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acrylamide  (Bio-Rad)


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  • 97

    Structured Review

    Bio-Rad acrylamide
    Acrylamide, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electron+microscope+fixative+buffer/Acrylamide/pmc11298649-404-13-14
    Average 97 stars, based on 10229 article reviews
    acrylamide - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    SDS Page:

    Article Title: Proteomic Profiling and Targeted Validation Reveal a More Extensive Salivary than Serum Response to a Feed-Deprivation Protocol in Horses with Gastric Lesions.
    Article Snippet: .. Samples were separated by SDS-PAGE using a home-made 12% acrylamide mini gel in a vertical electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad, Hercules, CA, USA) at 180 V for 45 min. After separation, proteins were transferred to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) at 180 mV for 20 min. Membranes were then blocked overnight at 4 ◦C in PBS containing 5% non-fat dried milk. ..

    Article Title: Targeting CDC7 Overcomes Gemcitabine Resistance in Cholangiocarcinoma by Inducing Cellular Senescence.
    Article Snippet: 54 Advanced-stage cholangiocarcinoma (CCA) frequently develops resistance to 55 chemotherapy, highlighting an urgent unmet need for novel therapeutic strategies.. Here, 56 we analyzed 16 pairs of CCA and adjacent non-tumor tissues, and demonstrated that 57 dysregulation of the cell cycle machinery is a hallmark of CCA, closely associated with 58 chemoresistance and poor prognosis.. Targeting aberrant cell cycle regulation therefore 59 represents a promising approach to improve current treatment outcomes.

    Electrophoresis:

    Article Title: Proteomic Profiling and Targeted Validation Reveal a More Extensive Salivary than Serum Response to a Feed-Deprivation Protocol in Horses with Gastric Lesions.
    Article Snippet: .. Samples were separated by SDS-PAGE using a home-made 12% acrylamide mini gel in a vertical electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad, Hercules, CA, USA) at 180 V for 45 min. After separation, proteins were transferred to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) at 180 mV for 20 min. Membranes were then blocked overnight at 4 ◦C in PBS containing 5% non-fat dried milk. ..

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Supernatants were extracted and a protein assay was performed using a colorimetric bicinchoninic acid (BCA) protein assay according to manufacturer’s instructions (Thermo Fisher Scientific). .. Diluted samples were loaded into a tris-glycine extended (TGX) FastCast Acrylamide (Bio-Rad) gel and run for 20 min at 60 V, followed by 70 min at 100 V using an electrophoresis cell (Bio-Rad). .. Protein was transferred from the gel onto a polyvinylidene fluoride (PVDF) membrane via a wet transfer for 70 min at 100 V using an electrophoresis cell (Bio-Rad).

    Western Blot:

    Article Title: HMOX1 Is Involved in the Upregulation of Mitochondrial Fatty Acid β‐Oxidation Through Heme Catalysis
    Article Snippet: Intracellular energy metabolism is strictly regulated by hormones and intracellular metabolites for the appropriate use of energy resources such as carbohydrates and fatty acids.. Mitochondria are essential organelles in eukaryotic cells since they contain several metabolic machineries required for oxidative phosphorylation, such as the tricarboxylic cycle, electron transport chain, and fatty acid βoxidation (FAO).. Previously, we reported that FAO activity is increased in CLPXknockout HepG2 cells (CLPXKO cells).

    Protein Concentration:

    Article Title: HMOX1 Is Involved in the Upregulation of Mitochondrial Fatty Acid β‐Oxidation Through Heme Catalysis
    Article Snippet: Intracellular energy metabolism is strictly regulated by hormones and intracellular metabolites for the appropriate use of energy resources such as carbohydrates and fatty acids.. Mitochondria are essential organelles in eukaryotic cells since they contain several metabolic machineries required for oxidative phosphorylation, such as the tricarboxylic cycle, electron transport chain, and fatty acid βoxidation (FAO).. Previously, we reported that FAO activity is increased in CLPXknockout HepG2 cells (CLPXKO cells).

