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facs buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc facs buffer
    Facs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer+dapi/DAPI/pm40316023-209-7-14
    Average 98 stars, based on 2157 article reviews
    facs buffer - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Metabolic‐Redox Remodeling of Inflammatory Synovium by Mannose‐Modified Selenium Nanoparticles for Osteoarthritis Therapy
    Article Snippet: .. Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100, and stained with Actin‐Tracker Green‐488 (1:200, Beyotime) and DAPI (1:1000, CST). .. Cellular internalization was observed via a Leica STELLARIS 5 confocal microscope and quantitatively assessed by flow cytometry (BD LSRFortessa) with DiD fluorescence measured in the APC channel.

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. ..

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: In brief, Adenoassociated virus (AAV) vector-mediated overexpression of mouse RUNX1, control (ZsGreen), Adenoassociated virus (AAV)-Cre-ZsGreen and Control (ZsGreen) were purchased from Hanbio biotechnology (Shanghai, China). .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature.

    other:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Sections were then incubated with primary antibodies against UCP1 (Abcam, ab10983), PPARγ (Santa Cruz Biotechnology, sc-7273), and PDGFRα (R&D Systems, AF-307-NA) at 4 °C overnight.

    Incubation:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).

    Blocking Assay:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).



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    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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    Image Search Results


    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Journal: bioRxiv

    Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

    doi: 10.1101/2025.04.25.650344

    Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Article Snippet: Cells were then washed with FACS buffer (1:5), and resuspended in 300μl FACS buffer + DAPI ((Miltenyi Biotec, # 130-111-570) for live/dead cell identification.

    Techniques: Immunostaining, Expressing, Staining