Solvent:Article Title: Reimagining oil recovery: Sustainable downstream processing of oleaginous yeasts for food applications.
Article Snippet: .. 95* (Yellapu et al., 2016) Chemical Deep eutectic solvent (DES) – Choline chloride-based DES R. mucilaginosa CCT3892 Chloroform / Methanol (Bligh and Dyer) 16* (maximum reported value) (da Costa et al., 2020) Mechanical High pressure homogenization (HPH) Cell Disruption efficiency = 95%a, 74%b S. podzolica DSM27192a A. porosum DSM27194b Chloroform / Methanol (Bligh and Dyer) 43.4a 14.1b (Gorte, Hollenbach, et al., 2020) 29 Bead Milling (BM) Cell Disruption efficiency = 93%a, 53%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 32.3a 20b 13.2c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)c Sonication Cell Disruption efficiency = 27%a, 8%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 20.7a N.D.b 8.1c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)cPhysical Gas pressurization (Carbon dioxide with 99% cell death at 4000 kPa) R. glutinis ATCC 15125 Water / Chloroform / Methanol (1:1.1:1.1 vol.) .. 17.2 (85*) (Howlader et al., 2019) 30 Microwave Y. lipolytica IFP29 (ATCC 20460) Chloroform / Methanol (Bligh and Dyer) 7.1 (Meullemiestre et al., 2016) Biological Enzymes – Hydrolase enzyme system produced from Trichoderma reesei (a fungus) Cell Disruption efficiency = 85% (w/w) C. oleaginosus ATCC 20509 Centrifugation 90* (Masri et al., 2019) Pulsed electric field (PEF) Y. lipolytica JMY5578a S. podzolica DSM 27192b Hexanea Water / Ethanol / Hexane (1:18:7.3 vol.)b 29*a 95*b (Drévillon et al., 2019)a (Gorte, Nazarova, et al., 2020)bElectrical High voltage electric discharge (HVED) Y. lipolytica JMY5578 Hexane 32* (Drévillon et al., 2019) * extraction efficiency / wt% per total lipids N.D. non determined 33 scalability potential • Addition of water as pressurizing media might lead to complications in purification Ultrasound assisted hexane extraction 30 min, 2 5 W/cm2, 1 bar and 20 0C 16.6 Mano-sonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 20 0C 26.7 Thermo-sonication assisted hexane extraction 30 min, 25 W/cm2, 1 bar and 55 0C 21.3 Thermo-Manosonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 55 0C R. toruloid es CECT 1137 29.3 • Risk of degradation • Ultrasound assisted reactors are expensive – limiting scalability • Application of higher temperature did not have a significant impact (Meullemie stre et al., 2017) *Yield from high-speed homogenisation assisted hexane extraction
Homogenization:Article Title: Reimagining oil recovery: Sustainable downstream processing of oleaginous yeasts for food applications.
Article Snippet: .. 95* (Yellapu et al., 2016) Chemical Deep eutectic solvent (DES) – Choline chloride-based DES R. mucilaginosa CCT3892 Chloroform / Methanol (Bligh and Dyer) 16* (maximum reported value) (da Costa et al., 2020) Mechanical High pressure homogenization (HPH) Cell Disruption efficiency = 95%a, 74%b S. podzolica DSM27192a A. porosum DSM27194b Chloroform / Methanol (Bligh and Dyer) 43.4a 14.1b (Gorte, Hollenbach, et al., 2020) 29 Bead Milling (BM) Cell Disruption efficiency = 93%a, 53%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 32.3a 20b 13.2c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)c Sonication Cell Disruption efficiency = 27%a, 8%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 20.7a N.D.b 8.1c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)cPhysical Gas pressurization (Carbon dioxide with 99% cell death at 4000 kPa) R. glutinis ATCC 15125 Water / Chloroform / Methanol (1:1.1:1.1 vol.) .. 17.2 (85*) (Howlader et al., 2019) 30 Microwave Y. lipolytica IFP29 (ATCC 20460) Chloroform / Methanol (Bligh and Dyer) 7.1 (Meullemiestre et al., 2016) Biological Enzymes – Hydrolase enzyme system produced from Trichoderma reesei (a fungus) Cell Disruption efficiency = 85% (w/w) C. oleaginosus ATCC 20509 Centrifugation 90* (Masri et al., 2019) Pulsed electric field (PEF) Y. lipolytica JMY5578a S. podzolica DSM 27192b Hexanea Water / Ethanol / Hexane (1:18:7.3 vol.)b 29*a 95*b (Drévillon et al., 2019)a (Gorte, Nazarova, et al., 2020)bElectrical High voltage electric discharge (HVED) Y. lipolytica JMY5578 Hexane 32* (Drévillon et al., 2019) * extraction efficiency / wt% per total lipids N.D. non determined 33 scalability potential • Addition of water as pressurizing media might lead to complications in purification Ultrasound assisted hexane extraction 30 min, 2 5 W/cm2, 1 bar and 20 0C 16.6 Mano-sonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 20 0C 26.7 Thermo-sonication assisted hexane extraction 30 min, 25 W/cm2, 1 bar and 55 0C 21.3 Thermo-Manosonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 55 0C R. toruloid es CECT 1137 29.3 • Risk of degradation • Ultrasound assisted reactors are expensive – limiting scalability • Application of higher temperature did not have a significant impact (Meullemie stre et al., 2017) *Yield from high-speed homogenisation assisted hexane extraction
Disruption:Article Title: Reimagining oil recovery: Sustainable downstream processing of oleaginous yeasts for food applications.
Article Snippet: .. 95* (Yellapu et al., 2016) Chemical Deep eutectic solvent (DES) – Choline chloride-based DES R. mucilaginosa CCT3892 Chloroform / Methanol (Bligh and Dyer) 16* (maximum reported value) (da Costa et al., 2020) Mechanical High pressure homogenization (HPH) Cell Disruption efficiency = 95%a, 74%b S. podzolica DSM27192a A. porosum DSM27194b Chloroform / Methanol (Bligh and Dyer) 43.4a 14.1b (Gorte, Hollenbach, et al., 2020) 29 Bead Milling (BM) Cell Disruption efficiency = 93%a, 53%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 32.3a 20b 13.2c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)c Sonication Cell Disruption efficiency = 27%a, 8%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 20.7a N.D.b 8.1c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)cPhysical Gas pressurization (Carbon dioxide with 99% cell death at 4000 kPa) R. glutinis ATCC 15125 Water / Chloroform / Methanol (1:1.1:1.1 vol.) .. 17.2 (85*) (Howlader et al., 2019) 30 Microwave Y. lipolytica IFP29 (ATCC 20460) Chloroform / Methanol (Bligh and Dyer) 7.1 (Meullemiestre et al., 2016) Biological Enzymes – Hydrolase enzyme system produced from Trichoderma reesei (a fungus) Cell Disruption efficiency = 85% (w/w) C. oleaginosus ATCC 20509 Centrifugation 90* (Masri et al., 2019) Pulsed electric field (PEF) Y. lipolytica JMY5578a S. podzolica DSM 27192b Hexanea Water / Ethanol / Hexane (1:18:7.3 vol.)b 29*a 95*b (Drévillon et al., 2019)a (Gorte, Nazarova, et al., 2020)bElectrical High voltage electric discharge (HVED) Y. lipolytica JMY5578 Hexane 32* (Drévillon et al., 2019) * extraction efficiency / wt% per total lipids N.D. non determined 33 scalability potential • Addition of water as pressurizing media might lead to complications in purification Ultrasound assisted hexane extraction 30 min, 2 5 W/cm2, 1 bar and 20 0C 16.6 Mano-sonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 20 0C 26.7 Thermo-sonication assisted hexane extraction 30 min, 25 W/cm2, 1 bar and 55 0C 21.3 Thermo-Manosonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 55 0C R. toruloid es CECT 1137 29.3 • Risk of degradation • Ultrasound assisted reactors are expensive – limiting scalability • Application of higher temperature did not have a significant impact (Meullemie stre et al., 2017) *Yield from high-speed homogenisation assisted hexane extraction
Sonication:Article Title: Reimagining oil recovery: Sustainable downstream processing of oleaginous yeasts for food applications.
Article Snippet: .. 95* (Yellapu et al., 2016) Chemical Deep eutectic solvent (DES) – Choline chloride-based DES R. mucilaginosa CCT3892 Chloroform / Methanol (Bligh and Dyer) 16* (maximum reported value) (da Costa et al., 2020) Mechanical High pressure homogenization (HPH) Cell Disruption efficiency = 95%a, 74%b S. podzolica DSM27192a A. porosum DSM27194b Chloroform / Methanol (Bligh and Dyer) 43.4a 14.1b (Gorte, Hollenbach, et al., 2020) 29 Bead Milling (BM) Cell Disruption efficiency = 93%a, 53%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 32.3a 20b 13.2c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)c Sonication Cell Disruption efficiency = 27%a, 8%b S. podzolica DSM27192a A. porosum DSM27194b Y. lipolytica IFP29 (ATCC 20460)c Chloroform / Methanol (Bligh and Dyer) 20.7a N.D.b 8.1c (Gorte, Hollenbach, et al., 2020)a,b (Meullemiestre et al., 2016)cPhysical Gas pressurization (Carbon dioxide with 99% cell death at 4000 kPa) R. glutinis ATCC 15125 Water / Chloroform / Methanol (1:1.1:1.1 vol.) .. 17.2 (85*) (Howlader et al., 2019) 30 Microwave Y. lipolytica IFP29 (ATCC 20460) Chloroform / Methanol (Bligh and Dyer) 7.1 (Meullemiestre et al., 2016) Biological Enzymes – Hydrolase enzyme system produced from Trichoderma reesei (a fungus) Cell Disruption efficiency = 85% (w/w) C. oleaginosus ATCC 20509 Centrifugation 90* (Masri et al., 2019) Pulsed electric field (PEF) Y. lipolytica JMY5578a S. podzolica DSM 27192b Hexanea Water / Ethanol / Hexane (1:18:7.3 vol.)b 29*a 95*b (Drévillon et al., 2019)a (Gorte, Nazarova, et al., 2020)bElectrical High voltage electric discharge (HVED) Y. lipolytica JMY5578 Hexane 32* (Drévillon et al., 2019) * extraction efficiency / wt% per total lipids N.D. non determined 33 scalability potential • Addition of water as pressurizing media might lead to complications in purification Ultrasound assisted hexane extraction 30 min, 2 5 W/cm2, 1 bar and 20 0C 16.6 Mano-sonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 20 0C 26.7 Thermo-sonication assisted hexane extraction 30 min, 25 W/cm2, 1 bar and 55 0C 21.3 Thermo-Manosonication assisted hexane extraction 30 min, 25 W/cm2, 2 bar and 55 0C R. toruloid es CECT 1137 29.3 • Risk of degradation • Ultrasound assisted reactors are expensive – limiting scalability • Application of higher temperature did not have a significant impact (Meullemie stre et al., 2017) *Yield from high-speed homogenisation assisted hexane extraction
other:Article Title: Akebia saponin D attenuates ulcerative colitis via targeting EGFR and remodeling gut microbiota homeostasis.
