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Microptic SL diff-quick staining
Diff Quick Staining, supplied by Microptic SL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick/diff+quick++staining/pmc07697408-77-19-21
Average 90 stars, based on 1 article reviews
diff-quick staining - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Diff-Quik:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Staining:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Concentration Assay:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Light Microscopy:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction