5 alpha f iq competent e coli (New England Biolabs)

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New England Biolabs 5 alpha f iq competent e coli

5 Alpha F Iq Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/efficient+navigation+microscope+software/NEB+5-alpha+F+Iq+Competent+E%2E+coli/pmc10879702-38-1-0
Average 96 stars, based on 398 article reviews

5 alpha f iq competent e coli - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Host ZCCHC3 blocks HIV-1 infection and production through a dual mechanism"

Article Title: Host ZCCHC3 blocks HIV-1 infection and production through a dual mechanism

Journal: iScience

doi: 10.1016/j.isci.2024.109107

Figure Legend Snippet:

Techniques Used: Virus, Plasmid Preparation, Clone Assay, Construct, Luciferase, Recombinant, Modification, Transfection, Western Blot, Protease Inhibitor, Staining, DNA Ligation, Cell Isolation, Activation Assay, Stripping Membranes, Cell Viability Assay, In Vitro, Membrane, Isolation, Software, Laser-Scanning Microscopy, Flow Cytometry, Cytometry, Real-time Polymerase Chain Reaction, Mass Spectrometry

Virus:

Article Title: Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BACH1 (1:1000) Bethyl Cat# A303-058A; RRID: AB_10894145 FBXO22 (1:1000) Proteintech Cat# 13606-1-AP; RRID: AB_2104403 NRF2 (1:1000) Cell Signaling Cat# 12721S; RRID: AB_2715528 KEAP1 (1:10 000) Proteintech Cat# 10503-2-AP; RRID: AB_2132625 HMOX1 (1:1000) Bethyl Cat# A303-662A; RRID: AB_11205464 SKP1 (1:5000) Michele Pagano’s lab N/A CUL1 (1:1000) Thermo Fisher Scientific Cat# 718700; RRID: AB_2534002 HA (1:1000) Sigma-Aldrich Cat# H3663; RRID: AB_262051 FLAG (1:4000) Sigma-Aldrich Cat# F1804; RRID: AB_262044 Vinculin (1:1000) Bethyl Cat# A302-535A; RRID: AB_1999080 NCOR1 (1:1000) Cell Signaling Technologies Cat# 5948S; RRID: AB_10834809 MAFF (1:1000) Proteintech Cat# 12771-1-AP; RRID: AB_2137677 PCNA (1:1000) Santa Cruz Biotechnologies Cat# sc-56; RRID: AB_628110 Cyclin D1 (1:1000) Thermo Fisher Scientific Cat# MA1-39546; RRID: AB_11000217 GAPDH (1:1000) Cell Signaling Cat# 97166; RRID: AB_2756824 b-actin (1:5000) Sigma-Aldrich Cat# A5441; RRID: AB_476744 Bacterial and virus strains E. coli NEB5a New England Biolabs Cat# C2992H E. coli NEB Stable New England Biolabs Cat# C3040H E. coli BL21 (DE3) New England Biolabs Cat# C2527I E. coli DH5a New England Biolabs Cat# C2987I E. coli DH10Bac Life Technologies Cat# 10361012 Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000150 Cycloheximide Sigma-Aldrich Cat# C7698-1G MG132 Peptides International Cat# IZL-3175v MLN-4924 Active Biochem Cat# A-1139 Spermine NONOate Enzo Life Sciences Cat# ALX-430-013-5005 S-1-propenyl-L-cysteine MedChemExpress Cat# HY-111827 S-Nitrosoglutathione EMD Millipore Cat# 487920 NOR3 Sigma-Aldrich Cat# E2895 Hemin Sigma-Aldrich Cat# 51280-1G Critical commercial assays S-Nitrosylated Protein Detection Kit (Biotin Switch) Cayman Chemical Cat# 10006518 BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Mycostrip, Mycoplasma Detection Kit Invivogen Cat# rep-mys-50 Deposited data FBXO22-BACH1-BTB structure This paper PDB: 8UA3 SCFFBXO22-BACH1-BTB structure This paper PDB: 8UA6 Monomeric FBXL17-BACH1-BTB structure This paper PDB: 8UAH Monomeric SCFFBXL17-BACH1-BTB structure This paper PDB: 8UBT Dimeric SCFFBXL17-BACH1-BTB structure This paper PDB: 8UBU (Continued on next page) e1 Cell 187, 1–17.e1–e10, December 26, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1 HA-BACH1 (F128D) This paper N/A pcDNA3.1 HA-BACH1 BTB (K129*), *: stop codon This paper N/A pcDNA3.1 HA-FBXL17 DCTH (T675*), *: stop codon This paper N/A pFB-GST-FBXO22 This paper N/A pFB-His-SKP1 This paper N/A pACE-His-MBP-FBXL17 This paper N/A pAL-His-BACH1-BTB This paper N/A pET-GST-BACH1-BTB This paper N/A pET-GST-Avi-BACH1-BTB This paper N/A pAL-His-Avi-BACH1-BTB This paper N/A pAL-His-Avi-BACH1-BTB (C34A) This paper N/A pAL-His-Avi-BACH1-BTB (C55A) This paper N/A pAL-His-Avi-BACH1-BTB (C107A) This paper N/A pAL-His-Avi-BACH1-BTB (C109A) This paper N/A pAL-His-Avi-BACH1-BTB (C122A) This paper N/A pAL-His-Avi-BACH1-BTB (C122E) This paper N/A pET-His-Avi-BACH1-BTB This paper N/A pET-His-Avi-BACH1-BTB (S17C/V20I/V32I/S50A/H60W/S61Q) This paper N/A pAL-MBP-BACH1-BTB This paper N/A pAL-MBP-BACH1-BTB (S17C/V20I/V32I/S50A/H60W/S61Q) This paper N/A pET-GST-Avi-BACH2-BTB This paper N/A pET-GST-BACH2-BTB This paper N/A pAL-His-BACH2-BTB This paper N/A pAL-MBP-BACH2-BTB This paper N/A pET-MSB-BACH1-BTB This paper N/A pACE-His-Venus-BACH1-FL This paper N/A pET-MSB-FLAG-BACH1-BTB This paper N/A pET-GST-MAF This paper N/A pAL-His-MBP-BACH1-bZIP This paper N/A pET-GST-NCOR1 This paper N/A pET-GST-GLMN This paper N/A pET-His-RBX1-His-CUL1 This paper N/A Software and algorithms ImageJ Software 2.0 NIH https://imagej.net/software/imagej2/ GraphPad Prism v9.5.1 Graphpad Software https://www.graphpad.com MotionCor2 Zheng et al.72 https://emcore.ucsf.edu/ucsf-software AlphaFold-Multimer in Google ColabFold Mirdita et al.73 https://colab.research.google.com/ github/sokrypton/ColabFold/blob/ main/AlphaFold2.ipynb UCSF Chimera Pettersen et al.74 https://www.cgl.ucsf.edu/chimera/ CryoSPARC Structura Biotechnology Inc. https://cryosparc.com Coot Emsley et al.75 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ PHENIX Adams et al.76 Afonine et al.77 https://phenix-online.org PyMOL Schrödinger https://www.pymol.org HDExaminer v3 Sierra Analytics https://massspec.com/hdexaminer/ HXExpress v2 Guttman et al.78 https://www.hxms.com/HXExpress/ (Continued on next page) ll OPEN ACCESS e3 Cell 187, 1–17.e1–e10, December 26, 2024 Please cite this article in press as: Cao et al., Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress, Cell (2024), https://doi.org/10.1016/j.cell.2024.10.012 Article

