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dna microarray analysis  (Hokkaido System Science Co)

 
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    Hokkaido System Science Co dna microarray analysis
    Dna Microarray Analysis, supplied by Hokkaido System Science Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+systems/dna+microarray+analysis/pm19609979-195-34-40
    Average 90 stars, based on 1 article reviews
    dna microarray analysis - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Importance of Proteasome Gene Expression during Model Dough Fermentation after Preservation of Baker's Yeast Cells by Freezing
    Article Snippet: .. The DNA microarray analysis and the 395 whole genome resequencing experiment were carried out by Hokkaido System Science 396 Co., Ltd. 397 We declare no conflicts of interest. ..

    Article Title: Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis.
    Article Snippet: .. We thank Dr. Hiroshi Miyanishi of the University of Miyazaki for his technical support in RNA extraction, and Hokkaido System Science Co., Ltd. for performing DNA microarray analysis. ..

    Article Title: Importance of Proteasome Gene Expression during Model Dough Fermentation after Preservation of Baker's Yeast Cells by Freezing
    Article Snippet: .. The DNA microarray analysis and the whole-genome resequencing experiment were carried out by Hokkaido System Science Co., Ltd. We declare no conflicts of interest. ..

    Article Title: Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry.
    Article Snippet: The amplified-labeled cRNA was 228 hybridized to a 4×44K-1-color eS13 Neochlamydia DNA microarray customized with 229 bacterial annotation information (Agilent Technologies) [11], and then analyzed using a 230 microarray scanner (Agilent Technologies). .. Hokkaido System Science (Sapporo, Japan) 231 carried out the DNA microarray analysis including the customized array and 232 normalization of gene expression. ..

    Article Title: Perinatal exposure to tetrabromobisphenol A (TBBPA), a brominated flame retardant, exacerbated the pneumonia in respiratory syncytial virus (RSV)-infected offspring mice.
    Article Snippet: For a DNA microarray test of the lung tissues, the lungs were removed on day 1 post-infection and immediately placed in RNAlater reagent (Qiagen, Hilden, Germany) and stored at 4°C until use. .. DNA microarray analysis of the lungs of mock- or RSV-infected mice was performed by Hokkaido System Science Co., Ltd. (Hokkaido, Japan). ..

    Article Title: Comparative Effects of Allulose, Fructose, and Glucose on the Small Intestine.
    Article Snippet: .. DNA microarray analysis was consigned to Hokkaido System Science Co., Ltd. (Sapporo, Hokkaido, Japan) SurePrint G3 Rat 8 × 60 K ver.2.0 (Agilent Technologies, Santa Clara, CA, USA) was used for one color microarray analysis. ..

    Article Title: Cadmium Toxicity Is Regulated by Peroxisome Proliferator-Activated Receptor δ in Human Proximal Tubular Cells
    Article Snippet: RNA quantity and purity were measured using a BioSpec-nano spectrophotometer (Shimadzu, Kyoto, Japan). .. DNA microarray analysis was performed by Hokkaido System Science Co., Ltd. (Sapporo, Japan). .. Complementary RNA (cRNA) was synthesized from 50 ng total RNA using a Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA).

    other:

    Article Title: Transcriptome composition of the preoptic area in mid-age and escitalopram treatment in male mice.
    Article Snippet: The decrease in serotonergic neurotransmission during aging can increase the risk of neuropsychiatric diseases such as depression in elderly population and decline the reproductive system.. Therefore, it is important to understand the age-associated molecular mechanisms of brain aging.. In this study, the effect of aging and chronic escitalopram (antidepressant) treatment to admit mice was investigated by comparing transcriptomes in the preoptic area (POA) which is a key nucleus for reproduction.

    RNA Extraction:

    Article Title: Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis.
    Article Snippet: .. We thank Dr. Hiroshi Miyanishi of the University of Miyazaki for his technical support in RNA extraction, and Hokkaido System Science Co., Ltd. for performing DNA microarray analysis. ..

    Gene Expression:

    Article Title: Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry.
    Article Snippet: The amplified-labeled cRNA was 228 hybridized to a 4×44K-1-color eS13 Neochlamydia DNA microarray customized with 229 bacterial annotation information (Agilent Technologies) [11], and then analyzed using a 230 microarray scanner (Agilent Technologies). .. Hokkaido System Science (Sapporo, Japan) 231 carried out the DNA microarray analysis including the customized array and 232 normalization of gene expression. ..



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    Image Search Results


    In this study, genome-wide DNA methylation of 100 samples from 11 patients was assessed. Each patient had multiple primary small intestinal neuroendocrine tumours (ranging from 2-16 per patient), and a subset of 9 patients had a matched normal small intestinal epithelial sample assessed. A subset of 8 patients also had metastases originating from their SI-NETs (ranging from 1-2 per patient). DNA methylation data was used to assess differential methylation relating to the multifocal tumours, the epigenetic clock was used to assess the ‘timing’ of tumour development and metabolic traits predicted by DNA methylation were compared between samples.

    Journal: bioRxiv

    Article Title: Epigenetic investigation of multifocal small intestinal neuroendocrine tumours reveals accelerated ageing of tumours and epigenetic alteration of metabolic genes

    doi: 10.1101/2024.12.02.626017

    Figure Lengend Snippet: In this study, genome-wide DNA methylation of 100 samples from 11 patients was assessed. Each patient had multiple primary small intestinal neuroendocrine tumours (ranging from 2-16 per patient), and a subset of 9 patients had a matched normal small intestinal epithelial sample assessed. A subset of 8 patients also had metastases originating from their SI-NETs (ranging from 1-2 per patient). DNA methylation data was used to assess differential methylation relating to the multifocal tumours, the epigenetic clock was used to assess the ‘timing’ of tumour development and metabolic traits predicted by DNA methylation were compared between samples.

    Article Snippet: Genome-wide DNA methylation microarray data was generated on the Illumina iScan System using the Illumina Infinium MethylationEPIC BeadChip according to the manufacturer’s protocol.

    Techniques: Genome Wide, DNA Methylation Assay, Methylation

    (A) Plot of age predictions for normal epithelia samples (green), primary tumours (purple) and metastatic tumours (blue). Chronological age is indicated with the orange points and patients are indicated in order of chronological age. (B) Boxplot of age acceleration difference for the skin and blood clock predictions. (C) Somatic mutation count from tumours correlates with DNA methylation age in the skin and blood clock (p=0.0007).

    Journal: bioRxiv

    Article Title: Epigenetic investigation of multifocal small intestinal neuroendocrine tumours reveals accelerated ageing of tumours and epigenetic alteration of metabolic genes

    doi: 10.1101/2024.12.02.626017

    Figure Lengend Snippet: (A) Plot of age predictions for normal epithelia samples (green), primary tumours (purple) and metastatic tumours (blue). Chronological age is indicated with the orange points and patients are indicated in order of chronological age. (B) Boxplot of age acceleration difference for the skin and blood clock predictions. (C) Somatic mutation count from tumours correlates with DNA methylation age in the skin and blood clock (p=0.0007).

    Article Snippet: Genome-wide DNA methylation microarray data was generated on the Illumina iScan System using the Illumina Infinium MethylationEPIC BeadChip according to the manufacturer’s protocol.

    Techniques: Mutagenesis, DNA Methylation Assay

    Sociodemographic and sexual characteristics of the incarcerated women in São Paulo, Brazil.

    Journal: Frontiers in Public Health

    Article Title: Prevalence of sexually transmitted infections and human papillomavirus in cervical samples from incarcerated women in São Paulo, Brazil: a retrospective single-center study

    doi: 10.3389/fpubh.2024.1353845

    Figure Lengend Snippet: Sociodemographic and sexual characteristics of the incarcerated women in São Paulo, Brazil.

    Article Snippet: The STI prevalence was determined using a similar DNA microarray system, EUROArray STI (EUROIMMUN, Germany).

    Techniques:

    Prevalence of STIs among the imprisoned women. The y-axis has been segmented in order to display all results. STIs for which all women were negative for have been excluded.

    Journal: Frontiers in Public Health

    Article Title: Prevalence of sexually transmitted infections and human papillomavirus in cervical samples from incarcerated women in São Paulo, Brazil: a retrospective single-center study

    doi: 10.3389/fpubh.2024.1353845

    Figure Lengend Snippet: Prevalence of STIs among the imprisoned women. The y-axis has been segmented in order to display all results. STIs for which all women were negative for have been excluded.

    Article Snippet: The STI prevalence was determined using a similar DNA microarray system, EUROArray STI (EUROIMMUN, Germany).

    Techniques:

    IHC staining of the NQO1 protein on tissue microarray of cervical lesions. (A) NQO1 protein was weakly positive in cervical SCC. (B) NQO1 protein showed diffuse and strong cytoplasmic-positive staining in cervical SCC. (C) NQO1 protein was negative in normal cervical epithelia. (D) NQO1 protein was positive in SCC.

    Journal: BMC Cancer

    Article Title: NQO1 overexpression is associated with poor prognosis in squamous cell carcinoma of the uterine cervix

    doi: 10.1186/1471-2407-14-414

    Figure Lengend Snippet: IHC staining of the NQO1 protein on tissue microarray of cervical lesions. (A) NQO1 protein was weakly positive in cervical SCC. (B) NQO1 protein showed diffuse and strong cytoplasmic-positive staining in cervical SCC. (C) NQO1 protein was negative in normal cervical epithelia. (D) NQO1 protein was positive in SCC.

    Article Snippet: DNA was extracted from paraffin-embedded cervical lesion specimens using the High Pure PCR Template Preparation kit (Cat.11796828001, Roche, Penzberg, Germany), and HPV detection and genotyping were performed using a PCR-based HPV-DNA microarray system (Biomedlab, Seoul, South Korea).

    Techniques: Immunohistochemistry, Microarray, Staining

    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Article Snippet: To meet the demand for the rapid and accurate detection of Mtb and drug resistance, CapitalBio developed a DNA microarray gene detection system.

    Techniques: Microarray, Control, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Article Snippet: To meet the demand for the rapid and accurate detection of Mtb and drug resistance, CapitalBio developed a DNA microarray gene detection system.

    Techniques: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Article Snippet: To meet the demand for the rapid and accurate detection of Mtb and drug resistance, CapitalBio developed a DNA microarray gene detection system.

    Techniques: Comparison, Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Article Snippet: To meet the demand for the rapid and accurate detection of Mtb and drug resistance, CapitalBio developed a DNA microarray gene detection system.

    Techniques: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Article Snippet: To meet the demand for the rapid and accurate detection of Mtb and drug resistance, CapitalBio developed a DNA microarray gene detection system.

    Techniques: Microarray, Mutagenesis