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Triangle Biomedical diffquik stain kit
Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
Diffquik Stain Kit, supplied by Triangle Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diffquick+cell+staining+kit/rapi+diff+ii/pmc06826427-197-14-17
Average 90 stars, based on 1 article reviews
diffquik stain kit - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer"

Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer

Journal: Cancers

doi: 10.3390/cancers11101553

Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
Figure Legend Snippet: Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Techniques Used: Expressing, Invasion Assay, Transfection, Plasmid Preparation, Staining, Microscopy, Incubation, Fluorescence

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Article Title: Vaccine for prophylaxis or treatment of an allergen-driven airway pathology
Article Snippet: Total leukocytes and differential cell counts were performed as described, using Diff Quik/Rapi-Diff IITM (Triangle Biomedical Sciences, N.C.

Article Title: Vaccine for prophylaxis or treatment of an allergen-driven airway pathology
Article Snippet: Total leukocytes and differential cell counts were performed as described, using Diff Quik/Rapi-Diff IITM (Triangle Biomedical Sciences, NC, USA.).

Article Title: Vaccine for prophylaxis or treatment of an allergen-driven airway pathology
Article Snippet: Total leukocytes and differential cell counts were performed as described, using Diff Quik/Rapi-Diff IITM (Triangle Biomedical Sciences, NC., USA.).

Article Title: Vaccine for prophylaxis or treatment of an allergen-driven airway pathology
Article Snippet: Total leukocytes and differential cell counts were performed as described, using Diff Quik/Rapi-Diff IITM (Triangle Biomedical Sciences, NC, USA.).

Article Title: Corticosteroid insensitive alveolar macrophages from asthma patients; synergistic interaction with a p38 mitogen-activated protein kinase (MAPK) inhibitor
Article Snippet: Cytospins were prepared by cytocentrifugation at 7000 g , air-drying for 30 min, followed by methanol fixation and staining with RAPI-DIFF II (Triangle Biomedical Science, Durham, NC, USA).

Staining:

Article Title: The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway
Article Snippet: .. Differential Quik Stain Kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA) was used to stain the cells on the lower surface of the insert. ..

Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer
Article Snippet: .. After 24 h, the membranes were fixed with 100% methanol and stained with a DiffQuik stain kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA). .. The uninvaded cells were removed from the upper surface of the membrane, and the invaded cells were counted in six random high-power fields per filter using an Axio Imager M2 microscope (Carl Zeiss, Thornwood, NY, USA).

Article Title: Long Noncoding RNA ANRIL Promotes Cervical Cancer Development by Acting as a Sponge of miR-186
Article Snippet: .. The cells on the lower surface were stained with the Quik Stain Kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA). ..



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