    Incubation:

    Article Title: HMOX1 Is Involved in the Upregulation of Mitochondrial Fatty Acid β‐Oxidation Through Heme Catalysis
    Article Snippet: Intracellular energy metabolism is strictly regulated by hormones and intracellular metabolites for the appropriate use of energy resources such as carbohydrates and fatty acids.. Mitochondria are essential organelles in eukaryotic cells since they contain several metabolic machineries required for oxidative phosphorylation, such as the tricarboxylic cycle, electron transport chain, and fatty acid βoxidation (FAO).. Previously, we reported that FAO activity is increased in CLPXknockout HepG2 cells (CLPXKO cells).

    Acrylamide Gel Assay:

    Article Title: HMOX1 Is Involved in the Upregulation of Mitochondrial Fatty Acid β‐Oxidation Through Heme Catalysis
    Article Snippet: Intracellular energy metabolism is strictly regulated by hormones and intracellular metabolites for the appropriate use of energy resources such as carbohydrates and fatty acids.. Mitochondria are essential organelles in eukaryotic cells since they contain several metabolic machineries required for oxidative phosphorylation, such as the tricarboxylic cycle, electron transport chain, and fatty acid βoxidation (FAO).. Previously, we reported that FAO activity is increased in CLPXknockout HepG2 cells (CLPXKO cells).

    other:

    Article Title: Comparative shelf life of pasteurized camel and bovine milk from a single commercial source: Bacterial community succession, predicted functional potential, and casein integrity.
    Article Snippet: Laemmli buffer, polyacrylamide, Tris-HCl, acrylamide/Bis solution, sodium dodecyl sulfate (SDS), tetramethylethylenediamine (TEMED), ammonium persulfate (APS) and Coomassie dye were purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA).

    Immunoprecipitation:

    Article Title: VASH1 phosphorylation regulates tubulin detyrosination via CNP/PRKG1α signaling in DRG neurons
    Article Snippet: Recombinant proteins were separated on 4–20% gradient polyacrylamide gels (Bio-Rad) and transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad). .. Proteins immunoprecipitated from DRGs were separated on 4–15% gradient acrylamide gels (Bio-Rad) and transferred onto PVDF membranes. ..

    Gel Electrophoresis:

    Article Title: Primary tumor–derived lactate primes prostate cancer cells for a dormant fate
    Article Snippet: .. Samples (20 to 25 ug) were then loaded on 4 to 20% acrylamide precast SDS–polyacrylamide gel electrophoresis gels (#456-8096, Bio-Rad) and then transferred on polyvinylidene difluoride membrane by Trans-Blot Turbo Transfer Pack (#1704157, Bio-Rad). ..

    Membrane:

    Article Title: Primary tumor–derived lactate primes prostate cancer cells for a dormant fate
    Article Snippet: .. Samples (20 to 25 ug) were then loaded on 4 to 20% acrylamide precast SDS–polyacrylamide gel electrophoresis gels (#456-8096, Bio-Rad) and then transferred on polyvinylidene difluoride membrane by Trans-Blot Turbo Transfer Pack (#1704157, Bio-Rad). ..

    Concentration Assay:

    Article Title: Targeting CDC7 Overcomes Gemcitabine Resistance in Cholangiocarcinoma by Inducing Cellular Senescence.
    Article Snippet: 54 Advanced-stage cholangiocarcinoma (CCA) frequently develops resistance to 55 chemotherapy, highlighting an urgent unmet need for novel therapeutic strategies.. Here, 56 we analyzed 16 pairs of CCA and adjacent non-tumor tissues, and demonstrated that 57 dysregulation of the cell cycle machinery is a hallmark of CCA, closely associated with 58 chemoresistance and poor prognosis.. Targeting aberrant cell cycle regulation therefore 59 represents a promising approach to improve current treatment outcomes.



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