facs buffer (Cell Signaling Technology Inc)
Structured Review
Facs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer+dapi/DAPI/pm40316023-209-7-14
Average 98 stars, based on 2157 article reviews
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Staining:Article Title: Metabolic‐Redox Remodeling of Inflammatory Synovium by Mannose‐Modified Selenium Nanoparticles for Osteoarthritis Therapy Article Snippet: .. Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100, and stained with Actin‐Tracker Green‐488 (1:200, Beyotime) and Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: In brief, Adenoassociated virus (AAV) vector-mediated overexpression of mouse RUNX1, control (ZsGreen), Adenoassociated virus (AAV)-Cre-ZsGreen and Control (ZsGreen) were purchased from Hanbio biotechnology (Shanghai, China). .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL other:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: Sections were then incubated with primary antibodies against UCP1 (Abcam, ab10983), PPARγ (Santa Cruz Biotechnology, sc-7273), and PDGFRα (R&D Systems, AF-307-NA) at 4 °C overnight. Incubation:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Blocking Assay:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. |
