Control:Article Title: Natural maternal immunity protects neonates from Escherichia coli sepsis.
Article Snippet: .. For some experiments, the GFP+ E. coli serum mixture was supplemented with rat anti-mouse CD16/32 (clone 2.4G2; BioXcell BE0307 (RRID AB_2736987)) or rat IgG2b kappa isotype control (clone LTF.2; BioXcell BE0090 (RRID AB_1107780)). .. For opsonization by human sera or blood spot specimens, THP1 activated macrophage (ATCC TIB-202) cells (1 × 104 cells per well), cells were seeded into 384-well tissue culture treated plates (Corning, 3701) to >80% confluency in DMEM medium supplemented with recombinant human TGFβ1 (1 ng ml−1; R&D, 7754-BH) and 1α,25-dihydroxyvitamin D3 (50 nM; Millipore Sigma, 705942) for 24 h. Adherent cells were then stimulated with phorbol-myristate acetate (15 ng ml−1; Sigma-Aldrich) for 15 min for macrophage differentiation as described84.
Staining:Article Title: IFITM3 deficiency drives SARS-CoV-2 adaptation while preserving variant-specific traits.
Article Snippet: Red blood cells were lysed using ACK lysis buffer (Gibco-Life Technologies). .. For staining, single-cell suspensions were resuspended in a 96-well plate, then a viability dye was added for 15 min at 4 C, followed by Fc receptor blocking buffer (anti-mouse CD16/CD32, Clone 2.4G2, BioXCell) for 15 min at 4 C. Cells were then incubated with anti-CCR2 antibody for 30 min at room temperature. .. Subsequently, a master mix of fluorochrome-conjugated antibodies diluted in FACS buffer was added for 40 min at 4 C (Myeloid panel cocktail: Viability - Violet 510 (Tonbo, 13-0870-T100), CD45 - APC-R700 (BD, 565478), CD11b – APC eFluor 780 (eBioscience, 47-0112-82), CD11c – APC (Invitrogen, 17-0114-81), Ly6G – PE-Cy7 (Biolegend, 127618), MHCII – eFluor 450 (eBioscience, 48-5321-82), CD103 – BUV737 (BD, 749393), CD64a – BUV 786 (BD, 569507), SiglecF – BB515 (BD, 564514)).
Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Article Title: Allergic lung inflammation can be modulated by niacin and drives the development of glycolytic eosinophils.
Article Snippet: Eosinophils are key contributors to allergic pathology, however, increasingly eosinophils are described to have important roles in organ health and immunoregulation.. The factors that define and regulate these diverse eosinophil functions remain poorly understood.. Here we show the emergence of a lung-specific Siglec-Fhi inflammatory eosinophil subset that exhibits high glycolytic activity, mTOR activation and increased amino acid and glucose uptake.
Article Title: Diverse Microbial Exposure Enhances CD8 + T Cell Effector Memory Output and Function
Article Snippet: .. For each sample, 500-5000K DAPI - , CD45 + , CD3ε + (IGT44) DAPI - , or CD45 + , tetramer + (IGT45) T cells were sorted on a FACS Aria II and pooled in a single collection tube at 4C. immgenT TotalSeq-C custom mouse panel staining: The pooled single cell suspensions were stained with the immgenT TotalSeq-C custom mouse panel, containing 128 antibodies (BioLegend Part no. 900004815) (see NCBI GEO SuperSeries accession number GSE297097 Supplementary Data for complete list) and FcBlock (Bio X Cell #BE0307). ..
Article Title: TCR Signal Strength and Eomes Coordinate CD8 + T Cell Fate and Antitumor Immunity in Hepatocellular Carcinoma
Article Snippet: .. Single-cell suspensions were stained with live/dead fixable near-IR dead cell stain kit (Thermo Fisher Scientific, L34976) or iFluor 860 maleimide (AAT Bioquest, 1408) to exclude dead cells, incubated with Fc receptor–blocking antibodies CD16/32 (clone 2.4G2, BioXcell, BE0307, RRID:AB_2736987) to block non-specific binding and stained with the following fluorochrome-conjugated antibodies for surface markers: CD45.1 (A20, 565212, RRID:AB_2722493) TCRβ (H57-597, 560657, RRID:AB_1727575), CD8α (53-6.7, 563068, RRID:AB_2687548), CD69 (H1.2F3, 612793, RRID:AB_2870120), CD38 (90/CD38, 562768, RRID:AB_2737781), CD39 (Duha59, 143805, RRID:AB_2563393), KLRG1 (2F1, 565393, RRID:AB_2739216) Tigit (1G9, 744213, RRID:AB_2742062), TIM-3 (5D12/TIM-3, 747621, RRID:AB_2744187), PD-1 (J43, 562523, RRID:AB_2737634), CD3 (17A2, 565642, RRID:AB_2739318), CX3CR1 (Z8-50, 567820, RRID:AB_2916747), CXCR6 (SA051D1, 151109, RRID: AB_2616760), CD49a (Ha31/8, 740519, RRID:AB_2740235), H2-K b SIINFEKL -PE pentamer (F093-2B, PRO-Immune). .. For detecting intranuclear proteins, cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) before staining with the following antibodies: Eomes (Dan11mag, 53-4875-82, RRID:AB_10854265), TCF1 (S33-966, 564217, RRID:AB_2687845) and TOX (TXRX10, 50-6502-82, RRID:AB_2574265) from Thermo Fisher Scientific.
Article Title: immgenT: A Comprehensive Reference of Convergent T-cell States in the Mouse
Article Snippet: .. A lyophilized tube of ImmGenT TotalSeq-C custom mouse panel, containing 128 antibodies (Biolegend Part no. 900004815, panel information detailed in Extended Data Table 2) was equilibrated to room temperature for 5min; centrifugated for 30sec at 10,000g; resuspended with 24uL of staining buffer (described above) and 1uL of FcBlock (Bio X Cell #BE0307); and incubated at room temperature for 30min. ..
Single Cell:Article Title: IFITM3 deficiency drives SARS-CoV-2 adaptation while preserving variant-specific traits.
Article Snippet: Red blood cells were lysed using ACK lysis buffer (Gibco-Life Technologies). .. For staining, single-cell suspensions were resuspended in a 96-well plate, then a viability dye was added for 15 min at 4 C, followed by Fc receptor blocking buffer (anti-mouse CD16/CD32, Clone 2.4G2, BioXCell) for 15 min at 4 C. Cells were then incubated with anti-CCR2 antibody for 30 min at room temperature. .. Subsequently, a master mix of fluorochrome-conjugated antibodies diluted in FACS buffer was added for 40 min at 4 C (Myeloid panel cocktail: Viability - Violet 510 (Tonbo, 13-0870-T100), CD45 - APC-R700 (BD, 565478), CD11b – APC eFluor 780 (eBioscience, 47-0112-82), CD11c – APC (Invitrogen, 17-0114-81), Ly6G – PE-Cy7 (Biolegend, 127618), MHCII – eFluor 450 (eBioscience, 48-5321-82), CD103 – BUV737 (BD, 749393), CD64a – BUV 786 (BD, 569507), SiglecF – BB515 (BD, 564514)).
Article Title: Diverse Microbial Exposure Enhances CD8 + T Cell Effector Memory Output and Function
Article Snippet: .. For each sample, 500-5000K DAPI - , CD45 + , CD3ε + (IGT44) DAPI - , or CD45 + , tetramer + (IGT45) T cells were sorted on a FACS Aria II and pooled in a single collection tube at 4C. immgenT TotalSeq-C custom mouse panel staining: The pooled single cell suspensions were stained with the immgenT TotalSeq-C custom mouse panel, containing 128 antibodies (BioLegend Part no. 900004815) (see NCBI GEO SuperSeries accession number GSE297097 Supplementary Data for complete list) and FcBlock (Bio X Cell #BE0307). ..
