disk diffusion method (Clinical and Laboratory Standards Institute)

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Clinical and Laboratory Standards Institute disk diffusion method
Disk Diffusion Method, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/disk+diffusion+methods/disk+diffusion+method/pm27657910-108-3-10
Average 90 stars, based on 1 article reviews

disk diffusion method - by Bioz Stars, 2026-09

90/100 stars

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Cell Culture:

Article Title: Study of lug Operon, SCC mec Elements, Antimicrobial Resistance, MGEs, and STs of Staphylococcus lugdunensis Clinical Isolates Through Whole-Genome Sequencing.
Article Snippet: All S. lugdunensis isolates were identified using a Bruker Biotyper (database 2.0) matrix-assisted laser desorption ionization-timeof-flight mass spectrometry system (Bruker Daltonics, Bremen, Germany), according to the manufacturer’s instructions, and S. lugdunensis isolates were stored in a tryptic soy broth with 20% glycerol at −80 ◦C until further experiments. .. All confirmed strains were cultured on Brain-Heart Infusion (BHI) agar at 37 ◦C for 18–24 h. The antibiotic susceptibility of the strains were assessed using the disk diffusion method according to the recommendations of the Clinical and Laboratory Standards Institute [32], covering a range of antibiotics including penicillin (P), cefoxitin (FOX), clindamycin (CC), erythromycin (E), trimethoprim–sulfamethoxazole (SXT), and teicoplanin (TEC). ..

Diffusion-based Assay:

Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3
Article Snippet: The E. coli ATCC 15597 strain was used for the isolation and propagation of bacteriophages. .. The antimicrobial susceptibility testing was performed using the disk diffusion method proposed by the Clinical and Laboratory Standards Institute (CLSI). ..

Article Title: Isolation and characterization of a novel Escherichia phage phiEco273 and its efficacy as an antibiofilm agent.
Article Snippet: In recent years, Escherichia coli is frequently associated with food borne infections causing colibacillosis in both food animals and humans.. Therefore, to secure a safe and healthy foods, there is a need of an alternative strategy.. Bacteriophages due to their ability serve as a promising strategy for combating drug-resistant bacteria.

Article Title: Comparative genomic and virulence analyses of a novel sequence type 420 Streptococcus equi subspecies zooepidemicus isolated from donkey
Article Snippet: .. The antimicrobial susceptibility assay was conducted using the disk diffusion method following the guidelines of the Clinical and Laboratory Standards Institute (CLSI, 2017) [ ]. ..

Article Title: Comparative genomic and virulence analyses of a novel sequence type 420 Streptococcus equi subspecies zooepidemicus isolated from donkey.
Article Snippet: .. The antimicrobial susceptibility assay was conducted using the disk diffusion method following the guidelines of the Clinical and Laboratory Standards Institute (CLSI, 2017) [49]. ..

Article Title: Multi-drug resistance and diversity of mobile genetic elements in Escherichia coli isolated from migratory bird in Poyang Lake.
Article Snippet: 17 With the spread of antibiotic resistance genes such as blaCTX-M-2, dfrA1 and 18 blaNDM-1, the problem of drug resistance in E. coli is becoming increasingly 19 serious.. This study aimed to identify integrons genes and MGEs in E. coli isolated 20 from migratory birds' feces at Poyang Lake, Jiangxi Province, focusing on their role 21 in antimicrobial resistance (AMR).. The 114 isolated E. coli strains were tested by 22 standard disk diffusion method and genetic testing method.

Article Title: First record of methicillin-resistant Staphylococcus aureus (MRSA) isolated from the Meia Ponte River and effluent in Brazil: an analysis of 1198 isolates
Article Snippet: .. Antibiotic susceptibility tests were performed using the disk diffusion method, as outlined in the standard protocol published by the Clinical and Laboratory Standards Institute [ ] and the methodology described by Tsai et al. [ ]. .. Predicted MRSA isolates were cultured in BHI broth at 37 °C for 24 h. After adjusting to the 0.5 McFarland scale, the diluted medium was spread on Baird Parker agar base using a sterile swab to facilitate the fixation of the antibiotic discs (Laborclin, Pinhais, PR, Brazil).

Drug Susceptibility Assay:

other:

Article Title: Direct economic costs related to antimicrobial resistance in bloodstream infections isolated from newborns in a perinatal hospital in Peru
Article Snippet: The antimicrobial susceptibility was evaluated by the disk-diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute guidelines.

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