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lasergene software  (DNASTAR)


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    Structured Review

    DNASTAR lasergene software
    Lasergene Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnastar+s+editseq+program/MegAlign/pm22008244-188-21-26
    Average 99 stars, based on 5970 article reviews
    lasergene software - by Bioz Stars, 2026-11
    99/100 stars

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    Software:

    Article Title: Development and Application of an eDNA-Based MIRA-LFD Assay for the Detection of the Chinhai Spiny Newt
    Article Snippet: .. Homology alignment was conducted using the MegAlign software package (Lasergene v7.1, DNASTAR Inc., Madison, WI, USA). ..

    Article Title: Pathological injury and gastrointestinal microbiota changes in lambs infected with an Eimeria ovinoidalis isolate from Gansu, China.
    Article Snippet: Coccidiosis poses a serious threat to the sheep industry, with Eimeria ovinoidalis recognized as the primary causative agent.. However, quantitative analyses of the pathological damage induced by Eimeria ovinoidalis infection across the entire intestinal tract of sheep, together with investigations into the structural characteristics of the gastrointestinal microbiota, remain scarce.. In this study, Eimeria ovinoidalis was isolated and purified from the feces of naturally infected sheep in Gansu Province, China.

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: PAM-free dsDNA substrates enable Cas12a trans-cleavage activation for sensitive detection of Vibrio harveyi
    Article Snippet: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas12a-based diagnostics rely on the presence of a protospacer adjacent motif (PAM) to initiate trans-cleavage activity, limiting their utility for diverse nucleic acid targets.. Here, we report a PAM-free Cas12a detection (PFCD) platform that enables robust activation of Cas12a using double-stranded DNA (dsDNA) substrates without canonical PAM sequences.. Through systematic evaluation of target positioning, fragment length, and crRNA spacer composition, we establish that dsDNA fragments bearing guide-adjacent target sites with optimized G/C content can efficiently activate Cas12a.

    Sequencing:

    Article Title: Pathological injury and gastrointestinal microbiota changes in lambs infected with an Eimeria ovinoidalis isolate from Gansu, China.
    Article Snippet: Coccidiosis poses a serious threat to the sheep industry, with Eimeria ovinoidalis recognized as the primary causative agent.. However, quantitative analyses of the pathological damage induced by Eimeria ovinoidalis infection across the entire intestinal tract of sheep, together with investigations into the structural characteristics of the gastrointestinal microbiota, remain scarce.. In this study, Eimeria ovinoidalis was isolated and purified from the feces of naturally infected sheep in Gansu Province, China.

    Article Title: Human febrile illness associated with Yanggou tick virus: Clinical characteristics and epidemiological links to rodents.
    Article Snippet: .. Sequence identities were assessed using the MegAlign tool from the DNAstar package. ..

    Article Title: Immunogenicity of recombinant porcine parainfluenza virus 5 expressing the spike protein of porcine epidemic diarrhea virus.
    Article Snippet: The porcine epidemic diarrhea virus (PEDV) induces severe diarrhea in neonatal piglets, often resulting in high mortality rates.. Consequently, the development of effective and safe vaccines is a critical priority for controlling PEDV infection.. Vaccines vectored by the parainfluenza virus 5 (PIV5) exhibit distinct advantages in terms of safety, genomic stability, and immunogenicity.

    Article Title: PAM-free dsDNA substrates enable Cas12a trans-cleavage activation for sensitive detection of Vibrio harveyi
    Article Snippet: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas12a-based diagnostics rely on the presence of a protospacer adjacent motif (PAM) to initiate trans-cleavage activity, limiting their utility for diverse nucleic acid targets.. Here, we report a PAM-free Cas12a detection (PFCD) platform that enables robust activation of Cas12a using double-stranded DNA (dsDNA) substrates without canonical PAM sequences.. Through systematic evaluation of target positioning, fragment length, and crRNA spacer composition, we establish that dsDNA fragments bearing guide-adjacent target sites with optimized G/C content can efficiently activate Cas12a.

    Article Title: Isolation, identification, and pathogenicity comparison of three NADC30-like PRRSV variants with distinct mutational patterns.
    Article Snippet: .. Complete genome and ORF5 nucleotide sequences were aligned using MegAlign (DNASTAR Inc., USA) to calculate nucleotide sequence iden tities. ..

    Article Title: Isolation, complete genome characterization and phylogenetic analysis of getah virus from a diseased pig herd in Hunan Province, China.
    Article Snippet: Getah virus (GETV) is a mosquito-borne alphavirus (family Togaviridae) that poses a significant threat to the swine industry by causing reproductive disorders in sows and neurological symptoms in piglets.. In this study, a GETV strain, designated HN-2025, was isolated from a commercial pig farm in Hunan Province, China, during a clinical outbreak.. The isolate induced typical cytopathic effects (CPE) in BHK-21 cells, including cell rounding, shrinkage, and detachment.



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    DNASTAR dnastar s editseq program
    Dnastar S Editseq Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnastar+s+editseq+program/EditSeq/pm41934766-144-3-3
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