Article Title: TCR Signal Strength and Eomes Coordinate CD8 + T Cell Fate and Antitumor Immunity in Hepatocellular Carcinoma
Article Snippet: .. Single-cell suspensions were stained with live/dead fixable near-IR dead cell stain kit (Thermo Fisher Scientific, L34976) or iFluor 860 maleimide (AAT Bioquest, 1408) to exclude dead cells, incubated with Fc receptor–blocking antibodies CD16/32 (clone 2.4G2, BioXcell, BE0307, RRID:AB_2736987) to block non-specific binding and stained with the following fluorochrome-conjugated antibodies for surface markers: CD45.1 (A20, 565212, RRID:AB_2722493) TCRβ (H57-597, 560657, RRID:AB_1727575), CD8α (53-6.7, 563068, RRID:AB_2687548), CD69 (H1.2F3, 612793, RRID:AB_2870120), CD38 (90/CD38, 562768, RRID:AB_2737781), CD39 (Duha59, 143805, RRID:AB_2563393), KLRG1 (2F1, 565393, RRID:AB_2739216) Tigit (1G9, 744213, RRID:AB_2742062), TIM-3 (5D12/TIM-3, 747621, RRID:AB_2744187), PD-1 (J43, 562523, RRID:AB_2737634), CD3 (17A2, 565642, RRID:AB_2739318), CX3CR1 (Z8-50, 567820, RRID:AB_2916747), CXCR6 (SA051D1, 151109, RRID: AB_2616760), CD49a (Ha31/8, 740519, RRID:AB_2740235), H2-K b SIINFEKL -PE pentamer (F093-2B, PRO-Immune). .. For detecting intranuclear proteins, cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) before staining with the following antibodies: Eomes (Dan11mag, 53-4875-82, RRID:AB_10854265), TCF1 (S33-966, 564217, RRID:AB_2687845) and TOX (TXRX10, 50-6502-82, RRID:AB_2574265) from Thermo Fisher Scientific.
Blocking Assay:Article Title: IFITM3 deficiency drives SARS-CoV-2 adaptation while preserving variant-specific traits.
Article Snippet: Red blood cells were lysed using ACK lysis buffer (Gibco-Life Technologies). .. For staining, single-cell suspensions were resuspended in a 96-well plate, then a viability dye was added for 15 min at 4 C, followed by Fc receptor blocking buffer (anti-mouse CD16/CD32, Clone 2.4G2, BioXCell) for 15 min at 4 C. Cells were then incubated with anti-CCR2 antibody for 30 min at room temperature. .. Subsequently, a master mix of fluorochrome-conjugated antibodies diluted in FACS buffer was added for 40 min at 4 C (Myeloid panel cocktail: Viability - Violet 510 (Tonbo, 13-0870-T100), CD45 - APC-R700 (BD, 565478), CD11b – APC eFluor 780 (eBioscience, 47-0112-82), CD11c – APC (Invitrogen, 17-0114-81), Ly6G – PE-Cy7 (Biolegend, 127618), MHCII – eFluor 450 (eBioscience, 48-5321-82), CD103 – BUV737 (BD, 749393), CD64a – BUV 786 (BD, 569507), SiglecF – BB515 (BD, 564514)).
Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Article Title: TCR Signal Strength and Eomes Coordinate CD8 + T Cell Fate and Antitumor Immunity in Hepatocellular Carcinoma
Article Snippet: .. Single-cell suspensions were stained with live/dead fixable near-IR dead cell stain kit (Thermo Fisher Scientific, L34976) or iFluor 860 maleimide (AAT Bioquest, 1408) to exclude dead cells, incubated with Fc receptor–blocking antibodies CD16/32 (clone 2.4G2, BioXcell, BE0307, RRID:AB_2736987) to block non-specific binding and stained with the following fluorochrome-conjugated antibodies for surface markers: CD45.1 (A20, 565212, RRID:AB_2722493) TCRβ (H57-597, 560657, RRID:AB_1727575), CD8α (53-6.7, 563068, RRID:AB_2687548), CD69 (H1.2F3, 612793, RRID:AB_2870120), CD38 (90/CD38, 562768, RRID:AB_2737781), CD39 (Duha59, 143805, RRID:AB_2563393), KLRG1 (2F1, 565393, RRID:AB_2739216) Tigit (1G9, 744213, RRID:AB_2742062), TIM-3 (5D12/TIM-3, 747621, RRID:AB_2744187), PD-1 (J43, 562523, RRID:AB_2737634), CD3 (17A2, 565642, RRID:AB_2739318), CX3CR1 (Z8-50, 567820, RRID:AB_2916747), CXCR6 (SA051D1, 151109, RRID: AB_2616760), CD49a (Ha31/8, 740519, RRID:AB_2740235), H2-K b SIINFEKL -PE pentamer (F093-2B, PRO-Immune). .. For detecting intranuclear proteins, cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) before staining with the following antibodies: Eomes (Dan11mag, 53-4875-82, RRID:AB_10854265), TCF1 (S33-966, 564217, RRID:AB_2687845) and TOX (TXRX10, 50-6502-82, RRID:AB_2574265) from Thermo Fisher Scientific.
Incubation:Article Title: IFITM3 deficiency drives SARS-CoV-2 adaptation while preserving variant-specific traits.
Article Snippet: Red blood cells were lysed using ACK lysis buffer (Gibco-Life Technologies). .. For staining, single-cell suspensions were resuspended in a 96-well plate, then a viability dye was added for 15 min at 4 C, followed by Fc receptor blocking buffer (anti-mouse CD16/CD32, Clone 2.4G2, BioXCell) for 15 min at 4 C. Cells were then incubated with anti-CCR2 antibody for 30 min at room temperature. .. Subsequently, a master mix of fluorochrome-conjugated antibodies diluted in FACS buffer was added for 40 min at 4 C (Myeloid panel cocktail: Viability - Violet 510 (Tonbo, 13-0870-T100), CD45 - APC-R700 (BD, 565478), CD11b – APC eFluor 780 (eBioscience, 47-0112-82), CD11c – APC (Invitrogen, 17-0114-81), Ly6G – PE-Cy7 (Biolegend, 127618), MHCII – eFluor 450 (eBioscience, 48-5321-82), CD103 – BUV737 (BD, 749393), CD64a – BUV 786 (BD, 569507), SiglecF – BB515 (BD, 564514)).
Article Title: TCR Signal Strength and Eomes Coordinate CD8 + T Cell Fate and Antitumor Immunity in Hepatocellular Carcinoma
Article Snippet: .. Single-cell suspensions were stained with live/dead fixable near-IR dead cell stain kit (Thermo Fisher Scientific, L34976) or iFluor 860 maleimide (AAT Bioquest, 1408) to exclude dead cells, incubated with Fc receptor–blocking antibodies CD16/32 (clone 2.4G2, BioXcell, BE0307, RRID:AB_2736987) to block non-specific binding and stained with the following fluorochrome-conjugated antibodies for surface markers: CD45.1 (A20, 565212, RRID:AB_2722493) TCRβ (H57-597, 560657, RRID:AB_1727575), CD8α (53-6.7, 563068, RRID:AB_2687548), CD69 (H1.2F3, 612793, RRID:AB_2870120), CD38 (90/CD38, 562768, RRID:AB_2737781), CD39 (Duha59, 143805, RRID:AB_2563393), KLRG1 (2F1, 565393, RRID:AB_2739216) Tigit (1G9, 744213, RRID:AB_2742062), TIM-3 (5D12/TIM-3, 747621, RRID:AB_2744187), PD-1 (J43, 562523, RRID:AB_2737634), CD3 (17A2, 565642, RRID:AB_2739318), CX3CR1 (Z8-50, 567820, RRID:AB_2916747), CXCR6 (SA051D1, 151109, RRID: AB_2616760), CD49a (Ha31/8, 740519, RRID:AB_2740235), H2-K b SIINFEKL -PE pentamer (F093-2B, PRO-Immune). .. For detecting intranuclear proteins, cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) before staining with the following antibodies: Eomes (Dan11mag, 53-4875-82, RRID:AB_10854265), TCF1 (S33-966, 564217, RRID:AB_2687845) and TOX (TXRX10, 50-6502-82, RRID:AB_2574265) from Thermo Fisher Scientific.
