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Promega equilibration buffer
Equilibration Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/equilibration+buffer/equilibration+buffer/pmc07423176-256-1-4
Average 90 stars, based on 1 article reviews
equilibration buffer - by Bioz Stars, 2026-09
90/100 stars

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Staining:

Article Title: Tumor necrosis factor-related apoptosis-inducing ligand modulates angiogenesis and apoptosis to inhibit non-small cell lung carcinoma tumor growth in mice
Article Snippet: .. Equilibration Buffer (100 μl) was added to the slides at room temperature for 5–10 minutes, and staining was performed using the TUNEL assay kit (Promega Systems, Madison, WI, USA). .. Apoptotic cells that stained pale brown were visualized at × 100 magnification under light microscopy (Olympus Optical Co., Tokyo, Japan) equipped with the Moticam 5000 C camera (Richmond, BC, Canada).

TUNEL Assay:

Article Title: Tumor necrosis factor-related apoptosis-inducing ligand modulates angiogenesis and apoptosis to inhibit non-small cell lung carcinoma tumor growth in mice
Article Snippet: .. Equilibration Buffer (100 μl) was added to the slides at room temperature for 5–10 minutes, and staining was performed using the TUNEL assay kit (Promega Systems, Madison, WI, USA). .. Apoptotic cells that stained pale brown were visualized at × 100 magnification under light microscopy (Olympus Optical Co., Tokyo, Japan) equipped with the Moticam 5000 C camera (Richmond, BC, Canada).

Article Title: Extracellular vesicles of bovine small follicular fluid promote ovarian cortical stromal cell proliferation and steroidogenesis.
Article Snippet: Extracellular vesicles (EVs) are membranestructured vesicles (S. Liu, Zhang, et al., 2019; Mantel & Marti, 2014; Oliveira et al., 2010) that contain a variety of biologically active molecules (Camussi et al., 2010; Willms et al., 2018).. In addition to proteins and lipids (especially high levels of sphingomyelin), EVs also contain DNA and various RNAs, including mRNA and miRNA (Chen et al., 2020; Fabbiano et al., 2020; Kim et al., 2017; T. Liu, Zhang, et al., 2019; Théry et al., 2009; Tsatsaronis et al., 2018).. Previous studies reported that EVs have been isolated from semen, epididymis, fallopian tube, uterine fluid and follicular fluid (AlDossary et al., 2013; Hung et al., 2015; Hung et al., 2017; Saez et al., 2003).

Article Title: Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s Disease
Article Snippet: .. The260 cells were then equilibrated at room temperature for261 10 minutes using 30 l of equilibration buffer pro-262 vided with Promega TUNEL Kit (Promega, DeadEnd263 Fluorometric TUNEL Assay, Cat# G3250). ..

Incubation:

Article Title: Antibacterial and anticancer activity of two NK-lysin-derived peptides from the Antarctic teleost Trematomus bernacchii.
Article Snippet: .. After that, cells were exposed to the equilibration buffer (Promega) for 10 min and, subsequently, incubated with the rTdT incubation buffer (Promega) at 37 ◦C for 1 h in the dark to allow the tailing of green fluorescent nucleotides to 3 –OH DNA ends. .. The reaction was stopped by adding the 2X SSC buffer (Promega) to the cells.

Article Title: Targeting Forward and Reverse EphB4/EFNB2 Signaling by a Peptide with Dual Functions
Article Snippet: Slides were subjected to recombinant terminal deoxynucleotidyl transferase (rTdT)–mediated dUTP nick-end labeling (TUNEL) by using the DeadEnd Fluorometric TUNEL System assay kit (Promega) after CD31 staining by immersing slides in 4% formaldehyde in PBS for 15 min. A 20 μg/mL proteinase K solution (Promega) was added to each slide (100 μL per slide), and the slides were incubated at room temperature for 8- 10 min. .. Slides were washed twice with PBS, 100 μL equilibration buffer (Promega) was added to each, and the slides were again incubated for 5-10 min. ..

Article Title: MicroRNA-34a-5p as a promising early circulating preclinical biomarker of doxorubicin-induced chronic cardiotoxicity.
Article Snippet: Received November 23, 1999; revision received March 23, 2000; accepted March 23, 2000.. BACKGROUND.. Recent studies have suggested that vascular endothelial growth factor (VEGF), in addition to its proangiogenic properties, also functions as a survival factor for endothelial cells.

Article Title: Photoreceptor Degeneration in Pro23His Transgenic Rats (Line 3) Involves Autophagic and Necroptotic Mechanisms
Article Snippet: To label dying cells, TUNEL assay (DeadEnd TM Fluorometric TUNEL System; Promega Corp., Alexandria, NSW, Australia) was performed on fixed-frozen sections as per manufacturer’s instructions. .. Briefly, sections were washed in PBS, immersed in 0.2% Triton-X in PBS for 20 min and incubated in equilibration buffer (Promega, Alexandria, NSW, Australia) for 10 min prior to the rTdT reaction at 37°C for 1 h in the dark. .. Subsequently, sections were washed 15 min in 2× SSC and PBS (3 × 5 min) before a counter-stain with 1 μg/ml DAPI (Thermo Fisher Scientific) in PBS for 20 min.



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