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bsai restriction endonuclease  (New England Biolabs)


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    New England Biolabs bsai restriction endonuclease

    Bsai Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+sequence+software+program/BsaI-HF+v2/pmc07901297-139-0-4
    Average 97 stars, based on 2179 article reviews
    bsai restriction endonuclease - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "A trans -complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence"

    Article Title: A trans -complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence

    Journal: Cell

    doi: 10.1016/j.cell.2021.02.044


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, Electroporation, Gel Extraction, Electron Microscopy, Synthesized, Sequencing, Software

    Related Articles

    Plasmid Preparation:

    Article Title: The DCBLD2 super-enhancer drives colorectal cancer progression through FOSL2/JUND-mediated activation of the CD146/AKT/TNFRSF6B pathway
    Article Snippet: .. Annealed sgRNAs oligonucleotides were ligated into the PX458 vector following double digestion with Bbs I and Bsa I (NEB). ..

    Article Title: Establishing Yarrowia lipolytica for aloesone biosynthesis through lipid-metabolism rewiring and carbon-source evaluation
    Article Snippet: .. Vector assembly was carried out by Golden Gate cloning using the Y. lipolytica Golden Gate Toolkit (Addgene Kit #1000000167) [33] together with the Type IIS restriction enzyme BsaI-HFv2 and NEBridge Ligase Strains Description Reference XEV PO1f ssXR, ssXDH, ssXK [32] YlKR XEV ΔKU70::ScRAD52 [32] YlALS YlKR ALS This study YlALSDP YlALS ΔDGA1::PEX10 This study Table 1 List of strains used in this study Article in Press Journal of Biological Engineering https://doi.org/10.1186/s13036-026-00776-0 Master Mix (New England Biolabs, Ipswich, MA, USA). .. The resulting pCPT12-RedStar cassette was used as an intermediate construct for target-gene replacement.

    Construct:

    Article Title: Using Polycistronic gRNAs and a tRNA Cleavage System in CRISPR-Based Genome Editing of Toxoplasma gondii
    Article Snippet: .. This was initially digested by BsaI-HFv2 (NEB) to remove the mScarlett construct, after which the fragments were inserted via Gibson assembly (NEB Gibson Assembly Cloning Kit). .. The plasmids constructed were transformed by heat shock and amplified using DH5α chemically competent E. coli (NEB) and extracted using the Monarch Spin Plasmid Miniprep Kit (NEB).

    Article Title: NanoLuciferase Reporter Assays to Investigate Protein Dynamics and Circadian Clock Regulation in the Diatom Model Species Phaeodactylum tricornutum.
    Article Snippet: .. Transcriptional reporter constructs were assembled into L1 level by combining 20 fmol of RITMO1p-AC, RITMO1t-EF, and NLuc-CE containing pL0 vectors and 20 fmol of the pCAO2 receiver vector, with BsaI-HFv2 (NEB) (5u), Ligase (NEB) (100u), during 20 cycles of 37°C for 5 min and 16°C for 5 min. .. Assembly reactions were used to transform NEB5alpha E. coli strain (NEB).

    Cloning:

    Article Title: Using Polycistronic gRNAs and a tRNA Cleavage System in CRISPR-Based Genome Editing of Toxoplasma gondii
    Article Snippet: .. This was initially digested by BsaI-HFv2 (NEB) to remove the mScarlett construct, after which the fragments were inserted via Gibson assembly (NEB Gibson Assembly Cloning Kit). .. The plasmids constructed were transformed by heat shock and amplified using DH5α chemically competent E. coli (NEB) and extracted using the Monarch Spin Plasmid Miniprep Kit (NEB).

    Article Title: Establishing Yarrowia lipolytica for aloesone biosynthesis through lipid-metabolism rewiring and carbon-source evaluation
    Article Snippet: .. Vector assembly was carried out by Golden Gate cloning using the Y. lipolytica Golden Gate Toolkit (Addgene Kit #1000000167) [33] together with the Type IIS restriction enzyme BsaI-HFv2 and NEBridge Ligase Strains Description Reference XEV PO1f ssXR, ssXDH, ssXK [32] YlKR XEV ΔKU70::ScRAD52 [32] YlALS YlKR ALS This study YlALSDP YlALS ΔDGA1::PEX10 This study Table 1 List of strains used in this study Article in Press Journal of Biological Engineering https://doi.org/10.1186/s13036-026-00776-0 Master Mix (New England Biolabs, Ipswich, MA, USA). .. The resulting pCPT12-RedStar cassette was used as an intermediate construct for target-gene replacement.

    Article Title: Design and characterization of peptide-responsive biosensors
    Article Snippet: Genes encoding all genetic parts were synthesized by Twist Bioscience as non-clonal gene fragments with adaptor sequences. .. Plasmids were assembled using Golden Gate cloning by combining DNA fragments with BsaI-HF v2, T7 DNA ligase, and T4 DNA ligase buffer (all New England Biolabs) in reactions. ..

