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nitrocellulose membranes  (GE Healthcare)


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    Structured Review

    GE Healthcare nitrocellulose membranes
    Nitrocellulose Membranes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+buffer/Amersham+Protran+0%2E45+NC+300mm%C3%974m+1+roll%2FPK/pmc08084160-87-17-20
    Average 96 stars, based on 255 article reviews
    nitrocellulose membranes - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: Suboptimal Mitochondrial Activity Facilitates Nuclear Heat Shock Responses for Proteostasis and Genome Stability
    Article Snippet: .. Protein samples were resolved by SDS-PAGE and transferred to the NC membrane (10600002; GE Healthcare, USA). .. Protein samples were resolved by SDS-PAGE and transferred to the NC membrane (10600002; GE Healthcare, USA).

    Membrane:

    Article Title: Suboptimal Mitochondrial Activity Facilitates Nuclear Heat Shock Responses for Proteostasis and Genome Stability
    Article Snippet: .. Protein samples were resolved by SDS-PAGE and transferred to the NC membrane (10600002; GE Healthcare, USA). .. Protein samples were resolved by SDS-PAGE and transferred to the NC membrane (10600002; GE Healthcare, USA).

    Article Title: Thyrotropin releasing hormone and its cyclised C-terminal peptide inhibit thyroglobulin release from thyroid follicular cells: evidence for acute regulation of hormone production at the thyroid gland
    Article Snippet: .. Wet transfer from gel to Nitrocellulose blotting membrane (10600002, GE Health Care) was set at 20 volts for 20 hr. .. Membranes were blocked with 5% semi-skim milk in PBS-Triton X100 (0.05%) for 1 hr and incubated with primary antibodies 1:104 thyroglobulin (156008, Abcam) produced in rabbit and 1:10000 Monoclonal Anti-BSA antibody produced in mouse (B2901, Sigma Aldrich).

    Article Title: An Imbalance in the Pro/mature BDNF Ratio Occurs in Multiple Brain Regions During Normal Ageing in Wild-Type Mice
    Article Snippet: Stacking gel (10 mL stacking acrylamide, pH 6.8 + 7.5 μL TEMED and 150 μL 10% APS) was then poured on top and brain samples loaded (25 μg of protein) along with a molecular weight marker (BIORAD, AUS, cat#1610374). .. The separation was run at 120 V for 2 h. The gel was then pre-soaked in used 1x transfer buffer along with sponges and filter paper for 20 min. A 0.45 μm nitrocellulose membrane (Amersham, GE healthcare, cat#10600002) was then cut to size and soaked for several minutes. ..

    Article Title: Modulating the Kynurenine pathway or sequestering toxic 3-hydroxykynurenine protects the retina from light-induced damage in Drosophila
    Article Snippet: .. After dilution with RIPA buffer (150 mM sodium chloride, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris pH 8), lysates were heated at 37°C for 30 min. Lysates equivalent to 2.5 heads were loaded and run on two parallel (15% acrylamide) gels, and proteins were transferred onto a membrane (Nitrocellulose Blotting Membrane 10600002; GE Healthcare Life Sciences; PA; USA). .. Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000; MA1-16616, AB_568420, clone P3UI, Thermo Fisher Scientific) and mouse anti-β-Tubulin E7 (1:1000; [ http://dshb.biology.uiowa.edu/tubulin-beta-_2 ] deposited by M. McCutcheon/ S. Carroll to the from Developmental Studies Hybridoma Bank (DSHB), University of Iowa, USA).

    Western Blot:

    Article Title: Expression Analysis of Lipocalin 2 (LCN2) in Reproductive and Non-Reproductive Tissues of Esr1 -Deficient Mice
    Article Snippet: .. Proteins were transferred to Protran ® Western blotting membranes (#10600002, 0.45 μm, GE Healthcare, Buckinghamshire, UK) in NuPAGE buffer containing 10% methanol. ..

    Article Title: Endogenous myoglobin expression in mouse models of mammary carcinoma reduces hypoxia and metastasis in PyMT mice
    Article Snippet: After loading the protein samples at equal concentrations, the gel was run at 25 mA per gel provided by a BioRad Power Pac power supply. .. The proteins were then transferred to a nitrocellulose blotting membrane (GE Healthcare Life Sciences, 10600002) using a Western Blot Cooled Transfer Unit at 1000 V. Membranes were blocked in blocking buffer (5% non-fat dry milk dissolved in TBST) for 1 h at room temperature and then incubated with the primary antibodies diluted in blocking buffer at 4 °C overnight (Rabbit-anti-Myoglobin (FL-154): sc-25607, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti-Cyclin D1: sc-8396, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti- β-actin: A5441, Sigma Aldrich, dilution 1:5000). .. After washing in TBST buffer, the horseradish peroxidase (HRP) conjugated secondary antibodies were diluted 1:5000 in blocking buffer (donkey-anti-rabbit, NA934V, Amersham/ goat-anti-mouse, sc-2031, Santa Cruz Biotechnology) and added to the membranes, which were placed on a shaker for 1 h. Super Signal West Femto Maximum Sensitivity Substrate (ThermoFisher, 34095) was used to detect of the bands in a luminescent image analyzer LAS-3000 (Fujifilm).

    Saline:

    Article Title: The Cytoplasmic Region of SARAF Reduces Triple-Negative Breast Cancer Metastasis through the Regulation of Store-Operated Calcium Entry
    Article Snippet: The lysates were centrifuged at 12,000× g at 4 °C for 10 min. Reducing Sample Buffer (RSB) [62.5 mM Tris-HCl, 2% w / v SDS (Sigma-Aldrich, San Louis, MO, USA, Cat#L5750), 10% v / v glycerol (Merck, Darmstadt, Germany, Cat#356352), 1% v / v β-Mercaptoethanol (Merck, Darmstadt, Germany, Cat #8057400250)] was added to the supernatant samples, and then boiled for 5 min, followed by size fractionation on SDS–PAGE. .. Following SDS-PAGE, proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Chicago, IL, USA, Cat#10600002), which were then blocked for 1 h with BLOTTO [4% w/v nonfat dry milk/ 0.1% v / v Tween-20 in Tris-buffered saline (TBS: 50 mM Tris-HCl, 150 mM NaCl, adjusted to pH 7.5)] followed by 2 h or overnight incubation with primary antibodies. .. After 3 washes with BLOTTO, the membranes were incubated with the appropriate HRP-conjugated secondary antibody for 1 h. After 3 washes for 10 min each with 0.1% v/v Tween-20/TBS, immunoblots were visualized using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, Waltham, MA, USA, Cat#34080).

    Incubation:

    Article Title: The Cytoplasmic Region of SARAF Reduces Triple-Negative Breast Cancer Metastasis through the Regulation of Store-Operated Calcium Entry
    Article Snippet: The lysates were centrifuged at 12,000× g at 4 °C for 10 min. Reducing Sample Buffer (RSB) [62.5 mM Tris-HCl, 2% w / v SDS (Sigma-Aldrich, San Louis, MO, USA, Cat#L5750), 10% v / v glycerol (Merck, Darmstadt, Germany, Cat#356352), 1% v / v β-Mercaptoethanol (Merck, Darmstadt, Germany, Cat #8057400250)] was added to the supernatant samples, and then boiled for 5 min, followed by size fractionation on SDS–PAGE. .. Following SDS-PAGE, proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Chicago, IL, USA, Cat#10600002), which were then blocked for 1 h with BLOTTO [4% w/v nonfat dry milk/ 0.1% v / v Tween-20 in Tris-buffered saline (TBS: 50 mM Tris-HCl, 150 mM NaCl, adjusted to pH 7.5)] followed by 2 h or overnight incubation with primary antibodies. .. After 3 washes with BLOTTO, the membranes were incubated with the appropriate HRP-conjugated secondary antibody for 1 h. After 3 washes for 10 min each with 0.1% v/v Tween-20/TBS, immunoblots were visualized using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, Waltham, MA, USA, Cat#34080).

    Article Title: Endogenous myoglobin expression in mouse models of mammary carcinoma reduces hypoxia and metastasis in PyMT mice
    Article Snippet: After loading the protein samples at equal concentrations, the gel was run at 25 mA per gel provided by a BioRad Power Pac power supply. .. The proteins were then transferred to a nitrocellulose blotting membrane (GE Healthcare Life Sciences, 10600002) using a Western Blot Cooled Transfer Unit at 1000 V. Membranes were blocked in blocking buffer (5% non-fat dry milk dissolved in TBST) for 1 h at room temperature and then incubated with the primary antibodies diluted in blocking buffer at 4 °C overnight (Rabbit-anti-Myoglobin (FL-154): sc-25607, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti-Cyclin D1: sc-8396, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti- β-actin: A5441, Sigma Aldrich, dilution 1:5000). .. After washing in TBST buffer, the horseradish peroxidase (HRP) conjugated secondary antibodies were diluted 1:5000 in blocking buffer (donkey-anti-rabbit, NA934V, Amersham/ goat-anti-mouse, sc-2031, Santa Cruz Biotechnology) and added to the membranes, which were placed on a shaker for 1 h. Super Signal West Femto Maximum Sensitivity Substrate (ThermoFisher, 34095) was used to detect of the bands in a luminescent image analyzer LAS-3000 (Fujifilm).

    other:

    Article Title: In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells.
    Article Snippet: Protein expression was analyzed using antibodies against talin1/2 (1:500, ab11188, Abcam), kindlin1 (1:1000, ab68041, Abcam), kindlin2 (1:1000, ab74030, Abcam), and vinculin (1:500, ab18058, Abcam).

    Blocking Assay:

    Article Title: Endogenous myoglobin expression in mouse models of mammary carcinoma reduces hypoxia and metastasis in PyMT mice
    Article Snippet: After loading the protein samples at equal concentrations, the gel was run at 25 mA per gel provided by a BioRad Power Pac power supply. .. The proteins were then transferred to a nitrocellulose blotting membrane (GE Healthcare Life Sciences, 10600002) using a Western Blot Cooled Transfer Unit at 1000 V. Membranes were blocked in blocking buffer (5% non-fat dry milk dissolved in TBST) for 1 h at room temperature and then incubated with the primary antibodies diluted in blocking buffer at 4 °C overnight (Rabbit-anti-Myoglobin (FL-154): sc-25607, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti-Cyclin D1: sc-8396, Santa Cruz Biotechnology, dilution 1:200/ mouse-anti- β-actin: A5441, Sigma Aldrich, dilution 1:5000). .. After washing in TBST buffer, the horseradish peroxidase (HRP) conjugated secondary antibodies were diluted 1:5000 in blocking buffer (donkey-anti-rabbit, NA934V, Amersham/ goat-anti-mouse, sc-2031, Santa Cruz Biotechnology) and added to the membranes, which were placed on a shaker for 1 h. Super Signal West Femto Maximum Sensitivity Substrate (ThermoFisher, 34095) was used to detect of the bands in a luminescent image analyzer LAS-3000 (Fujifilm).



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