assays nebnext ultra iitm dna library prep kit new england biolabs (New England Biolabs)

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New England Biolabs assays nebnext ultra iitm dna library prep kit new england biolabs
Assays Nebnext Ultra Iitm Dna Library Prep Kit New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequence+software+program/NEBNext+Ultra+II+FS+DNA+Library+Prep+with+Sample+Purification+Beads/pm33338432-349-10-11
Average 95 stars, based on 894 article reviews

assays nebnext ultra iitm dna library prep kit new england biolabs - by Bioz Stars, 2026-10

95/100 stars

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Sequencing:

Article Title: High-throughput screening reveals mechanisms of environmental control of germination in a fungal thermophile.
Article Snippet: .. We prepared T-DNA genome junction sequencing libraries following the general TnSeq workflow of reference 2, but using an enzymatic-fragmentation workflow based on the NEBNext Ultra II FS DNA Library Prep Kit with Sample Purification Beads for Illumina (New England Biolabs #E6177S). ..

Article Title: A gap-free, telomere-to-telomere genome assembly for the Caenorhabditis briggsae reference strain AF16
Article Snippet: DNA concentration was determined using the Qubit dsDNA Broad Range Assay kit (Invitrogen, Catalog #Q32853). .. Sequencing libraries were generated using NEBNext® UltraTM II FS DNA Library Prep (New England Biolabs, Catalog #E6177L). .. Samples were sent for sequencing at the Novogene facility, Sacramento, California.

Enzymatic Fragmentation:

Sample Purification:

Article Title: Tissue-specific DNA repair strategies underlie apparent high transposon activity in the Caenorhabditis elegans soma
Article Snippet: .. C. elegans genomic DNA was extracted using Gentra Puregene Tissue Kit (Qiagen, #158667), and DNA library preparation was performed using NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads Kit (New England Biolabs, #E6177). .. Whole-genome sequencing was performed using an Illumina NextSeq instrument, with an Illumina NextSeq Mid-Output 300-cycle kit to obtain paired-end 150 bp reads at 30× coverage (Biopolymers Facility, Harvard Medical School).

DNA Library Preparation:

Generated:

Purification:

Article Title: Blubber under stress: ex vivo cortisol exposure induces an anti-inflammatory state in precision-cut adipose tissue slices from humpback whales
Article Snippet: The duplicate isolates were pooled and cleaned according tomanufacturer’s protocol, using the Qiagen lipid mini kit (RNeasy Lipid Tissue Mini Kit, QIAGEN) with on-column DNase treatment (RNase-free DNase Set, QIAGEN). .. The RNA was eluted in 50 μL of nuclease-free water (QIAGEN) and purified with 1X volume of purification beads (NebNext purification beads, New England Biolabs, Ipswich, MA, USA). .. The final volume of 20 μL RNA held an average yield of 62.01 ± 35.57 ng/μL, with the purity of 2.00 ± 0.08 (260/280) and 1.69 ± 0.23 (260/230), measured with spectrophotometer (NanoDrop, Thermo Scientific).

Construct:

Article Title: Global genomic diversity of the selfing nematode Caenorhabditis tropicalis correlates with geography
Article Snippet: The DNA concentration was determined for each sample with the Qubit dsDNA Broad Range Assay Kit (cat# Q32850 Invitrogen, Carlsbad, CA). .. All 785 strains were used to construct short-read libraries using NEBNext® UltraTM II FS DNA Library Prep (cat# E6177L). .. These libraries were sequenced on the Illumina NovaSeq 6000 (paired-end 150 bp reads) in Duke Center for Genomic and Computational Biology, Novogene, NUSeq, and the New York University core facility.

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