pcmv6 (OriGene)
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pcmv6
Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector/pCMV6-AN-HA+Mammalian+Expression+Vector/pm22912588-307-7-8
Average 92 stars, based on 21 article reviews
Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector/pCMV6-AN-HA+Mammalian+Expression+Vector/pm22912588-307-7-8
Average 92 stars, based on 21 article reviews
pcmv6 - by Bioz Stars,
2026-10
92/100 stars
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Expressing:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: All synthetic DNA fragments were chemically synthesized by GenScript (Piscataway, NJ). .. Mammalian expression vectors: The wild-type FLAG-Pol i (pJRM46) and wild-type HA-Pol g (pJRM56) expression vectors has been described previously,32 as have the FLAG-Pol i 1-590 and 1- 420C-terminal truncations constructs.34 The wildtype HA-Pol i expression plasmid was created by subcloning the Pol i coding sequence from the wild-type FLAG-Pol i vector into the AsiSI and MluI restriction sites of Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Article Title: Solid tumor target-immunotherapy with novel biomimetic immuno- chemotherapy composite mesoporous silica nanoparticles coated by the CD80 modified cell membranes Article Snippet: .. The Article Title: Novel biomimetic mesoporous silica nanoparticles system possessed targetability and immune synergy to promote solid tumor immuno- chemotherapy efficacy Article Snippet: .. The chimeric CD80-LFA3 cDNA was ligated into Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: .. The coding sequences for RAD23A and RAD23B were synthesized and cloned into the AsiSI and HindIII restriction sites of Article Title: Astrocytes modulate brain phosphate homeostasis via polarized distribution of phosphate uptake transporter PiT2 and exporter XPR1. Article Snippet: Article Construct:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: All synthetic DNA fragments were chemically synthesized by GenScript (Piscataway, NJ). .. Mammalian expression vectors: The wild-type FLAG-Pol i (pJRM46) and wild-type HA-Pol g (pJRM56) expression vectors has been described previously,32 as have the FLAG-Pol i 1-590 and 1- 420C-terminal truncations constructs.34 The wildtype HA-Pol i expression plasmid was created by subcloning the Pol i coding sequence from the wild-type FLAG-Pol i vector into the AsiSI and MluI restriction sites of Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: .. The coding sequences for RAD23A and RAD23B were synthesized and cloned into the AsiSI and HindIII restriction sites of Article Title: Astrocytes modulate brain phosphate homeostasis via polarized distribution of phosphate uptake transporter PiT2 and exporter XPR1. Article Snippet: Article Plasmid Preparation:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: All synthetic DNA fragments were chemically synthesized by GenScript (Piscataway, NJ). .. Mammalian expression vectors: The wild-type FLAG-Pol i (pJRM46) and wild-type HA-Pol g (pJRM56) expression vectors has been described previously,32 as have the FLAG-Pol i 1-590 and 1- 420C-terminal truncations constructs.34 The wildtype HA-Pol i expression plasmid was created by subcloning the Pol i coding sequence from the wild-type FLAG-Pol i vector into the AsiSI and MluI restriction sites of Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Article Title: Solid tumor target-immunotherapy with novel biomimetic immuno- chemotherapy composite mesoporous silica nanoparticles coated by the CD80 modified cell membranes Article Snippet: .. The Article Title: Novel biomimetic mesoporous silica nanoparticles system possessed targetability and immune synergy to promote solid tumor immuno- chemotherapy efficacy Article Snippet: .. The chimeric CD80-LFA3 cDNA was ligated into Subcloning:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: All synthetic DNA fragments were chemically synthesized by GenScript (Piscataway, NJ). .. Mammalian expression vectors: The wild-type FLAG-Pol i (pJRM46) and wild-type HA-Pol g (pJRM56) expression vectors has been described previously,32 as have the FLAG-Pol i 1-590 and 1- 420C-terminal truncations constructs.34 The wildtype HA-Pol i expression plasmid was created by subcloning the Pol i coding sequence from the wild-type FLAG-Pol i vector into the AsiSI and MluI restriction sites of Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Sequencing:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: All synthetic DNA fragments were chemically synthesized by GenScript (Piscataway, NJ). .. Mammalian expression vectors: The wild-type FLAG-Pol i (pJRM46) and wild-type HA-Pol g (pJRM56) expression vectors has been described previously,32 as have the FLAG-Pol i 1-590 and 1- 420C-terminal truncations constructs.34 The wildtype HA-Pol i expression plasmid was created by subcloning the Pol i coding sequence from the wild-type FLAG-Pol i vector into the AsiSI and MluI restriction sites of Article Title: Identification of B56α, B56γ and BAP1 as PRR14L binding partners Article Snippet: .. Both were inserted into Generated:Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Polymerase Chain Reaction:Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Ubiquitin Proteomics:Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the FLAG-tag:Article Title: E3 ubiquitin ligase RNF2 protects polymerase ι from destabilization. Article Snippet: Plasmid pJRM70, expressing GST-RNF2 recombinant protein was generated by sub-cloning the synthesized RNF2 gene (Genscript) into the pGEX-4T-1 vector. pT3-EF1a-Bmi1 was a gift from Xin Chen (Addgene plasmid # 31783; http://n2t.net/addgene:31783; RRID: Addgene_31783) [34]. .. BMI1 C-terminally tagged with HA epitope was subcloned into pGEX-4T-1 to create pMF4 expressing BMI1 N-terminally tagged with GST and C-terminally tagged HA. pMF10 expressing N-terminally HA-tagged full-length wild-type RNF2 protein was generated by subcloning respective PCR fragments into the Hemagglutination Assay:Article Title: Solid tumor target-immunotherapy with novel biomimetic immuno- chemotherapy composite mesoporous silica nanoparticles coated by the CD80 modified cell membranes Article Snippet: .. The Article Title: Novel biomimetic mesoporous silica nanoparticles system possessed targetability and immune synergy to promote solid tumor immuno- chemotherapy efficacy Article Snippet: .. The chimeric CD80-LFA3 cDNA was ligated into Synthesized:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: .. The coding sequences for RAD23A and RAD23B were synthesized and cloned into the AsiSI and HindIII restriction sites of Clone Assay:Article Title: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. Article Snippet: .. The coding sequences for RAD23A and RAD23B were synthesized and cloned into the AsiSI and HindIII restriction sites of Article Title: Astrocytes modulate brain phosphate homeostasis via polarized distribution of phosphate uptake transporter PiT2 and exporter XPR1. Article Snippet: Article |