Article Snippet: Background: Ulcerative colitis (UC), a refractory subtype of inflammatory bowel disease (IBD), is clinically characterized by chronic abdominal pain and bloody hematochezia.. Although therapeutic interventions have advanced significantly in recent years, existing treatment modalities remain limited.. Akebia saponin D (ASD), a bioactive triterpenoid saponin extracted from the traditional medicinal herb Dipsacus asper, has been demonstrated potent multimodal bioactivity.
Adhesive:Article Title: ADHESION, BIOFILM, AND INVASION: INVESTIGATING THE VIRULENCE MECHANISMS OF Prevotella spp.
Article Snippet: .. The adhesive capacity of other P. intermedia strains has already been documented by some authors: Grenier and colleagues (1996) [ ] described that the BMH strain was able to bind to type IV and type I collagen, and Marre (2020) [ ] reported that an ATCC strain of P. intermedia is capable of binding to type I laminin; however, the adhesion mechanisms of this species have not yet been elucidated. ..
Binding Assay:Article Title: ADHESION, BIOFILM, AND INVASION: INVESTIGATING THE VIRULENCE MECHANISMS OF Prevotella spp.
Article Snippet: .. The adhesive capacity of other P. intermedia strains has already been documented by some authors: Grenier and colleagues (1996) [ ] described that the BMH strain was able to bind to type IV and type I collagen, and Marre (2020) [ ] reported that an ATCC strain of P. intermedia is capable of binding to type I laminin; however, the adhesion mechanisms of this species have not yet been elucidated. ..
Extraction:Article Title: Photoprotective and cryoprotective effect of the carotenoid bacterioruberin on peripheral blood mononuclear cells.
Article Snippet: Additional reagents used in the study included: phosphate-buffered saline (PBS) (Hyclone, Logan, Utah, USA), dimethyl sulfoxide (DMSO) (Corning, New York, USA), acridine orange (AO) (SigmaAldrich, St. Louis, Missouri, USA), ethidium bromide (EtBr) (Sigma-Aldrich, St. Louis, Missouri, USA), propidium iodide (PI) (Thermo Fisher Scientific, Waltham, Massachusetts, USA), and Oxazole Yellow PRO-1 (YO-PRO-1) (Thermo Fisher Scientific, Waltham, Massachusetts, USA). .. Bacterioruberin-rich carotenoid extract (BRCE) extraction, purification, and purity determination was performed using procedures described previously (Montero-Lobato et al. 2018; Giani and Martínez-Espinosa 2020; Giani et al. 2021, 2022) from H. mediterranei strain R-4 (American Type Culture Collection (ATCC) 33500) (ATCC, Manassas, Virginia, USA), obtained from the salt flats of Santa Pola (Alicante, Spain). ..
Purification:Article Title: Photoprotective and cryoprotective effect of the carotenoid bacterioruberin on peripheral blood mononuclear cells.
Article Snippet: Additional reagents used in the study included: phosphate-buffered saline (PBS) (Hyclone, Logan, Utah, USA), dimethyl sulfoxide (DMSO) (Corning, New York, USA), acridine orange (AO) (SigmaAldrich, St. Louis, Missouri, USA), ethidium bromide (EtBr) (Sigma-Aldrich, St. Louis, Missouri, USA), propidium iodide (PI) (Thermo Fisher Scientific, Waltham, Massachusetts, USA), and Oxazole Yellow PRO-1 (YO-PRO-1) (Thermo Fisher Scientific, Waltham, Massachusetts, USA). .. Bacterioruberin-rich carotenoid extract (BRCE) extraction, purification, and purity determination was performed using procedures described previously (Montero-Lobato et al. 2018; Giani and Martínez-Espinosa 2020; Giani et al. 2021, 2022) from H. mediterranei strain R-4 (American Type Culture Collection (ATCC) 33500) (ATCC, Manassas, Virginia, USA), obtained from the salt flats of Santa Pola (Alicante, Spain). ..
Probiotics:
|
ATCC is a verified supplier 
ATCC manufactures this product 