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.

    Polymerase Chain Reaction:

    Article Title: Machine learning-assisted directed evolution of plant Rubisco.
    Article Snippet: .. Fragments were PCR purified (Omega) and assembled using the Golden Gate Assembly via BsaI- HF (New England Biolabs) cutting and T4 ligase (New England Biolabs) ligation overnight. .. The product of the Golden Gate reaction was purified (Omega), 100 ng was electroporated into NEB Stable E. coli (New England Biolabs), and the outgrowth was plated onto 10 LB agar plates containing ampicillin (100 μg/ml).

    Purification:

    Article Title: Machine learning-assisted directed evolution of plant Rubisco.
    Article Snippet: .. Fragments were PCR purified (Omega) and assembled using the Golden Gate Assembly via BsaI- HF (New England Biolabs) cutting and T4 ligase (New England Biolabs) ligation overnight. .. The product of the Golden Gate reaction was purified (Omega), 100 ng was electroporated into NEB Stable E. coli (New England Biolabs), and the outgrowth was plated onto 10 LB agar plates containing ampicillin (100 μg/ml).

    Golden Gate Assembly:

    Article Title: Machine learning-assisted directed evolution of plant Rubisco.
    Article Snippet: .. Fragments were PCR purified (Omega) and assembled using the Golden Gate Assembly via BsaI- HF (New England Biolabs) cutting and T4 ligase (New England Biolabs) ligation overnight. .. The product of the Golden Gate reaction was purified (Omega), 100 ng was electroporated into NEB Stable E. coli (New England Biolabs), and the outgrowth was plated onto 10 LB agar plates containing ampicillin (100 μg/ml).

    Ligation:

    Article Title: Machine learning-assisted directed evolution of plant Rubisco.
    Article Snippet: .. Fragments were PCR purified (Omega) and assembled using the Golden Gate Assembly via BsaI- HF (New England Biolabs) cutting and T4 ligase (New England Biolabs) ligation overnight. .. The product of the Golden Gate reaction was purified (Omega), 100 ng was electroporated into NEB Stable E. coli (New England Biolabs), and the outgrowth was plated onto 10 LB agar plates containing ampicillin (100 μg/ml).

    Article Title: PRIME: A modular plasmid-based platform for continuous in vivo evolution across bacteria
    Article Snippet: PCR products were digested with XbaI and NotI restriction enzymes (New England Biolabs), ligated, and transformed into DH10B cells, yielding pORM_CAT_YFP. .. The YFP gene was then replaced with the operon encoding for the orthogonal replication machinery by digestion with BsaI-HF-v2 and KpnI-HF restriction enzymes (New England Biolabs) and ligation with a similarly digested insert, yielding pORM_P12_P19. ..

    Recombinant:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.

    Sequencing:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.

    Real-time Polymerase Chain Reaction:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.

    Modification:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.

    Software:

    Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Phanta Max Super-Fidelity DNA polymerase Vazyme P505 BasI-HFv2 NEB R3733 ClonExpress II One Step Cloning Kit Vazyme C116 DNA Ligase Solution I Takara 6022Q Dulbecco’s modified Eagle’s medium Gibco 12491015 FBS Gemini 100-106 Penicillin–streptomycin Gibco 15140-122 jetPRIME Polyplus 101000046 E. coli cells Sangon B528413 Software FlowJo v10 https://www.flowjo.com/flowjo10/ download Reagent/resource Reference or source Identifier or catalog number MEGA 12 https://www.megasoftware.net/ CRISPResso2 https://crispresso.pinellolab.org/ submission Cas-OFFinder http://www.rgenome.net/casoffinder ImageJ https://imagej.nih.gov/ij/index.html GraphPad Prism 8 https://www.graphpad.com Snapgene https://www.snapgene.com/ PyMol https://pymol.org/ Other Illumina NovaSeq (PE150) Novogene Technology (Beijing, China) Annaroad Gene Technology (Beijing, China) .. Mammalian codon-optimized sequences of Cas12n derived from Actinomadura craniellae (Ac) and Corynebacterium glutamicum (Cg) were synthesized by Genewiz and cloned into a CMV promoter-driven eukaryotic expression vector.



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    dna sequence software program  (MacVector inc)
    90
    MacVector inc dna sequence software program
    Dna Sequence Software Program, supplied by MacVector inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    dna sequence software program - by Bioz Stars, 2026-11
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    prism dna sequence analysis software program  (Thermo Fisher)
    98
    Thermo Fisher prism dna sequence analysis software program
    Prism Dna Sequence Analysis Software Program, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prism dna sequence analysis software program - by Bioz Stars, 2026-11
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