Article Title: immgenT: A Comprehensive Reference of Convergent T-cell States in the Mouse
Article Snippet: .. A lyophilized tube of ImmGenT TotalSeq-C custom mouse panel, containing 128 antibodies (Biolegend Part no. 900004815, panel information detailed in Extended Data Table 2) was equilibrated to room temperature for 5min; centrifugated for 30sec at 10,000g; resuspended with 24uL of staining buffer (described above) and 1uL of FcBlock (Bio X Cell #BE0307); and incubated at room temperature for 30min. ..
Purification:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Recombinant:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Enzyme-linked Immunospot:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Flow Cytometry:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Selection:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Virus:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Expressing:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
FACS:Article Title: Diverse Microbial Exposure Enhances CD8 + T Cell Effector Memory Output and Function
Article Snippet: .. For each sample, 500-5000K DAPI - , CD45 + , CD3ε + (IGT44) DAPI - , or CD45 + , tetramer + (IGT45) T cells were sorted on a FACS Aria II and pooled in a single collection tube at 4C. immgenT TotalSeq-C custom mouse panel staining: The pooled single cell suspensions were stained with the immgenT TotalSeq-C custom mouse panel, containing 128 antibodies (BioLegend Part no. 900004815) (see NCBI GEO SuperSeries accession number GSE297097 Supplementary Data for complete list) and FcBlock (Bio X Cell #BE0307). ..
Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease
Article Snippet: To assess the effect of Sitagliptin on the differentiation of donor-derived CD8+ T cells in vivo, peripheral blood and spleens were harvested from recipient mice on day 7 post-transplantation and dissociated through a 40-μm cell strainer in RPMI 1640 medium containing 20 mg/mL BSA (Thermo Fisher Scientific). .. After red blood cell lysis with RBC Lysis Buffer and washing with FACS buffer, cells were blocked using anti-mouse CD16/CD32 antibody (1 μg/mL, BE0307, BioXcell) for 10 min at 4 °C to prevent nonspecific binding. .. Surface staining was then performed with the following fluorochrome-conjugated antibodies: Fixable Viability Dye (FVD, eFluor 506, eBioscience) to exclude dead cells, BUV661 anti-H2k d (1 μg/mL, BioLegend), APC-Cy7 anti-CD45 (1 μg/mL, BioLegend), PE-Cy5 anti-CD3 (1 μg/mL, BioLegend), SB702 anti-CD4 (1 μg/mL, RM4-5), PE-Cy5.5 anti-CD8 (1 μg/mL, 53-6.7), PE-Cy7 anti-PD-1 (1 μg/mL, RMP1-30) and APC anti-Tim-3 (1 μg/mL, RMT3-23).
