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pcmv6 entry  (OriGene)


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    Structured Review

    OriGene pcmv6 entry
    Pcmv6 Entry, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector/pCMV6-Entry+Mammalian+Expression+Vector/us12648946-908-9-12
    Average 96 stars, based on 1702 article reviews
    pcmv6 entry - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Mitochondrial calcium depletion in Darier disease is rescued by dantrolene sodium partly via MCU.
    Article Snippet: Simultaneously, universal negative (Sigma-Aldrich) was used for 24-hour to create siNEG cells. .. MCU OE was induced by MCU human-tagged ORF clone (NM_138357) (Origene), with pCMV6 entry vector (Origene) acting as a nontargeting control, both for 6 hours using Lipofectamine 3000 (Thermo Fisher Scientific) in serum-free OPTI-MEM media (Thermo Fisher Scientific). .. For the fluorescent measurement of mtCa2+, cell models were transfected with a Ca2+GFP reporter CMV-Mito4x-GCaMP6f (Addgene) localised to mitochondria for 24 hours using Lipofectamine 3000.

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling
    Article Snippet: Mouse Nrp1 Plasmid , Origene , Cat# MR211197. .. pCMV6-Entry Plasmid , Origene , Cat# PS100001. .. Mouse Aldh1a3 CRISPR Plasmid , Santa Cruz Biotechnology , Cat# sc-425322.

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. or overexpression studies in LUAD cell lines, the open reading rame (ORF) of CYYR1 was PCR-amplified and cloned into the CMV6-entry vector (Ori Gene Technologies, PS100001) to genrate the CYYR1-OE plasmid. ..


    Article Title: Mifepristone modulates response to CDK4/6 inhibition in progesterone receptor isoform A-high breast cancer models
    Article Snippet: .. E2F1 (NM_005225) human tagged ORF clone (Myc-DDK-tagged; PS100001) and the pCMV6-Entry mammalian expression vector (RC208247; vector backbone: pCMV6-Entry) were obtained from Origene. ..

    Control:

    Article Title: Mitochondrial calcium depletion in Darier disease is rescued by dantrolene sodium partly via MCU.
    Article Snippet: Simultaneously, universal negative (Sigma-Aldrich) was used for 24-hour to create siNEG cells. .. MCU OE was induced by MCU human-tagged ORF clone (NM_138357) (Origene), with pCMV6 entry vector (Origene) acting as a nontargeting control, both for 6 hours using Lipofectamine 3000 (Thermo Fisher Scientific) in serum-free OPTI-MEM media (Thermo Fisher Scientific). .. For the fluorescent measurement of mtCa2+, cell models were transfected with a Ca2+GFP reporter CMV-Mito4x-GCaMP6f (Addgene) localised to mitochondria for 24 hours using Lipofectamine 3000.

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Cell Culture:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Suspension:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Immunocytochemistry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Flow Cytometry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Western Blot:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Expressing:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Article Title: Mifepristone modulates response to CDK4/6 inhibition in progesterone receptor isoform A-high breast cancer models
    Article Snippet: .. E2F1 (NM_005225) human tagged ORF clone (Myc-DDK-tagged; PS100001) and the pCMV6-Entry mammalian expression vector (RC208247; vector backbone: pCMV6-Entry) were obtained from Origene. ..

    Construct:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Over Expression:

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. or overexpression studies in LUAD cell lines, the open reading rame (ORF) of CYYR1 was PCR-amplified and cloned into the CMV6-entry vector (Ori Gene Technologies, PS100001) to genrate the CYYR1-OE plasmid. ..

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Polymerase Chain Reaction:

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. or overexpression studies in LUAD cell lines, the open reading rame (ORF) of CYYR1 was PCR-amplified and cloned into the CMV6-entry vector (Ori Gene Technologies, PS100001) to genrate the CYYR1-OE plasmid. ..

    Clone Assay:

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. or overexpression studies in LUAD cell lines, the open reading rame (ORF) of CYYR1 was PCR-amplified and cloned into the CMV6-entry vector (Ori Gene Technologies, PS100001) to genrate the CYYR1-OE plasmid. ..

    Recombinant:


    Software:


    Transfection:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.



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    Image Search Results


    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: The cDNAs encoding the extracellular domains of the HLA-DR3 α- and β-chains were separately cloned into the pHLsec expression vector (Addgene plasmid #99845).

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software