epps buffer (Merck & Co)

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Merck & Co epps buffer
Epps Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/epps+buffer/buffer/pm41395939-320-22-24
Average 86 stars, based on 1 article reviews

epps buffer - by Bioz Stars, 2026-09

86/100 stars

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Sonication:

Article Title: Transient cytoskeletal anisotropy encodes short-term mechanical memory
Article Snippet: .. Cells were lysed in LDS sample buffer (Merck) supplemented with 100 mM DTT, heated at 95°C for 5 min, and sonicated. .. Proteins were separated on 4–12% Bis–Tris gels (Merck) and transferred onto PVDF membranes (Merck).

Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
Article Snippet: Following the treatment, the whole-cell lysates were prepared in ice-cold RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with phenylmethylsulfonyl fluoride (1 mM PMSF, Merck Life Science (Poznań, Poland)), Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific), and Halt Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). .. Then, the samples were sonicated and centrifuged, and mPAGE 4X LDS Sample Buffer supplemented with 50 mM β-mercaptoethanol (Merck Life Science (Poznań, Poland)) was used as a sample buffer. .. A total of 30 μg of proteins was loaded into each lane of the mPAGE Bis-Tris gels, and then, the proteins were separated by SDS polyacrylamide gel electrophoresis at 180 V for 30 min in an electrophoresis tank (Mini-PROTEAN Tetra Cell, Bio-Rad, Hercules, CA, USA) using MOPS SDS running buffer, Merck Life Science (Poznań, Poland).

Blocking Assay:

Article Title: FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN)
Article Snippet: Proteins were transferred to a nitrocellulose membrane (BioRad Trans-Blot Turbo 0.2μm Nitrocellulose, #1704158 and 1704159) using a semi-dry transfer system (BioRad Trans-Blot Turbo Transfer System). .. After transfer, membranes were blocked in blocking buffer; 5% non-fat dry milk (Merck, #70166) in Tris-buffered saline (Holland Moran, #J60877.1) with 0.1% Tween-20 (Sigma-Aldrich, #P6585)(TBST) for 1 hour at room temperature to prevent nonspecific binding. ..

Article Title: A One‐Step Workflow for Size‐Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection
Article Snippet: Proteins were separated by SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to nitrocellulose membranes (cat no. 162‐0115, Bio‐Rad). .. Membranes were blocked for 30 min in blocking buffer (5% non‐fat milk in PBS with 0.5% Tween‐20 (cat no. P1379, Merck Life Science) or 5% bovine serum albumin (BSA) (cat no. 10735108001, Roche) in PBS with 0.5% Tween‐20 for CD9) and incubated with primary antibodies overnight at 4°C. ..

Saline:

Binding Assay:

Incubation:

Article Title: Expression, Solubilization, and Refolding Recovery of a Novel l ‑Asparaginase–Arginase 1 Chimera from E. coli Inclusion Bodies
Article Snippet: .. For dilution, 125 μL protein was added to 2.5 mL buffer at 0.1 mL min –1 , incubated 1 h at 25 °C, centrifuged (16,000× g , 20 min), filtered (0.45 μm), concentrated, and desalted by ultrafiltration (Amicon Ultra 10 kDa, Merck). ..

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