dot blot cytokine expression analysis dot blot cytokine expression arrays  (R&D Systems)

Journal: Biomaterials science

Article Title: Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models.

doi: 10.1039/d4bm01569j

Figure Lengend Snippet: Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

Article Snippet: Cytokine profiling of the EV groups was performed using the dot blot-based Proteome Profiler Human XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions.

Techniques:

Journal: Molecular cancer therapeutics

Article Title: Tetrandrine (TET) Induces Death Receptors: Apo Trail R1 (DR4) and Apo Trail R2 (DR5) and Sensitizes Prostate Cancer Cells to TRAIL Induced Apoptosis

doi: 10.1158/1535-7163.MCT-17-1157

Figure Lengend Snippet: A&B. Western blot analysis showing the effect of TET, TRAIL and combination effect of TET with TRAIL on cleaved caspases (caspase-8 and 3) in scrl (Fig. 4A) and DK cells (Fig. 4B). Different concentrations of TET (10–20µM) and TRAIL (80ng/ml) and combinations of TET and TRAIL were used for 24h and 48h. C&D. Apoptosis assay showing the effect of TET, TRAIL and combination treatment of TET and TRAIL on cell death in LNCaP scrl (Fig. 4C) and DK cells (Fig. 4D) at 48h. E. Representative graph showing the percent live and apoptotic scrl and DK cells after TET, TRAIL and combination treatment of TET and TRAIL at 48h. Averages are representative of 3 independent experiments. Error bars represent standard deviation. *p value <0.05; **p value <0.01; ***p value <0.001, ****p value <0.0001.

Article Snippet: Dot Blot Array Proteome ProfilerTM Human Apoptosis Array Kit, catalog no. ARY009, (R&D systems, Minneapolis, MN) was used to detect apoptosis-related proteins.

Techniques: Western Blot, Apoptosis Assay, Standard Deviation

Journal: Molecular cancer therapeutics

Article Title: Tetrandrine (TET) Induces Death Receptors: Apo Trail R1 (DR4) and Apo Trail R2 (DR5) and Sensitizes Prostate Cancer Cells to TRAIL Induced Apoptosis

doi: 10.1158/1535-7163.MCT-17-1157

Figure Lengend Snippet: PCa cells survive because of absence of death receptors hence no apoptosis takes place. These cells develop resistance to TRAIL therefore TRAIL is unable to induce cell death. Upon TET treatment, there is an increase in DR4 and DR5 mRNA and protein levels which cause apoptosis and further treatment of PCa cells with TRAIL sensitizes these cells to TRAIL which enhances activation of caspases and massive cell death.

Article Snippet: Dot Blot Array Proteome ProfilerTM Human Apoptosis Array Kit, catalog no. ARY009, (R&D systems, Minneapolis, MN) was used to detect apoptosis-related proteins.

Techniques: Activation Assay