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dsp virus spin kit  (Qiagen)


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    Structured Review

    Qiagen dsp virus spin kit
    Data obtained from the chosen articles.
    Dsp Virus Spin Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/digital+spatial+profiling+spatial+transcriptomics+sequencing/QIAamp+DSP+Virus+Spin+Kit/pmc07886636-48-53-52
    Average 95 stars, based on 113 article reviews
    dsp virus spin kit - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Sampling methods and assays applied in SARS-CoV-2 exposure assessment"

    Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

    Journal: The Science of the Total Environment

    doi: 10.1016/j.scitotenv.2021.145903

    Data obtained from the chosen articles.
    Figure Legend Snippet: Data obtained from the chosen articles.

    Techniques Used: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

    Related Articles

    Virus:

    Article Title: Genomic profiling of AKT1 and NRAS mutations using NGS in Egyptian hepatocellular carcinoma
    Article Snippet: .. We next used the QIAamp ® DSP Virus Spin Kit, Version 1 (QIAGEN, Hilden, Germany) to extract cell-free DNA (cfDNA) from plasma according to the manufacturer’s instructions. .. This investigation was facilitated by the use of primers from the Ion AmpliSeq Cancer Hotspot Panel v2 (primer pool) (catalog number 4475346, Thermo Scientific, USA).

    Article Title: Association of Human Cytomegalovirus exposure with tuberculosis disease in South African adults with presumptive tuberculosis
    Article Snippet: .. HCMV DNAemia was determined by extracting viral DNA using QIAamp DSP Virus Spin kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Real-time qPCR was done using artus CMV RG PCR kit (Qiagen, Hilden, Germany) on the QuantStudio5 platform using the following parameters: hot start enzyme activation at 95°C for 10 minutes, denaturation at 95°C for 15 seconds, annealing at 65°C for 30 seconds, and extension at 72°C for 20 seconds.

    Article Title: A standardized protocol for the detection of arboviruses in different Aedes mosquito species in North Borneo Sabah, Malaysia
    Article Snippet: .. Reagents/tools , 1. Qiagen QIAamp DSP Virus Spin Kit 2. Smith Scientific Tissue Grinding Pestle 3. 70 % ethanol 4. Vortex mixer 5. Legend Micro 21 Centrifuge 6. BioRad CFX-96 Real-Time PCR. .. Experimental design , The experimental design investigated the potential vectors of arboviruses in forest areas, particularly for sylvatic transmission of dengue virus. Aedes mosquitoes were collected from rural areas and forests in North Borneo, Sabah, Malaysia. After collection, mosquitoes were identified and sorted into the species including Ae. aegypti, Ae. albopitcus, Ae. poecilus, Ae. butleri, Ae. niveus and Ae. vexans . The standard methods for the detection of arboviruses from mosquitoes usually involve the extraction of RNA from a pool of mosquitoes to obtain a sufficient RNA yield. This can be somewhat challenging if the samples do not reach the desired number, e.g. for relatively sparsely collected species such as Ae niveus . In addition, standard methods usually focus on the extraction of human blood or the primary dengue vector usually Ae. aegypti or albopictus , which may be different from extracting RNA from other Aedes mosquitoes. In addition, commercially available kits such as the QIAamp DSP Virus Spin Kit are primarily intended for use with liquid samples (e.g. serum and plasma) and do not include digestion steps required for solid tissues such as mosquito samples. To address these limitations, we have adapted and optimized the protocol to include the critical homogenization steps required for effective lysis of mosquito tissue. While these changes differ from the kit’s original protocol, they are fundamental to successful RNA extraction from insect vectors. These modifications help ensure the applicability of the protocol to different Aedes species. We implemented a single-tube workflow that simplified sample handling and reduced processing time and risk of contamination. The mosquito samples were processed individually or in fours, depending on the specific trap used to collect them with the corresponding GPS information recorded. We validated the extraction protocol using multiplex qPCR, which detects Dengue, Zika and Chikungunya viruses and compared the results to standard extraction methods. This detection method is highly sensitive and allows for the confirmation of viral RNA even in small or degraded samples. The combination of RNA extraction and multiplex qPCR enables faster and more accurate detection compared to conventional methods, further highlighting the utility of our protocol for arbovirus monitoring..

    Article Title: High hepatitis E virus prevalence in pig slurry samples from the north-western region of Germany
    Article Snippet: .. Viral RNA was extracted from a sample volume of 200 μL using the QiAmp MiniElute Virus Spin Kit (Qiagen, Hilden, Germany). .. HEV RNA copies were quantified by one-step quantitative real-time PCR (qRTPCR) (ViiATM 7 Real-Time PCR System Life Technologies, Carlsbad, CA) with HEV specific primers and probes (JVHEVF 5′GGTGGTTTCTGGGGTGAC; JVHEVR 5′AGGGGTTGGTTGGATGAA, TaqMan minor grove binding probe JVHEVPh 5′FAM-TGATTCTCAGCCCTTCGC-MGB).

    Article Title: A standardized protocol for the detection of arboviruses in different Aedes mosquito species in North Borneo Sabah, Malaysia
    Article Snippet: .. This protocol was adapted to the Qiagen QIAamp DSP Virus Spin Kit (Qiagen, Germany), which we used to further improve RNA recovery to ensure high sensitivity for virus detection even with a relatively small number of samples. ..

    Article Title: Genomic profiling of AKT1 and NRAS mutations using NGS in Egyptian hepatocellular carcinoma.
    Article Snippet: .. We next used the QIAamp® DSP Virus Spin Kit, Version 1 (QIAGEN, Hilden, Germany) to extract cell-free DNA (cfDNA) from plasma according to the manufacturer’s instructions. .. This investigation was facilitated by the use of primers from the Ion AmpliSeq Cancer Hotspot Panel v2 (primer pool) (catalog number 4475346, Thermo Scientific, USA).

    Article Title: High hepatitis E virus prevalence in pig slurry samples from the north-western region of Germany
    Article Snippet: The flow through was pipetted on top of an 8–40 % linear iodixanol/sucrose (OptiPrep, Progen Biotechnik, Heidelberg, Germany) gradient which was ultracentrifuged in a SW55 Ti rotor (Beckmann Coulter, Krefeld, Germany) at 43200 rpm and 4 ◦C for 18 h. 20 fractions á 250 μL were taken from bottom to top for each sample analyzed and the density was determined by refractometry. .. Viral RNA was purified from each fraction using the QiAmp MiniElute Virus Spin Kit (Qiagen) followed by qRT-PCR to quantify HEV RNA copy numbers as described above. ..

    Clinical Proteomics:

    Article Title: Genomic profiling of AKT1 and NRAS mutations using NGS in Egyptian hepatocellular carcinoma
    Article Snippet: .. We next used the QIAamp ® DSP Virus Spin Kit, Version 1 (QIAGEN, Hilden, Germany) to extract cell-free DNA (cfDNA) from plasma according to the manufacturer’s instructions. .. This investigation was facilitated by the use of primers from the Ion AmpliSeq Cancer Hotspot Panel v2 (primer pool) (catalog number 4475346, Thermo Scientific, USA).

    Article Title: Genomic profiling of AKT1 and NRAS mutations using NGS in Egyptian hepatocellular carcinoma.
    Article Snippet: .. We next used the QIAamp® DSP Virus Spin Kit, Version 1 (QIAGEN, Hilden, Germany) to extract cell-free DNA (cfDNA) from plasma according to the manufacturer’s instructions. .. This investigation was facilitated by the use of primers from the Ion AmpliSeq Cancer Hotspot Panel v2 (primer pool) (catalog number 4475346, Thermo Scientific, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: A standardized protocol for the detection of arboviruses in different Aedes mosquito species in North Borneo Sabah, Malaysia
    Article Snippet: .. Reagents/tools , 1. Qiagen QIAamp DSP Virus Spin Kit 2. Smith Scientific Tissue Grinding Pestle 3. 70 % ethanol 4. Vortex mixer 5. Legend Micro 21 Centrifuge 6. BioRad CFX-96 Real-Time PCR. .. Experimental design , The experimental design investigated the potential vectors of arboviruses in forest areas, particularly for sylvatic transmission of dengue virus. Aedes mosquitoes were collected from rural areas and forests in North Borneo, Sabah, Malaysia. After collection, mosquitoes were identified and sorted into the species including Ae. aegypti, Ae. albopitcus, Ae. poecilus, Ae. butleri, Ae. niveus and Ae. vexans . The standard methods for the detection of arboviruses from mosquitoes usually involve the extraction of RNA from a pool of mosquitoes to obtain a sufficient RNA yield. This can be somewhat challenging if the samples do not reach the desired number, e.g. for relatively sparsely collected species such as Ae niveus . In addition, standard methods usually focus on the extraction of human blood or the primary dengue vector usually Ae. aegypti or albopictus , which may be different from extracting RNA from other Aedes mosquitoes. In addition, commercially available kits such as the QIAamp DSP Virus Spin Kit are primarily intended for use with liquid samples (e.g. serum and plasma) and do not include digestion steps required for solid tissues such as mosquito samples. To address these limitations, we have adapted and optimized the protocol to include the critical homogenization steps required for effective lysis of mosquito tissue. While these changes differ from the kit’s original protocol, they are fundamental to successful RNA extraction from insect vectors. These modifications help ensure the applicability of the protocol to different Aedes species. We implemented a single-tube workflow that simplified sample handling and reduced processing time and risk of contamination. The mosquito samples were processed individually or in fours, depending on the specific trap used to collect them with the corresponding GPS information recorded. We validated the extraction protocol using multiplex qPCR, which detects Dengue, Zika and Chikungunya viruses and compared the results to standard extraction methods. This detection method is highly sensitive and allows for the confirmation of viral RNA even in small or degraded samples. The combination of RNA extraction and multiplex qPCR enables faster and more accurate detection compared to conventional methods, further highlighting the utility of our protocol for arbovirus monitoring..

    other:

    Article Title:
    Article Snippet: Comparison with predicate: Similarities and Differences Item Device Predicate FluChip-8G Influenza A+B Assay (K182513) CDC Human Influenza Virus Real-Time PCR Diagnostic Panel (K172091) Measurand Influenza RNA Same Viruses Detected Influenza A and B viruses Same Influenza A subtype Differentiation • Seasonal influenza A/H1N1pdm09 • Seasonal influenza A/H3N2 • “Non-seasonal” influenza A • Same • Same • Influenza A/H5 (Asian Lineage) Influenza B lineage Differentiation • Influenza B/Victoria • Influenza B/Yamagata Same Assay Type Qualitative Same CLIA Complexity High Same Sample Type Nasal swab and nasopharyngeal swab specimens Upper respiratory tract specimens, including nasal swabs and nasopharyngeal swabs; lower respiratory tract specimens Sample Preparation Method Nucleic Acid Extraction Same Detection Technology Fluorescence Same Technological Principles RT-PCR followed by endpoint detection by microarray via nucleic acid probes and fluorescence Real-time RT-PCR via nucleic acid probes and fluorescence Nucleic Acid Extraction QIAamp DSP Virus Spin Kit • QIAamp Viral RNA Mini Kit, Qiagen • QIAcube with QIAamp Viral RNA Mini Kit • MagNA Pure Compact—Total Nucleic Acid Kit, Roche • MagNA Pure Compact—RNA Isolation Kit, Roche • MagNA Pure LC—RNA Isolation Kit II, Roche • NucliSENS easyMAG, bioMerieux Controls • Internal Control in each specimen • External positive control processed with each batch • External negative control processed with each batch Same Amplification Reagents Quanta Biosciences qScript One-Step RTPCR Kit • Quanta Biosciences qScript One-Step qRT-PCR Kit, low ROX • Invitrogen SuperScript III Platinum One-Step Quantitative RT-PCR Kit Similarities and Differences Item Device Predicate FluChip-8G Influenza A+B Assay (K182513) CDC Human Influenza Virus Real-Time PCR Diagnostic Panel (K172091) Results Interpretation Automated test interpretation via neural network-based algorithm using fluorescence intensities Manual test interpretation via Ct value determined from fluorescence intensities Instrumentation FluChip-8G Imaging System Applied Biosystems 7500 Dx Real-Time PCR Instrument K. Standard/Guidance Document Referenced (if applicable): None L. Test Principle: The FluChip-8G Influenza A+B Assay (FC8G assay) includes a multiplex primer mix to amplify the hemagglutinin (HA), neuraminidase (NA), matrix (M), non-structural (NS), and nucleoprotein (NP) gene segments of all influenza A viruses, as well as the hemagglutinin (HA) and neuraminidase (NA) gene segments of all influenza B viruses, and a portion of the 18s gene present in eukaryotic cells as an endogenous internal control.

    Purification:

    Article Title: High hepatitis E virus prevalence in pig slurry samples from the north-western region of Germany
    Article Snippet: The flow through was pipetted on top of an 8–40 % linear iodixanol/sucrose (OptiPrep, Progen Biotechnik, Heidelberg, Germany) gradient which was ultracentrifuged in a SW55 Ti rotor (Beckmann Coulter, Krefeld, Germany) at 43200 rpm and 4 ◦C for 18 h. 20 fractions á 250 μL were taken from bottom to top for each sample analyzed and the density was determined by refractometry. .. Viral RNA was purified from each fraction using the QiAmp MiniElute Virus Spin Kit (Qiagen) followed by qRT-PCR to quantify HEV RNA copy numbers as described above. ..

    Quantitative RT-PCR:

    Article Title: High hepatitis E virus prevalence in pig slurry samples from the north-western region of Germany
    Article Snippet: The flow through was pipetted on top of an 8–40 % linear iodixanol/sucrose (OptiPrep, Progen Biotechnik, Heidelberg, Germany) gradient which was ultracentrifuged in a SW55 Ti rotor (Beckmann Coulter, Krefeld, Germany) at 43200 rpm and 4 ◦C for 18 h. 20 fractions á 250 μL were taken from bottom to top for each sample analyzed and the density was determined by refractometry. .. Viral RNA was purified from each fraction using the QiAmp MiniElute Virus Spin Kit (Qiagen) followed by qRT-PCR to quantify HEV RNA copy numbers as described above. ..



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