA), and stained with antibodies for 60 minutes in the presence of human FcR Blocking Reagent at 4°C (antibodies indicated in ). .. Cells were recorded on a BD LSR Fortessa, a Cytek Aurora, or a BD MacsQuant instrument and analysed with FlowJo (v10) or OMIQ (Dotmatics).

    Article Title: Exploratory analysis of early changes in peripheral immune cell proportions and clinical response following methotrexate and biologic treatment in rheumatoid arthritis
    Article Snippet: .. To prevent nonspecific antibody binding to immune cells, Fc receptors were blocked by incubating the cells with 400 μL human FcR Blocking Reagent (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) for 30 minutes at 4°C in the dark. ..

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Nonspecific Binding:

    Article Title: Cord blood-derived natural killer cells engineered with a novel, highaffinity chimeric antigen receptor can eliminate acute myeloid leukemia cells with minimal C-type lectin-like molecule-1 expression.
    Article Snippet: .. PB, BM, and CB cells were stained with fluorochrome-conjugated mAbs after nonspecific binding was blocked by incubation with Human Serum AB (Gemini Bio) and FcR blocking reagent (Miltenyi Biotech). ..

    Article Title: A multi-center, single-arm, phase 2 trial of dasatinib in T follicular helper cell lymphomas
    Article Snippet: .. Fc receptor-mediated nonspecific binding was blocked using the FcR Blocking Reagent (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining with 7- amino-actinomycin D (Thermo Fisher Scientific, A1310). .. FCM sorting and analysis were performed using BD FACSAria III (BD Biosciences), and data were analyzed using the FlowJo software (v10.7.1, Tree Star Inc.).

    Flow Cytometry:

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: PCR products were run on a 2% agarose gel (A42135, Invitrogen). .. For flow cytometry, cells were blocked with 1:300 human FcR Blocking Reagent (130-059-901, Miltenyi) for 15 minutes at 4°C, washed in FACS Buffer (PBS with 1% FCS and 2 mM EDTA), and stained with antibodies for 60 minutes in the presence of human FcR Blocking Reagent at 4°C (antibodies indicated in ). .. Cells were recorded on a BD LSR Fortessa, a Cytek Aurora, or a BD MacsQuant instrument and analysed with FlowJo (v10) or OMIQ (Dotmatics).

    FACS:

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: PCR products were run on a 2% agarose gel (A42135, Invitrogen). .. For flow cytometry, cells were blocked with 1:300 human FcR Blocking Reagent (130-059-901, Miltenyi) for 15 minutes at 4°C, washed in FACS Buffer (PBS with 1% FCS and 2 mM EDTA), and stained with antibodies for 60 minutes in the presence of human FcR Blocking Reagent at 4°C (antibodies indicated in ). .. Cells were recorded on a BD LSR Fortessa, a Cytek Aurora, or a BD MacsQuant instrument and analysed with FlowJo (v10) or OMIQ (Dotmatics).

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).

    Binding Assay:

    Article Title: Exploratory analysis of early changes in peripheral immune cell proportions and clinical response following methotrexate and biologic treatment in rheumatoid arthritis
    Article Snippet: .. To prevent nonspecific antibody binding to immune cells, Fc receptors were blocked by incubating the cells with 400 μL human FcR Blocking Reagent (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) for 30 minutes at 4°C in the dark. ..

    Red Blood Cell Lysis:

    Article Title: Activated protein C promotes hematopoietic stem and progenitor cell quiescence and engraftment.
    Article Snippet: Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Red blood cells (RBCs) were lysed by a 5-min incubation at RT in 1 mL of RBC lysis buffer (0.15 M NH4Cl, 0.01 M Tris-HCl, pH 7.5) and samples washed (300×g, 5 min, 4 °C) and resuspended in 500 μL of FACS buffer supplemented with 1:100 human FcR blocking reagent (Miltenyi Biotech #130-059-901) and 5% normal mouse (Invitrogen #50-112-1171) and 5% normal rat (Invitrogen #10-710-C) serum. .. Cells were all acquired at the CytoFLEX LX (Beckman Coulter).



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