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Medion Diagnostics diff-quick staining
Diff Quick Staining, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick/diff+quick+staining/pmc03969542-96-1-3
Average 90 stars, based on 1 article reviews
diff-quick staining - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Isolation:

Article Title: Expansion microscopy of nuclear structure and dynamics in neutrophils
Article Snippet: Cells were counted and further diluted at the required concentration for the following procedures in RPMI 1640 containing 10mM HEPES (Roth) and 0.5% human serum albumin (Sigma Aldrich). .. Purity of the isolation was assessed by a cytospin assay (Cytospin 2 Zentrifuge, Shanson) and Diff Quick staining (Medion, Diagnostics). ..

Diff-Quik:

Article Title: Expansion microscopy of nuclear structure and dynamics in neutrophils
Article Snippet: Cells were counted and further diluted at the required concentration for the following procedures in RPMI 1640 containing 10mM HEPES (Roth) and 0.5% human serum albumin (Sigma Aldrich). .. Purity of the isolation was assessed by a cytospin assay (Cytospin 2 Zentrifuge, Shanson) and Diff Quick staining (Medion, Diagnostics). ..

Staining:

Article Title: Expansion microscopy of nuclear structure and dynamics in neutrophils
Article Snippet: Cells were counted and further diluted at the required concentration for the following procedures in RPMI 1640 containing 10mM HEPES (Roth) and 0.5% human serum albumin (Sigma Aldrich). .. Purity of the isolation was assessed by a cytospin assay (Cytospin 2 Zentrifuge, Shanson) and Diff Quick staining (Medion, Diagnostics). ..

Article Title: IL-6-elafin genetically modified macrophages as a lung immunotherapeutic strategy against Pseudomonas aeruginosa infections
Article Snippet: BALF supernatant was kept at 80 C until further use, e.g., for cytokine/chemokine measurement, with DuoSet enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems). .. Simultaneously, the BAL cell pellet was resuspended in 400 mL of PBS for cell type analysis using cytospin centrifugation and DiffQuik staining (Medion Diagnostics). .. In parallel, lungs were recovered in 1 mL of PBS and homogenized with a FastPrep-24 (MP Biomedicals) during two cycles (speed 6, 45 s).

other:

Article Title: Role of human group IIA secreted phospholipase A2 in malaria pathophysiology: Insights from a transgenic mouse model.
Article Snippet: Diff-Quick staining reagents were from Medion Diagnostics AG (Düdingen, Switzerland).

Article Title: Latent-transforming growth factor β-binding protein 1/Transforming growth factor β1 complex drives antitumoral effects upon ERK5 targeting in melanoma.
Article Snippet: After 24 hour-incubation cells that 185 had not migrated were removed with a cotton swab from the upper surface of filters and cells that had 186 migrated to the lower surface of the membrane were subjected to Diff-Quick staining (Medion 187 Jo urn al Pr e-p roo f 9 Diagnostics AG, Dudingen, Switzerland) and observed with a light microscope.

Article Title: Fibronectin induces macrophage migration through a SFK-FAK/CSF-1R pathway
Article Snippet: After incubation (1 or 2 hours for macrophage cell lines or primary macrophages, respectively) cells that had not migrated were removed with a cotton swab from the upper surface of filters, and cells that had migrated to the lower surface of the membrane were subjected to Diff-Quick staining (Medion Diagnostics AG, Dudingen, Switzerland) and observed with a light microscope.

Article Title: The RNA-binding protein KSRP reduces asthma-like characteristics in a murine model.
Article Snippet: Diff-Quick staining (Medion Grifols Diagnostics) was performed according to the manufacturers ́ protocol to differentiate the immune cell types.

Article Title: Safety and Effects of a Commercial Ozone Foam Preparation on Endometrial Environment and Fertility of Mares.
Article Snippet: Mares’ subfertility represents a complex diagnostic and therapeutic challenge and both clinical and subclinical endometritis are considered major causes of impaired fertility.. Thanks to its properties, ozone has a big potential as a treatment for equine endometritis.. Therefore, the aim of this study is to describe the safety and the effects on endometrium and reproductive parameters of mares of a commercial ozone foam preparation (Riger Spray®).

Article Title: Anti-Inflammatory Properties of Fructo-Oligosaccharides in a Calf Lung Infection Model and in Mannheimia haemolytica -Infected Airway Epithelial Cells
Article Snippet: After counting, 0.5 × 10 6 cells of BALF suspension were used to make cytospins and phenotyping was determined by Diff-Quick (Medion Diagnostics, Medion Diagnostics International Inc., Miami, FL, USA) staining on cytospin preparations and a minimum of 400 cells were counted.

Centrifugation:

Article Title: IL-6-elafin genetically modified macrophages as a lung immunotherapeutic strategy against Pseudomonas aeruginosa infections
Article Snippet: BALF supernatant was kept at 80 C until further use, e.g., for cytokine/chemokine measurement, with DuoSet enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems). .. Simultaneously, the BAL cell pellet was resuspended in 400 mL of PBS for cell type analysis using cytospin centrifugation and DiffQuik staining (Medion Diagnostics). .. In parallel, lungs were recovered in 1 mL of PBS and homogenized with a FastPrep-24 (MP Biomedicals) during two cycles (speed 6, 45 s).



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction