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rnase free sirna duplex re suspension buffer  (OriGene)


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    Structured Review

    OriGene rnase free sirna duplex re suspension buffer
    Rnase Free Sirna Duplex Re Suspension Buffer, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duplex+buffer/siRNA+Related+Product/pm41918423-61-9-14
    Average 94 stars, based on 18 article reviews
    rnase free sirna duplex re suspension buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: NF-κB-Dependent Transcriptional Regulation of Piezo1 Mediates Bacterial Clearance on Stiffened Lung Matrix
    Article Snippet: Bone marrow-derived monocytes (BMDMs) were obtained from 8- to 12-week-old C57BL/6 wild-type (WT), MyD88 -/- , Piezo1 fl/fl , and Piezo1 LysMCre mice (IACUC #2624) and differentiated into macrophages using recombinant mouse M-CSF (50 ng/ml; PeproTech) as previously published.

    Article Title: Glucocorticoids regulate lipid mediator networks by reciprocal modulation of 15-lipoxygenase isoforms affecting inflammation resolution
    Article Snippet: Trilencer-27 universal scrambled negative control siRNA (Origene, SR30004) represents the non-target control (non-T).

    Article Title: Immunoregulatory gene GIMAP6 suppresses lethal atherosclerotic vasculopathy and ischemic heart failure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SuperSignal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat# 34076 Restore Western Blot Stripping Buffer Thermo Fisher Scientific Cat# 21063 Cycloheximide GlpBio Cat# GC17198 Recombinant mouse M-CSF GenScript Cat# Z02930 Recombinant Mouse TNF-α (carrier-free) BioLegend Cat# 575202 Matrigel Growth Factor Reduced Basement Membrane Matrix Corning Life Sciences Cat# 356231 Endothelial cell growth supplement Sigma-Aldrich Cat# E2759 Heparin sodium salt Sigma-Aldrich Cat# H3393 Dispase I Sigma-Aldrich Cat# D4818 Fibronectin Sigma-Aldrich Cat# F1141 Sulfosuccinimidyl oleate Cayman Chemical Cat# 11211 HEPES sodium salt Sigma-Aldrich Cat# H7006 ITS Liquid Media Supplement (100×) Sigma-Aldrich Cat# I3146 BODIPY FL C16 Thermo Fisher Scientific Cat# D3821 BODIPY 493/503 GLPbio Technology Inc Cat# GC42959 MitoSOX Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat# M36008 MitoTEMPO MCE Cat# HY-112879 N-acetyl-L-cysteine Millipore Sigma Cat# A9165-5G GW9662 MCE Cat# HY-16578 GW3965 MCE Cat# HY-10627 Oxidized Low-density Lipoprotein (OxLDL) Thermo Fisher Scientific Cat# L34357 Pierce Dimethylsulfoxide (DMSO), Sequencing Grade Thermo Fisher Scientific Cat# 20688 eBioscience Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 Human TruStain FcX BioLegend Cat# 422302 Nuclease-Free Duplex Buffer Integrated DNA Technologies Cat# 11-01-03-01 ATTO550-labeled tracrRNA Integrated DNA Technologies Cat# 1075927 Cas9 protein MacroLab, UC Berkeley N/A RNase-free siRNA duplex resuspension buffer OriGene Cat# SR30005 Non-targeting control siRNA OriGene Cat# SR30004 GIMAP6 siRNA pool OriGene Cat# SR318628 SPLASH Lipidomix Mass Spec Standard Avanti Polar Lipids Cat# 330707 Methanol, LC-MS grade Fisher Scientific Cat# A456 Isopropanol, LC-MS grade Fisher Scientific Cat# A461 Acetonitrile, LC-MS grade Fisher Scientific Cat# A955 Water, LC-MS grade Fisher Scientific Cat# W6212 Chloroform, LC-MS grade Sigma-Aldrich Cat# 650471-1L Methyl tert-butyl ether Honeywell Cat# 34875-1L Butylated hydroxytoluene Sigma-Aldrich Cat# B1253-500G Ammonium formate Thermo Fisher Scientific Cat# 014517.30 Ammonium carbonate Thermo Fisher Scientific Cat# 196655000 Formic acid, LC-MS grade Thermo Fisher Scientific Cat# 28905 Medronic acid MCE Cat# HY-108309 Sodium Pyruvate Solution MCE Cat# HY-K3024 D-glucose Sigma-Aldrich Cat# G8270 (Continued on next page) e3 Cell 189, 6792–6809.e1–e21, October 15, 2026

    Incubation:

    Article Title: A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart
    Article Snippet: Then, Fermt2 Mouse siRNA Oligo duplexes (Origene #SR419055) were combined with Lipofectamine RNAimax in OptiMEM Reduced Serum medium (ThermoFisher #51985034) for 5 min at room temperature (final concentration 10 nM). .. These complexes were added directly into the culture growth medium, and incubation was performed for 72 h. Negative control cells were transfected with 10 nM of Trilencer-27 Universal Scrambled Negative Control siRNA Duplex (Origene catSR30005). .. To ensure Kindlin-2 overexpression, old cardiac fibroblasts were transfected with 2 μg of Fermt2 Mouse Untagged clone plasmid (Origene #MC202155) by using FuGene transfection reagent.

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Negative Control:

    Article Title: A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart
    Article Snippet: Then, Fermt2 Mouse siRNA Oligo duplexes (Origene #SR419055) were combined with Lipofectamine RNAimax in OptiMEM Reduced Serum medium (ThermoFisher #51985034) for 5 min at room temperature (final concentration 10 nM). .. These complexes were added directly into the culture growth medium, and incubation was performed for 72 h. Negative control cells were transfected with 10 nM of Trilencer-27 Universal Scrambled Negative Control siRNA Duplex (Origene catSR30005). .. To ensure Kindlin-2 overexpression, old cardiac fibroblasts were transfected with 2 μg of Fermt2 Mouse Untagged clone plasmid (Origene #MC202155) by using FuGene transfection reagent.

    Article Title: A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart
    Article Snippet: Fermt2 Mouse siRNA Oligo duplexes , Origene , SR419055. .. Trilencer-27 Universal Scrambled Negative Control siRNA Duplex , Origene , SR30005. .. Fermt2 Mouse Untagged clone plasmid , Origene , MC202155.

    Transfection:

    Article Title: A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart
    Article Snippet: Then, Fermt2 Mouse siRNA Oligo duplexes (Origene #SR419055) were combined with Lipofectamine RNAimax in OptiMEM Reduced Serum medium (ThermoFisher #51985034) for 5 min at room temperature (final concentration 10 nM). .. These complexes were added directly into the culture growth medium, and incubation was performed for 72 h. Negative control cells were transfected with 10 nM of Trilencer-27 Universal Scrambled Negative Control siRNA Duplex (Origene catSR30005). .. To ensure Kindlin-2 overexpression, old cardiac fibroblasts were transfected with 2 μg of Fermt2 Mouse Untagged clone plasmid (Origene #MC202155) by using FuGene transfection reagent.

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Article Title: Targeting the proliferation of glioblastoma cells and enhancement of doxorubicin and temozolomide cytotoxicity through inhibition of PFKFB4 and HMOX1 genes with siRNAs.
    Article Snippet: Transfections were performed using Lipofectamine RNAiMAX (Invitrogen) in serum-free media, following the recommended protocol. .. A scrambled Scientific Reports | (2025) 15:27861 2| https://doi.org/10.1038/s41598-025-97192-z siRNA (OriGene) served as a control to verify transfection efficiency and minimize off-target effects. ..

    Cell Culture:

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Recombinant:

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Knockdown:

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.
    Article Snippet: The antibodies used were as follows: CSA (Abcam, ab137033), CSB (Abcam, ab96089), QPRT (Biorybt, orb317756), ATF3 (Abcam, ab207434), β Actin (SantaCruz, sc47778), GAPDH (Cell Signallng Technology, 2118), Havcr-1 (ThermoFischer scientific, PA5-98302). .. HK-2 (Human kidney proximal tubular epithelial cell line (ATCC CRL-2190)) cells were cultured in Keratinocyte serum-free medium (K-SFM) containing bovine pituitary extract (BPE), human recombinant epidermal growth factor (EGF) in a humidified chamber under 5% CO2 at 37 °C. siRNA knockdown siRNAs for CSA (SR319723A; SR319723C) and CSB (SR320072A; SR320072B) and non-targeted siRNA (SR3005) were purchased from Origene (Rockville, MD, USA). siRNA was diluted with K-SFM to a final concentration of 50 nM, mixed with JetPrime (Polyplus transfection, Illkirch-graffenstaden, France), incubated for 15min at room temperature, and transfected into target cells according to the manufacturer’s instructions. ..

    Control:

    Article Title: Targeting the proliferation of glioblastoma cells and enhancement of doxorubicin and temozolomide cytotoxicity through inhibition of PFKFB4 and HMOX1 genes with siRNAs.
    Article Snippet: Transfections were performed using Lipofectamine RNAiMAX (Invitrogen) in serum-free media, following the recommended protocol. .. A scrambled Scientific Reports | (2025) 15:27861 2| https://doi.org/10.1038/s41598-025-97192-z siRNA (OriGene) served as a control to verify transfection efficiency and minimize off-target effects. ..

    Article Title: Overexpression of the erythropoietin receptor is associated with upregulation of tubulin beta 6 and paclitaxel resistance in ovarian adenocarcinoma cells.
    Article Snippet: .. Both the target and control siRNAs were dissolved in RNase-free siRNA duplex re-suspension buffer (OriGene Technologies, Inc.; #SR30005) according to the manufacturer’s instructions. ..



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