lentiviral vectors plko.1 encoding scrambled shctrl (Addgene inc)
Addgene inc is a verified supplier 
90
Structured Review
Addgene inc
lentiviral vectors plko.1 encoding scrambled shctrl
Lentiviral Vectors Plko.1 Encoding Scrambled Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/encoding+scrambled+shctrl/plko+1/pmc08364641-46-7-10
Average 90 stars, based on 1 article reviews
Lentiviral Vectors Plko.1 Encoding Scrambled Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/encoding+scrambled+shctrl/plko+1/pmc08364641-46-7-10
Average 90 stars, based on 1 article reviews
lentiviral vectors plko.1 encoding scrambled shctrl - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells"
Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells
Journal: Journal of Cancer
doi: 10.7150/jca.45262
Figure Legend Snippet: H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.
Techniques Used: Expressing, Control, Western Blot, Marker
Figure Legend Snippet: ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1 . Apoptosis assay of 2008 ( A ) and SKOV3 ( B ) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). ** P <0.001 compared with the shctrl group. Mean ± SD, n = 3.
Techniques Used: Apoptosis Assay, Expressing
Figure Legend Snippet: Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. ( A ) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H 2 O 2 . ( B and C ) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 ( B ) and SKOV3 ( C ) cells after treatment with 0.2 mM H 2 O 2 .
Techniques Used: Expressing, Western Blot
Related Articles
Synthesized:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Clone Assay:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Knockdown:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Construct:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Next-Generation Sequencing:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Plasmid Preparation:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Cloning:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Incubation:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Generated:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of Sequencing:Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Article Title: Metastatic breast cancer cells adapt to a soft environment with limited involvement of mechanotransduction. Article Snippet: The extracellular matrix (ECM) plays a crucial role in regulating intracellular signaling in tumor cells.. Although the influence of ECM stiffness on tumor malignancy has been well studied, the adaptation of tumors to a soft extratumoral environment with an increasing proliferation rate remains poorly understood.. In this study, we investigated the mechanism of tumor cell adaptation to a soft environment and its relationship with tumor Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease. Article Snippet: Acute pancreatitis was induced by the administration of 7 h intraperitoneal injections of cerulein (HY-A0190; 100 ng/g bodyweight in 0.9% saline; MedChemExpress) after a fasting period of 12 h (water ad libitum). Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: All cell lines were cultured in DMEM high glucose (D6546; Sigma-Aldrich) supplemented with 10% FBS (S1810; Biowest), 10 mM L-glutamine (G7513; Sigma-Aldrich), and 50 U penicillin–streptomycin (15070063; Thermo Fisher Scientific) at 37°C with 5% CO 2 . Article Title: New insights into the mechanism of T-2 toxin cytotoxicity: stress granules inhibit the mTORC2/AKT signaling pathway Article Snippet: T-2 toxin is a highly poisonous mycotoxin, which contaminates crops and feed, causes poisoning of livestock and poultry, and even threatens human health through food residues.. However, there is a lack of specific detoxification agents for T-2 toxin.. Our previous study has shown that stress granules (SGs) most likely contribute significantly to the cytotoxicity of T-2 toxin, but the mechanism remains unclear. Article Title: Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Article Snippet: Glioblastoma (GBM) is an aggressive brain tumor driven by glioma stem cells (GSCs), which contribute to tumor growth and therapeutic resistance.. This study investigates the effects of Gsk3β inhibition on GSC viability, focusing on the role of the canonical WNT signaling pathway.. We found that Gsk3β inhibition activates the WNT pathway, leading to upregulation of Wwc1, which downregulates Yap via Lats1 phosphorylation. Article Title: Constitutive expression of the transcriptional co-activator IκBζ promotes melanoma growth and immunotherapy resistance Article Snippet: For the generation of knockdown or overexpressing melanoma cells, the following constructs were used: Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease Article Snippet: For this, 6 μg of pLKO.1 plasmid, 5.4 μg of |