Binding Assay:Article Title: TCR Signal Strength and Eomes Coordinate CD8 + T Cell Fate and Antitumor Immunity in Hepatocellular Carcinoma
Article Snippet: .. Single-cell suspensions were stained with live/dead fixable near-IR dead cell stain kit (Thermo Fisher Scientific, L34976) or iFluor 860 maleimide (AAT Bioquest, 1408) to exclude dead cells, incubated with Fc receptor–blocking antibodies CD16/32 (clone 2.4G2, BioXcell, BE0307, RRID:AB_2736987) to block non-specific binding and stained with the following fluorochrome-conjugated antibodies for surface markers: CD45.1 (A20, 565212, RRID:AB_2722493) TCRβ (H57-597, 560657, RRID:AB_1727575), CD8α (53-6.7, 563068, RRID:AB_2687548), CD69 (H1.2F3, 612793, RRID:AB_2870120), CD38 (90/CD38, 562768, RRID:AB_2737781), CD39 (Duha59, 143805, RRID:AB_2563393), KLRG1 (2F1, 565393, RRID:AB_2739216) Tigit (1G9, 744213, RRID:AB_2742062), TIM-3 (5D12/TIM-3, 747621, RRID:AB_2744187), PD-1 (J43, 562523, RRID:AB_2737634), CD3 (17A2, 565642, RRID:AB_2739318), CX3CR1 (Z8-50, 567820, RRID:AB_2916747), CXCR6 (SA051D1, 151109, RRID: AB_2616760), CD49a (Ha31/8, 740519, RRID:AB_2740235), H2-K b SIINFEKL -PE pentamer (F093-2B, PRO-Immune). .. For detecting intranuclear proteins, cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) before staining with the following antibodies: Eomes (Dan11mag, 53-4875-82, RRID:AB_10854265), TCF1 (S33-966, 564217, RRID:AB_2687845) and TOX (TXRX10, 50-6502-82, RRID:AB_2574265) from Thermo Fisher Scientific.
Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease
Article Snippet: To assess the effect of Sitagliptin on the differentiation of donor-derived CD8+ T cells in vivo, peripheral blood and spleens were harvested from recipient mice on day 7 post-transplantation and dissociated through a 40-μm cell strainer in RPMI 1640 medium containing 20 mg/mL BSA (Thermo Fisher Scientific). .. After red blood cell lysis with RBC Lysis Buffer and washing with FACS buffer, cells were blocked using anti-mouse CD16/CD32 antibody (1 μg/mL, BE0307, BioXcell) for 10 min at 4 °C to prevent nonspecific binding. .. Surface staining was then performed with the following fluorochrome-conjugated antibodies: Fixable Viability Dye (FVD, eFluor 506, eBioscience) to exclude dead cells, BUV661 anti-H2k d (1 μg/mL, BioLegend), APC-Cy7 anti-CD45 (1 μg/mL, BioLegend), PE-Cy5 anti-CD3 (1 μg/mL, BioLegend), SB702 anti-CD4 (1 μg/mL, RM4-5), PE-Cy5.5 anti-CD8 (1 μg/mL, 53-6.7), PE-Cy7 anti-PD-1 (1 μg/mL, RMP1-30) and APC anti-Tim-3 (1 μg/mL, RMT3-23).
Red Blood Cell Lysis:Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease
Article Snippet: To assess the effect of Sitagliptin on the differentiation of donor-derived CD8+ T cells in vivo, peripheral blood and spleens were harvested from recipient mice on day 7 post-transplantation and dissociated through a 40-μm cell strainer in RPMI 1640 medium containing 20 mg/mL BSA (Thermo Fisher Scientific). .. After red blood cell lysis with RBC Lysis Buffer and washing with FACS buffer, cells were blocked using anti-mouse CD16/CD32 antibody (1 μg/mL, BE0307, BioXcell) for 10 min at 4 °C to prevent nonspecific binding. .. Surface staining was then performed with the following fluorochrome-conjugated antibodies: Fixable Viability Dye (FVD, eFluor 506, eBioscience) to exclude dead cells, BUV661 anti-H2k d (1 μg/mL, BioLegend), APC-Cy7 anti-CD45 (1 μg/mL, BioLegend), PE-Cy5 anti-CD3 (1 μg/mL, BioLegend), SB702 anti-CD4 (1 μg/mL, RM4-5), PE-Cy5.5 anti-CD8 (1 μg/mL, 53-6.7), PE-Cy7 anti-PD-1 (1 μg/mL, RMP1-30) and APC anti-Tim-3 (1 μg/mL, RMT3-23).
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