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semi automated kirby bauer disc diffusion technique against ampicillin  (Bio-Rad)


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    Bio-Rad semi automated kirby bauer disc diffusion technique against ampicillin
    Semi Automated Kirby Bauer Disc Diffusion Technique Against Ampicillin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/disk+diffusion+method+against+reference+strains/Ampicillin/pm40724008-132-9-69
    Average 95 stars, based on 427 article reviews
    semi automated kirby bauer disc diffusion technique against ampicillin - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: The Urinary Tract commensal Peptoniphilus spp. Encodes a Novel 17β-Hydroxysteroid Dehydrogenase
    Article Snippet: .. Strep-tagged proteins (ampicillin resistant plasmid) were purified using Strep-Tactin resin (IBA), with clarified lysate applied to a gravity-flow column and washed with binding buffer until no protein was detected using the Bio-Rad Bradford dye reagent (Bio-Rad), followed by elution with buffer containing 2.5 mM D-desthiobiotin (IBA). .. His-tagged proteins (kanamycin resistant plasmid) were purified using Ni-NTA resin (Qiagen), washed with binding buffer containing 5 mM imidazole to remove nonspecific proteins, and eluted using high-imidazole elution buffer.

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture.
    Article Snippet: The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture
    Article Snippet: For this purpose, the cells were made competent, and the plasmid was introduced into the competent cells following the protocol detailed in the Gene Pulser X-cell System user manual electroporation system (Bio-Rad, USA). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL −1 ) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Purification:

    Article Title: The Urinary Tract commensal Peptoniphilus spp. Encodes a Novel 17β-Hydroxysteroid Dehydrogenase
    Article Snippet: .. Strep-tagged proteins (ampicillin resistant plasmid) were purified using Strep-Tactin resin (IBA), with clarified lysate applied to a gravity-flow column and washed with binding buffer until no protein was detected using the Bio-Rad Bradford dye reagent (Bio-Rad), followed by elution with buffer containing 2.5 mM D-desthiobiotin (IBA). .. His-tagged proteins (kanamycin resistant plasmid) were purified using Ni-NTA resin (Qiagen), washed with binding buffer containing 5 mM imidazole to remove nonspecific proteins, and eluted using high-imidazole elution buffer.

    Binding Assay:

    Article Title: The Urinary Tract commensal Peptoniphilus spp. Encodes a Novel 17β-Hydroxysteroid Dehydrogenase
    Article Snippet: .. Strep-tagged proteins (ampicillin resistant plasmid) were purified using Strep-Tactin resin (IBA), with clarified lysate applied to a gravity-flow column and washed with binding buffer until no protein was detected using the Bio-Rad Bradford dye reagent (Bio-Rad), followed by elution with buffer containing 2.5 mM D-desthiobiotin (IBA). .. His-tagged proteins (kanamycin resistant plasmid) were purified using Ni-NTA resin (Qiagen), washed with binding buffer containing 5 mM imidazole to remove nonspecific proteins, and eluted using high-imidazole elution buffer.

    other:



    Incubation:

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture.
    Article Snippet: The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture
    Article Snippet: For this purpose, the cells were made competent, and the plasmid was introduced into the competent cells following the protocol detailed in the Gene Pulser X-cell System user manual electroporation system (Bio-Rad, USA). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL −1 ) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Fluorescence:

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture.
    Article Snippet: The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture
    Article Snippet: For this purpose, the cells were made competent, and the plasmid was introduced into the competent cells following the protocol detailed in the Gene Pulser X-cell System user manual electroporation system (Bio-Rad, USA). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL −1 ) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Microscopy:

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture.
    Article Snippet: The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL−1) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Article Title: Phenotypic and safety exploration of indigenous bacterial strains for probiotic applications in aquaculture
    Article Snippet: For this purpose, the cells were made competent, and the plasmid was introduced into the competent cells following the protocol detailed in the Gene Pulser X-cell System user manual electroporation system (Bio-Rad, USA). .. The electrophoresed colonies were plated onto Luria Bertani agar (Merck KGaA, Darmstadt, Germany) supplemented with ampicillin (50 mcg.mL −1 ) and incubated at 30 °C for 18 h. The potential colonies were randomly picked and checked for plasmid insertion via visualization under a fluorescence microscope (ZOE fluorescent cell imager, Bio-Rad). ..

    Diffusion-based Assay:

    Article Title: Hypervirulent Klebsiella pneumoniae causing bloodstream infections in Hungary
    Article Snippet: .. The susceptibility of strains identified as hvKP to ampicillin, ampicillin-sulbactam, amoxicillin-clavulanate, piperacillin-tazobactam, cefuroxime, cefotaxime, ceftazidime, ceftriaxone, cefepime, ceftazidime-avibactam and ceftolozane-tazobactam, ertapenem, imipenem, meropenem, ciprofloxacin, levofloxacin, gentamicin, tobramycin, amikacin, trimethoprim-sulfamethoxazole, and cefiderocol was tested by Kirby–Bauer disk diffusion method on Mueller–Hinton agar (BioRad, Marnes-la-Coquette, France), according to the EUCAST guidelines ( ). .. The sequences of the complete genomes of strains identified as hvKP were determined by 150 bp paired-end sequencing on Illumina NovaSeq platform and long-read sequencing on Oxford Nanopore MinIon or on PacBio platforms.

    Selection:

    Article Title: A One Health Comparative Study of MDR Escherichia coli Isolated from Clinical Patients and Farm Animals in Satu Mare, Romania
    Article Snippet: Plates (50 μL antimicrobial + 50 μL inoculum per well) were sealed and incubated at 35 °C for 20 h. Minimum inhibitory concentrations (MICs) were read using a Biomic V3 analyzer (Giles Scientific Inc., San Diego, CA, USA) and interpreted using the same breakpoint standard(s) as above. .. All isolates (human and animal) were tested against: amikacin (AMK), ampicillin (AMP), cefotaxime (CTX), ceftazidime (CAZ), cefepime (FEP), ciprofloxacin (CIP), gentamicin (GEN), and sulfamethoxazole/trimethoprim (SXT) (Bio-Rad, Hercules, CA, USA), The selection of the antimicrobial substances was based on the Decision (EU) 2020/1729 of 17 November 2020, which specifies the antimicrobial classes to be included in national and EU-level AMR surveillance programs, ensuring comparability of data across countries and sectors Quality control included E. coli ATCC 25922. ..

    Control:

    Article Title: A One Health Comparative Study of MDR Escherichia coli Isolated from Clinical Patients and Farm Animals in Satu Mare, Romania
    Article Snippet: Plates (50 μL antimicrobial + 50 μL inoculum per well) were sealed and incubated at 35 °C for 20 h. Minimum inhibitory concentrations (MICs) were read using a Biomic V3 analyzer (Giles Scientific Inc., San Diego, CA, USA) and interpreted using the same breakpoint standard(s) as above. .. All isolates (human and animal) were tested against: amikacin (AMK), ampicillin (AMP), cefotaxime (CTX), ceftazidime (CAZ), cefepime (FEP), ciprofloxacin (CIP), gentamicin (GEN), and sulfamethoxazole/trimethoprim (SXT) (Bio-Rad, Hercules, CA, USA), The selection of the antimicrobial substances was based on the Decision (EU) 2020/1729 of 17 November 2020, which specifies the antimicrobial classes to be included in national and EU-level AMR surveillance programs, ensuring comparability of data across countries and sectors Quality control included E. coli ATCC 25922. ..



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