Recombinant:

Article Title: Borrelia burgdorferi BB0346 is an Essential, Structurally Variant LolA Homolog that is Primarily Required for Homeostatic Localization of Periplasmic Lipoproteins
Article Snippet: .. Chemically competent NEB ® 5-alpha F’ Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB) agar plates (BD; 244520) and in LB broth (Fisher; BP1426). .. Plasmid DNA was isolated from E. coli clones using a Miniprep kit (Macherey-Nagel; 740588) and verified by Oxford Nanopore Technology sequencing (Plasmidsaurus) or single-pass primer extension sequencing (ACGT, Inc.) with DNA oligonucleotide primers (Integrated DNA Technologies).

Bicinchoninic Acid Protein Assay:

Plasmid Preparation:

Article Title: Bichromatic Splicing Detector Allows Quantification of THRA1 and THRA2 Splicing Isoforms in Single Cells by Fluorescent Live-Cell Imaging
Article Snippet: This region of interest (from now on called minigene) was first introduced into the pGEM ® -T Easy amplification vector (Promega, #A1360) via TA cloning following the manufacturer’s protocol. .. The obtained plasmid was transformed into high-efficiency competent Escherichia coli NEB ® 5-alpha F’I q (New England Biolabs, #C2992H) following the manufacturer’s protocol. .. Positive clones were validated by colony PCR with primers specific for the two ligation sites and by automated Sanger sequencing (BigDye ® Terminator v1.1 protocol, ABI 3500).

Transformation Assay:

other:

Article Title: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles
Article Snippet: NEB® 5-alpha F’ Iq competent E. coli (New England Biolabs) transformed with the plasmids were grown at 37 °C.

Cloning:

Article Title: Programmed RNA editing with an evolved bacterial adenosine deaminase.
Article Snippet: Programmed RNA editing presents an attractive therapeutic strategy for genetic disease.. In this study, we developed bacterial deaminase-enabled recoding of RNA (DECOR), which employs an evolved Escherichia coli transfer RNA adenosine deaminase, TadA8e, to deposit adenosine-to-inosine editing to CRISPR-specified sites in the human transcriptome.. DECOR functions in a variety of cell types, including human lung fibroblasts, and delivers on-target activity similar to ADAR-overexpressing RNA-editing platforms with 88% lower off-target effects.

Amplification:

Article Title: Conserved yet Divergent Smc5/6 Complex Degradation by Mammalian Hepatitis B Virus X Protein
Article Snippet: The cDNA sequences of the X protein derived from 9 viruses of Orthohepadnavirus were previously synthesized with codon optimization to humans (Twist Bioscience, San Francisco, CA, USA) ( Supplementary Table 2) ( ). cDNA fragments encoding X were cloned into a pCAGGS-mNeonGreen vector ( ) predigested with AgeI-HF (New England Biolabs [NEB], Ipswich, MA, USA, Cat# R3552S) and NheI-HF (NEB, Cat# R3131M) using the In-Fusion Snap Assembly Master Mix (TaKaRa, Cat# Z8947N). .. The plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H) and isolated using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). .. The sequences of all the plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega).

Review