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diffusion pulse sequence bppled  (Bruker Corporation)

 
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    Structured Review

    Bruker Corporation diffusion pulse sequence bppled
    Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a <t>BPPLED</t> (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 <t>are</t> <t>diffusion</t> gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .
    Diffusion Pulse Sequence Bppled, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 97/100, based on 3085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diffusion+pulse+sequence+bppled/Diffusion/pmc12903051-68-3-2
    Average 97 stars, based on 3085 article reviews
    diffusion pulse sequence bppled - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Revealing Organophosphorus and Carbamate Interactions with Albumin Using 1 H NOE Pumping NMR Technique"

    Article Title: Revealing Organophosphorus and Carbamate Interactions with Albumin Using 1 H NOE Pumping NMR Technique

    Journal: Analytical Chemistry

    doi: 10.1021/acs.analchem.5c02132

    Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a BPPLED (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 are diffusion gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .
    Figure Legend Snippet: Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a BPPLED (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 are diffusion gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .

    Techniques Used: Sequencing, Blocking Assay, Activation Assay, Solvent, Diffusion-based Assay

    Related Articles

    Mass Spectrometry:

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: .. Gu (2024) [ ] , Skin swab , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test (Etest) , CLSI , PEN-susceptible. .. Lampejo (2024) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , Kirby Bauer disk diffusion test , EUCAST , CLI-, PEN-, VA-susceptible. MTZ-resistant.

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. Author Year Reference Type of Sample Method of Identification Method ofSusceptibility Testing Interpretation Criteria Antibiotics Resistance Phenotype (MIC mg/L) Chinello (2025) [28] Material drained from orbital cellulitis MALDI-TOF Mass Spectrometry NR NR AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016 Faiz (2025) [29] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test EUCAST 2023 breakpoint for Corynebacterium CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant Lovering (2025) [30] Blood culture MALDI-TOF MassSpectrometry NR NR CN, CRO, LZD, MEM, PEN, VA susceptible Saijo (2025) [31] Intraoperative sample from debridement MALDI-TOF Mass Spectrometry NR NR CRO 0.5, FEP 2, CTX 0.5, ERY < 0.12, PEN 0.25, RIF < 1, SXT < 0.5, VA 0.5 Bozso (2024) [32] Blood culture NR NR NR CLI ≤ 0.016, PEN ≤ 0.03 Chang (2024) [33] Blood culture, intraoperative samples from abscess NR NR NR NR Chin (2024) [34] Intraoperative sample from abscess NR NR NR NR Grusiecki (2024) [35] Purulent material from abscess NR NR NR PEN-susceptible CIP-, CLI-, MXF-resistant Gu (2024) [36] Skin swab MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test (Etest) CLSI PEN-susceptible https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Herai (2023) [ ] , Pleural fluid , MALDI-TOF Mass Spectrometry (Bruker) , NR , NR , NR. .. Sahhar (2023) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer diffusion test (Disks and Etest) , NR , CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible. .. Hicks (2022) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry , Kirby Bauer diffusion test (Etest) , NR , PEN 0.064.

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Chinello (2025) [ ] , Material drained from orbital cellulitis , MALDI-TOF Mass Spectrometry , NR , NR , AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016. .. Faiz (2025) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test , EUCAST 2023 breakpoint for Corynebacterium , CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant. .. Lovering (2025) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , NR , NR , CN, CRO, LZD, MEM, PEN, VA susceptible.

    Diffusion-based Assay:

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: .. Gu (2024) [ ] , Skin swab , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test (Etest) , CLSI , PEN-susceptible. .. Lampejo (2024) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , Kirby Bauer disk diffusion test , EUCAST , CLI-, PEN-, VA-susceptible. MTZ-resistant.

    Article Title: Potassium Hexafluoroacetylacetonate Complex with 18-Crown-6 Ether as a Volatile Precursor of Molecular and Inorganic Films: Thermal and Structural Insights.
    Article Snippet: The planar substrates of a cover glass for microscope slides (Minimed, Moscow, Russia, 0.15 × 18 × 18 mm3) and Si(100) (Telekom-STV JSC, Moscow, Russia, 0.7 × 17 × 17 mm3) were used for the molecular and inorganic film deposition, respectively. .. Diffusion coefficients were measured using the standard Bruker Ledbpgp2s sequence. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. Author Year Reference Type of Sample Method of Identification Method ofSusceptibility Testing Interpretation Criteria Antibiotics Resistance Phenotype (MIC mg/L) Chinello (2025) [28] Material drained from orbital cellulitis MALDI-TOF Mass Spectrometry NR NR AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016 Faiz (2025) [29] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test EUCAST 2023 breakpoint for Corynebacterium CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant Lovering (2025) [30] Blood culture MALDI-TOF MassSpectrometry NR NR CN, CRO, LZD, MEM, PEN, VA susceptible Saijo (2025) [31] Intraoperative sample from debridement MALDI-TOF Mass Spectrometry NR NR CRO 0.5, FEP 2, CTX 0.5, ERY < 0.12, PEN 0.25, RIF < 1, SXT < 0.5, VA 0.5 Bozso (2024) [32] Blood culture NR NR NR CLI ≤ 0.016, PEN ≤ 0.03 Chang (2024) [33] Blood culture, intraoperative samples from abscess NR NR NR NR Chin (2024) [34] Intraoperative sample from abscess NR NR NR NR Grusiecki (2024) [35] Purulent material from abscess NR NR NR PEN-susceptible CIP-, CLI-, MXF-resistant Gu (2024) [36] Skin swab MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test (Etest) CLSI PEN-susceptible https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Herai (2023) [ ] , Pleural fluid , MALDI-TOF Mass Spectrometry (Bruker) , NR , NR , NR. .. Sahhar (2023) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer diffusion test (Disks and Etest) , NR , CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible. .. Hicks (2022) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry , Kirby Bauer diffusion test (Etest) , NR , PEN 0.064.

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Chinello (2025) [ ] , Material drained from orbital cellulitis , MALDI-TOF Mass Spectrometry , NR , NR , AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016. .. Faiz (2025) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test , EUCAST 2023 breakpoint for Corynebacterium , CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant. .. Lovering (2025) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , NR , NR , CN, CRO, LZD, MEM, PEN, VA susceptible.

    Sequencing:

    Article Title: Potassium Hexafluoroacetylacetonate Complex with 18-Crown-6 Ether as a Volatile Precursor of Molecular and Inorganic Films: Thermal and Structural Insights.
    Article Snippet: The planar substrates of a cover glass for microscope slides (Minimed, Moscow, Russia, 0.15 × 18 × 18 mm3) and Si(100) (Telekom-STV JSC, Moscow, Russia, 0.7 × 17 × 17 mm3) were used for the molecular and inorganic film deposition, respectively. .. Diffusion coefficients were measured using the standard Bruker Ledbpgp2s sequence. ..

    Nuclear Magnetic Resonance:

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..

    Spectroscopy:

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..



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