Review



empty vector ev control  (Vector Biolabs)


Bioz Verified Symbol Vector Biolabs is a verified supplier
Bioz Manufacturer Symbol Vector Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 16 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis."

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    Journal: Arthritis research & therapy

    doi: 10.1186/s13075-017-1388-8

    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Figure Legend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Techniques Used: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay

    Related Articles

    Knock-Out:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    In Vitro:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Control:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Expressing:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Marker:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Plasmid Preparation:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Construct:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Immunoprecipitation:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Western Blot:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Virus:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Recombinant:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Binding Assay:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Over Expression:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Infection:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..



    Similar Products

    90
    Vigene Biosciences empty vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/pmc10834511-52-6-13
    Average 90 stars, based on 1 article reviews
    empty vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Genecopoeia empty preceiver-lv105 vector (ev) as a control
    Empty Preceiver Lv105 Vector (Ev) As A Control, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/lv105/pmc10351194-42-19-29
    Average 90 stars, based on 1 article reviews
    empty preceiver-lv105 vector (ev) as a control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Sino Biological empty vector ev
    Empty Vector Ev, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCMV3-N-FLAG+Negative+Control+Vector/bio_rxiv__2023__07__10__547766-45-2-13
    Average 93 stars, based on 1 article reviews
    empty vector ev - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Vigene Biosciences empty-vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/ppr0660169-57-5-11
    Average 90 stars, based on 1 article reviews
    empty-vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Genecopoeia control empty vector (ev
    Control Empty Vector (Ev, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vect+control/pmc11072547-195-15-22
    Average 90 stars, based on 1 article reviews
    control empty vector (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    80
    OriGene empty vector ev controls
    Empty Vector Ev Controls, supplied by OriGene, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCas-Guide-Puro-CRISPRa+gene+activation+vector/pm34449924-51-9-16
    Average 80 stars, based on 1 article reviews
    empty vector ev controls - by Bioz Stars, 2026-10
    80/100 stars
      Buy from Supplier

    90
    Millipore p3xflag-cmv empty vector (ev) control
    P3xflag Cmv Empty Vector (Ev) Control, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/p3xflag+cmv+10/pm29208611-90-10-17
    Average 90 stars, based on 1 article reviews
    p3xflag-cmv empty vector (ev) control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)
    Pcdna™6.2/C‐Emgfp‐Gw/Topo™ Only (Empty Vector [Ev], Control), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pmc06590123-74-18-10
    Average 90 stars, based on 1 article reviews
    pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 1 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Journal: Arthritis research & therapy

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    doi: 10.1186/s13075-017-1388-8

    Figure Lengend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Article Snippet: Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs.

    Techniques: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay