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dna microarray analysis procedures  (Thermo Fisher)


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    Thermo Fisher dna microarray analysis procedures
    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on <t>DNA</t> <t>microarray</t> data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.
    Dna Microarray Analysis Procedures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+method/Deoxyribonucleic+acid/pmc12621471-417-8-29
    Average 99 stars, based on 1 article reviews
    dna microarray analysis procedures - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer"

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102445

    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.
    Figure Legend Snippet: Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Techniques Used: Expressing, Microarray

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    other:

    Article Title: Cell-only bioprinting of articular cartilage progenitor cells within a physically constraining support bath to engineer structurally organized grafts
    Article Snippet: DNA content was quantified after digestion using Quant-iTTM PicoGreen ® dsDNA Reagent and Kit (Molecular Probes, Biosciences).

    Polymerase Chain Reaction:

    Article Title: Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh
    Article Snippet: .. 7 μl of each PCR product was separated on a 1.5 % ( w / v ) agarose gel stained with SYBR Safe DNA Gel Stain (Invitrogen, USA). .. A 100 bp DNA Ladder (Promega, USA) was used as a molecular marker, and the bands were visualized using the Gel Doc XR+ Imaging System (Bio-Rad, USA).

    Article Title: Identifying the role of NLRP3 inflammasome in stroke progression and outcome before recanalization
    Article Snippet: .. PCR was performed with equal amounts of cyclic deoxyribonucleic acid and water control in the StepOnePlusTM Real-Time PCR System (Applied Biosystems) using the TaqMan Universal 2X PCR Master Mix (Applied Biosystems). ..

    Agarose Gel Electrophoresis:

    Article Title: Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh
    Article Snippet: .. 7 μl of each PCR product was separated on a 1.5 % ( w / v ) agarose gel stained with SYBR Safe DNA Gel Stain (Invitrogen, USA). .. A 100 bp DNA Ladder (Promega, USA) was used as a molecular marker, and the bands were visualized using the Gel Doc XR+ Imaging System (Bio-Rad, USA).

    Staining:

    Article Title: Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh
    Article Snippet: .. 7 μl of each PCR product was separated on a 1.5 % ( w / v ) agarose gel stained with SYBR Safe DNA Gel Stain (Invitrogen, USA). .. A 100 bp DNA Ladder (Promega, USA) was used as a molecular marker, and the bands were visualized using the Gel Doc XR+ Imaging System (Bio-Rad, USA).

    Picogreen Assay:

    Article Title: Production and Purification of Mesenchymal Stem Cell-Derived Extracellular Vesicles Using Preferential Exclusion Chromatography.
    Article Snippet: .7 Total Protein and Double-Stranded eoxyribonucleic Acid (dsDNA) Assay he protein content in the process fractions was quantified sing the Bradford total protein assay, employing the Coomassie rotein assay kit (Thermo Scientific). .. For the determination f total deoxyribonucleic acid (DNA) content in process fracions, the Quant-iT PicoGreen dsDNA Assay (Invitrogen) was tilized. ..

    Control:

    Article Title: Identifying the role of NLRP3 inflammasome in stroke progression and outcome before recanalization
    Article Snippet: .. PCR was performed with equal amounts of cyclic deoxyribonucleic acid and water control in the StepOnePlusTM Real-Time PCR System (Applied Biosystems) using the TaqMan Universal 2X PCR Master Mix (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Identifying the role of NLRP3 inflammasome in stroke progression and outcome before recanalization
    Article Snippet: .. PCR was performed with equal amounts of cyclic deoxyribonucleic acid and water control in the StepOnePlusTM Real-Time PCR System (Applied Biosystems) using the TaqMan Universal 2X PCR Master Mix (Applied Biosystems). ..

    DNA Purification:

    Article Title: Epidemiological and antibiotic susceptibility pattern of bacterial paronychial infection.
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from the bacterial isolates using a DNA purification kit (Thermo Scientific, Catalog No. K0512, Waltham, MA, USA) and stored at -20°C for further use. ..



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    Image Search Results


    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Journal: Materials Today Bio

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer

    doi: 10.1016/j.mtbio.2025.102445

    Figure Lengend Snippet: Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Article Snippet: The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ].

    Techniques: Expressing, Microarray

    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Mutagenesis

    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Article Snippet: Nevertheless, the CapitalBio DNA microarray method is still a very suitable method for detecting the drug resistance of Mtb .

    Techniques: Microarray, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Article Snippet: Nevertheless, the CapitalBio DNA microarray method is still a very suitable method for detecting the drug resistance of Mtb .

    Techniques: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Article Snippet: Nevertheless, the CapitalBio DNA microarray method is still a very suitable method for detecting the drug resistance of Mtb .

    Techniques: Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Article Snippet: Nevertheless, the CapitalBio DNA microarray method is still a very suitable method for detecting the drug resistance of Mtb .

    Techniques: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Article Snippet: Nevertheless, the CapitalBio DNA microarray method is still a very suitable method for detecting the drug resistance of Mtb .

    Techniques: Microarray, Mutagenesis